Atp6ap1 (BC048241) Mouse Tagged ORF Clone

CAT#: MG207392

  • TrueORF®

Atp6ap1 (tGFP-tagged) - Mouse ATPase, H+ transporting, lysosomal accessory protein 1 (cDNA clone MGC:54567 IMAGE:6493219)



Need custom modification / cloning service?
Get a free quote

Mouse Protein / Lysate Request

CNY 4,470.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms C7-1, 16A, CF2, ORF, AC45, XAP-3, VATPS1, mFLJ00383
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG207392 representing BC048241
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATGGCGGCAACAGTGGTATCTCGGATACGGACGGGGACAGGGCGGGCTCCAGTCATGTGGCTGTCTC
TGTCGTTGGTGGCAGTGGCAGCAGCAGTGGCCACGGAGCAGCAGGTGCCACTGGTACTGTGGTCGAGTGA
CCGGAATCTGTGGGCTCCTGTGGCCGACACACATGAGGGTCATATCACCAGCGATATGCAGCTTTCTACC
TACTTAGACCCTGCCCTGGAGCTGGGTCCCCGTAATGTACTGCTGTTCCTACAGGACAAGCTAAGCATTG
AGGATTTCACAGCATACGGTGGTGTGTTTGGAAATAAGCAGGACAGTGCCTTTTCTAACCTGGAGAATGC
CCTGGACTTGGCCCCCTCCTCACTGGTGCTTCCTGCTGTGGACTGGTATGCAATCAGCACTCTGACCACC
TACCTACAGGAGAAGCTTGGGGCTAGCCCCTTGCATGTGGATCTAGCTACCTTAAAGGAGCTGAAGCTCA
ATGCCAGCCTTCCTGCCCTGCTGCTCATCCGTCTGCCCTACACAGCCAGCTCCGGTCTGATGGCGCCCAG
GGAGGTCCTCACAGGTAACGATGAGGTCATCGGACAGGTACTGAGCACACTCAAGTCTGAAGATGTCCCT
TACACCGCAGCTCTTACTGCAGTCCGCCCTTCTAGAGTGGCCCGTGATATAACCATGGTGGCTGGGGGTC
TAGGTCGCCAGCTGCTTCAGACTCAGGTGGCATCTCCTGCGATCCATCCTCCTGTGAGTTACAATGACAC
TGCCCCAAGGATCCTGTTTTGGGCACAAAACTTCTCTGTGGCATACAAAGACGAGTGGAAAGACTTGACC
TCCCTCACCTTTGGTGTGGAGAATCTTAACTTGACCGGCTCCTTCTGGAATGACTCCTTTGCCATGCTTT
CACTGACCTATGAGCCACTCTTTGGTGCAACAGTGACATTCAAGTTCATTCTGGCTAGTCGCTTCTATCC
AGTGTCTGCCCGATATTGGTTTGCCATGGAACGACTTGAAATCCACAGCAATGGCTCTGTTGCCCATTTC
AATGTTTCCCAAGTCACAGGACCTAGCATCTATTCTTTCCACTGTGAGTACGTCAGCAGTGTGAGCAAGA
AAGGCAATCTCCTTGTGACCAACGTGCCCTCAGTCTGGCAGATGACTCTTCATAACTTCCAGATCCAGGC
CTTCAATGTGACTGGTGAACAGTTCTCCTATGCTAGCGACTGTGCTGGTTTCTTCTCTCCGGGCATCTGG
ATGGGTCTGCTCACCACCCTCTTCATGCTTTTCATATTCACCTATGGTCTGCACATGATACTCAGCCTAA
AGACCATGGATCGCTTTGATGACCACAAGGGCCCCACCATCACTTTGACCCAGATTGTG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG207392 representing BC048241
Red=Cloning site Green=Tags(s)

MMAATVVSRIRTGTGRAPVMWLSLSLVAVAAAVATEQQVPLVLWSSDRNLWAPVADTHEGHITSDMQLST
YLDPALELGPRNVLLFLQDKLSIEDFTAYGGVFGNKQDSAFSNLENALDLAPSSLVLPAVDWYAISTLTT
YLQEKLGASPLHVDLATLKELKLNASLPALLLIRLPYTASSGLMAPREVLTGNDEVIGQVLSTLKSEDVP
YTAALTAVRPSRVARDITMVAGGLGRQLLQTQVASPAIHPPVSYNDTAPRILFWAQNFSVAYKDEWKDLT
SLTFGVENLNLTGSFWNDSFAMLSLTYEPLFGATVTFKFILASRFYPVSARYWFAMERLEIHSNGSVAHF
NVSQVTGPSIYSFHCEYVSSVSKKGNLLVTNVPSVWQMTLHNFQIQAFNVTGEQFSYASDCAGFFSPGIW
MGLLTTLFMLFIFTYGLHMILSLKTMDRFDDHKGPTITLTQIV

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC048241
ORF Size 1389 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC048241, AAH48241
RefSeq Size 2126 bp
RefSeq ORF 1391 bp
Locus ID 54411
Gene Summary Accessory subunit of the proton-transporting vacuolar (V)-ATPase protein pump, which is required for luminal acidification of secretory vesicles. Guides the V-type ATPase into specialized subcellular compartments, such as neuroendocrine regulated secretory vesicles or the ruffled border of the osteoclast, thereby regulating its activity. Involved in membrane trafficking and Ca(2+)-dependent membrane fusion. May play a role in the assembly of the V-type ATPase complex. In aerobic conditions, involved in intracellular iron homeostasis, thus triggering the activity of Fe(2+) prolyl hydroxylase (PHD) enzymes, and leading to HIF1A hydroxylation and subsequent proteasomal degradation (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
Customer Reviews 
Loading...