Chrnb3 (BC058193) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	5730417K16Rik; Acrb3
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG207420 representing BC058193 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACAGGCTTCCTACGGGTCTTCTTGGCTCTCAGTGCCACTCTCTCAGGTTCCTGGGTGACTCTCACAG CCACTGCGGGACTCAGCTCAGTGGCTGAACATGAAGACGCACTCCTCAGACATTTGTTCCAAGGTTATCA GAAATGTGTCCGCCCTGTGTTGAATTCCAGTGACATCATAAAAGTGTATTTTGGATTAAAGATATCTCAG CTTGTAGATGTGGATGAAAAGAATCAGCTGATGACAACAAACGTGTGGCTGAAGCAGGAATGGACAGACC AAAAATTACGATGGAATCCCGAAGACTATGGTGGAATTAATTCGATAAAGGTTCCATCGGAGTCGCTCTG GCTGCCTGACATAGTTCTCTTTGAAAATGCTGACGGACGTTTTGAGGGCTCTCTCATGACCAAGGCCATT GTGAAATCCAGCGGAACCGTCAGTTGGACTCCTCCCGCCAGCTACAAAAGTTCCTGTACCATGGATGTCA CATTTTTCCCGTTCGATAAGCAGAACTGCTCAATGAAGTTCGGATCCTGGACTTACGATGGTACCATGGT CGACCTCATTCTGATCAATGAAAACGTTGACCGGAAAGACTTTTTTGATAACGGAGAGTGGGAGATACTA AATGCAAAGGGGATGAAGGGCAACAGAAGAGAAGGCTTTTACTCCTATCCGTTTGTTACCTACTCTTTTG TTCTGAGACGCCTGCCCCTGTTTTACACCCTCTTTTTGATAATCCCCTGCCTGGGGCTGTCTTTTCTCAC GGTCCTGGTGTTCTACCTACCCTCCGACGAAGGGGAAAAACTCTCCTTATCCACCTCGGTTTTGGTTTCT TTGACAGTGTTTCTTTTAGTAATCGAAGAAATAATCCCGTCTTCTTCAAAAGTCATCCCTCTCATTGGGG AGTACCTCCTCTTCATTATGATATTTGTCACGCTGTCTATTATTGTCACGGTTTTTGTAATTAATGTCCA CCACAGATCTTCCTCAACGTACCATCCCATGGCCCCCTGGGTGAAGAGGCTGTTTCTGGAAAAACTCCCG AGATGGCTTTGCATGAAGGACCCCAGGGACCGCTTCTCTTTCCCCGATGGAACGGAGAGTAAGGGAACCG TGAGGGGGAAATTTCCAGGGAAAAAGAAGCAGACTCCTACCAGTGATGGAGAAAGAGTTCTGGTCGCTTT CCTGGAGAAGGCCTCTGAGTCCATCAGATACATCTCGAGGCATGTGAAGAAGGAACACTTCATCAGTCAG GTTGTTCAAGACTGGAAATTCGTGGCTCAGGTTCTTGACCGCATCTTCCTCTGGCTCTTTCTGACAGCTT CGGTTTTGGGCTCTGTCCTGATTTTTATTCCAGCCTTGAAGATGTGGATACATCGTTTCCAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG207420 representing BC058193 Red=Cloning site Green=Tags(s) MTGFLRVFLALSATLSGSWVTLTATAGLSSVAEHEDALLRHLFQGYQKCVRPVLNSSDIIKVYFGLKISQ LVDVDEKNQLMTTNVWLKQEWTDQKLRWNPEDYGGINSIKVPSESLWLPDIVLFENADGRFEGSLMTKAI VKSSGTVSWTPPASYKSSCTMDVTFFPFDKQNCSMKFGSWTYDGTMVDLILINENVDRKDFFDNGEWEIL NAKGMKGNRREGFYSYPFVTYSFVLRRLPLFYTLFLIIPCLGLSFLTVLVFYLPSDEGEKLSLSTSVLVS LTVFLLVIEEIIPSSSKVIPLIGEYLLFIMIFVTLSIIVTVFVINVHHRSSSTYHPMAPWVKRLFLEKLP RWLCMKDPRDRFSFPDGTESKGTVRGKFPGKKKQTPTSDGERVLVAFLEKASESIRYISRHVKKEHFISQ VVQDWKFVAQVLDRIFLWLFLTASVLGSVLIFIPALKMWIHRFH TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC058193
ORF Size	1394 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC058193, AAH58193
RefSeq Size	4454 bp
RefSeq ORF	1394 bp
Locus ID	108043
Gene Summary	After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.[UniProtKB/Swiss-Prot Function]

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