Atp6ap1 (BC048241) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	C7-1, 16A, CF2, ORF, AC45, XAP-3, VATPS1, mFLJ00383
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG207392 representing BC048241 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGGCGGCAACAGTGGTATCTCGGATACGGACGGGGACAGGGCGGGCTCCAGTCATGTGGCTGTCTC TGTCGTTGGTGGCAGTGGCAGCAGCAGTGGCCACGGAGCAGCAGGTGCCACTGGTACTGTGGTCGAGTGA CCGGAATCTGTGGGCTCCTGTGGCCGACACACATGAGGGTCATATCACCAGCGATATGCAGCTTTCTACC TACTTAGACCCTGCCCTGGAGCTGGGTCCCCGTAATGTACTGCTGTTCCTACAGGACAAGCTAAGCATTG AGGATTTCACAGCATACGGTGGTGTGTTTGGAAATAAGCAGGACAGTGCCTTTTCTAACCTGGAGAATGC CCTGGACTTGGCCCCCTCCTCACTGGTGCTTCCTGCTGTGGACTGGTATGCAATCAGCACTCTGACCACC TACCTACAGGAGAAGCTTGGGGCTAGCCCCTTGCATGTGGATCTAGCTACCTTAAAGGAGCTGAAGCTCA ATGCCAGCCTTCCTGCCCTGCTGCTCATCCGTCTGCCCTACACAGCCAGCTCCGGTCTGATGGCGCCCAG GGAGGTCCTCACAGGTAACGATGAGGTCATCGGACAGGTACTGAGCACACTCAAGTCTGAAGATGTCCCT TACACCGCAGCTCTTACTGCAGTCCGCCCTTCTAGAGTGGCCCGTGATATAACCATGGTGGCTGGGGGTC TAGGTCGCCAGCTGCTTCAGACTCAGGTGGCATCTCCTGCGATCCATCCTCCTGTGAGTTACAATGACAC TGCCCCAAGGATCCTGTTTTGGGCACAAAACTTCTCTGTGGCATACAAAGACGAGTGGAAAGACTTGACC TCCCTCACCTTTGGTGTGGAGAATCTTAACTTGACCGGCTCCTTCTGGAATGACTCCTTTGCCATGCTTT CACTGACCTATGAGCCACTCTTTGGTGCAACAGTGACATTCAAGTTCATTCTGGCTAGTCGCTTCTATCC AGTGTCTGCCCGATATTGGTTTGCCATGGAACGACTTGAAATCCACAGCAATGGCTCTGTTGCCCATTTC AATGTTTCCCAAGTCACAGGACCTAGCATCTATTCTTTCCACTGTGAGTACGTCAGCAGTGTGAGCAAGA AAGGCAATCTCCTTGTGACCAACGTGCCCTCAGTCTGGCAGATGACTCTTCATAACTTCCAGATCCAGGC CTTCAATGTGACTGGTGAACAGTTCTCCTATGCTAGCGACTGTGCTGGTTTCTTCTCTCCGGGCATCTGG ATGGGTCTGCTCACCACCCTCTTCATGCTTTTCATATTCACCTATGGTCTGCACATGATACTCAGCCTAA AGACCATGGATCGCTTTGATGACCACAAGGGCCCCACCATCACTTTGACCCAGATTGTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG207392 representing BC048241 Red=Cloning site Green=Tags(s) MMAATVVSRIRTGTGRAPVMWLSLSLVAVAAAVATEQQVPLVLWSSDRNLWAPVADTHEGHITSDMQLST YLDPALELGPRNVLLFLQDKLSIEDFTAYGGVFGNKQDSAFSNLENALDLAPSSLVLPAVDWYAISTLTT YLQEKLGASPLHVDLATLKELKLNASLPALLLIRLPYTASSGLMAPREVLTGNDEVIGQVLSTLKSEDVP YTAALTAVRPSRVARDITMVAGGLGRQLLQTQVASPAIHPPVSYNDTAPRILFWAQNFSVAYKDEWKDLT SLTFGVENLNLTGSFWNDSFAMLSLTYEPLFGATVTFKFILASRFYPVSARYWFAMERLEIHSNGSVAHF NVSQVTGPSIYSFHCEYVSSVSKKGNLLVTNVPSVWQMTLHNFQIQAFNVTGEQFSYASDCAGFFSPGIW MGLLTTLFMLFIFTYGLHMILSLKTMDRFDDHKGPTITLTQIV TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC048241
ORF Size	1391 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC048241, AAH48241
RefSeq Size	2126 bp
RefSeq ORF	1391 bp
Locus ID	54411
Gene Summary	Accessory subunit of the proton-transporting vacuolar (V)-ATPase protein pump, which is required for luminal acidification of secretory vesicles. Guides the V-type ATPase into specialized subcellular compartments, such as neuroendocrine regulated secretory vesicles or the ruffled border of the osteoclast, thereby regulating its activity. Involved in membrane trafficking and Ca(2+)-dependent membrane fusion. May play a role in the assembly of the V-type ATPase complex. In aerobic conditions, involved in intracellular iron homeostasis, thus triggering the activity of Fe(2+) prolyl hydroxylase (PHD) enzymes, and leading to HIF1A hydroxylation and subsequent proteasomal degradation (By similarity).[UniProtKB/Swiss-Prot Function]

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