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Adhfe1 (NM_175236) Mouse Tagged ORF Clone
CAT＃: MG207433
- TrueORF®
Adhfe1 (tGFP-tagged) - Mouse alcohol dehydrogenase, iron containing, 1 (Adhfe1)
ORF Plasmid: DDK
"NM_175236" in other vectors (4)
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CNY 3,710.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 6330565B14Rik; Adh8; AI043035; HOT |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG207433 representing NM_175236
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCAGCTGCACGCGCTCGAGTCACACACTTGCTGAGGCACCTACAAAGCACAGCATGCCAGTGCC CAACTCATTCTCATACTTACTCCCAAGCCCCTGGACCTTCGGGAAAAACAGCAGATTACGCCTTTGAGAT GGCTGTCTCAAATATTAGATATGGAGCAGGGGTTACGAAGGAAGTAGGCATGGATCTACAGAATATGGGA GCTAAGAATGTTTGCTTGATGACAGACAAGAACCTCTCCCAGCTCCCTCCTGTACAAATAGTTATGGATT CCCTATCGAAGAATGGCATCAGTTTTCAGGTTTATGATGATGTAAGGGTGGAACCTACAGATGGAAGCTT CATGGATGCAATTGAATTTGCCAAAAAGGGAGCGTTTGATGCCTATGTTGCTGTGGGTGGGGGCTCCACC ATGGACACCTGTAAAGCCGCCAATCTGTATGCATCCAGCCCTCACTCTGAGTTCCTTGATTATGTCAATG CCCCCATTGGGAAGGGGAAGCCAGTAACTGTGCCTCTTAAACCTCTGATTGCAGTTCCAACTACCTCAGG GACTGGCAGTGAGACTACCGGGGTTGCCATTTTTGACTATGAGCATTTGAAAGTAAAAACTGGCATTGCT TCACGAGCCATCAAACCCACCCTGGGACTGGTTGATCCCCTGCACACTCTCCACATGCCTTGCCAGGTGG TTGCCAACAGTGGCTTCGATGTCCTTTGCCATGCTCTGGAGTCATACACCGCCATTCCCTACAGCATGCG GAGCCCCTGCCCTTCCAACCCCATCCAACGACCAGCATACCAGGGTAGCAACCCAATCAGCGACATCTGG GCAGTCCATGCACTGCAGATTGTTGCCAAATATCTGAAGAGGGCTGTCAGAAACCCAGATGATCTGGAAG CAAGGTCTAAAATGCACTTGGCAAGTGCTTTTGCTGGCATTGGCTTCGGAAATGCTGGTGTTCATCTGTG CCATGGCATGTCTTACCCAATTTCAGGTTTAGTGAAGACATATAAAGCCAAGGAATATAATGTGGATCAC CCTCTTGTGCCCCATGGCCTTTCTGTGGTGCTCACCTCTCCTGCAGTGTTCACCTTCACAGCCCAGATGT TTCCAGAGCGGCACCTGGAGACGGCAGGAATATTAGGAGCCAACATCCGCACTGCCAGGATCCAAGATGC TGGGCTTGTGTTGGCAGATGCCCTCCGAAAATTCCTATTTGACCTAAATGTTGATGACGGTCTGGCTGCT CTTGGTTATTCCAAGGATGACATTCCTTCATTGGTGAAAGGAACACTGCCCCAGGAAAGGGTCACGAAGC TCGCACCTCGTGCCCAGTCAGAAGAGGATTTGTCTGCTTTGTTTGAAGCATCCATGAAACTGTAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG207433 representing NM_175236
Red=Cloning site Green=Tags(s) MAAAARARVTHLLRHLQSTACQCPTHSHTYSQAPGPSGKTADYAFEMAVSNIRYGAGVTKEVGMDLQNMG AKNVCLMTDKNLSQLPPVQIVMDSLSKNGISFQVYDDVRVEPTDGSFMDAIEFAKKGAFDAYVAVGGGST MDTCKAANLYASSPHSEFLDYVNAPIGKGKPVTVPLKPLIAVPTTSGTGSETTGVAIFDYEHLKVKTGIA SRAIKPTLGLVDPLHTLHMPCQVVANSGFDVLCHALESYTAIPYSMRSPCPSNPIQRPAYQGSNPISDIW AVHALQIVAKYLKRAVRNPDDLEARSKMHLASAFAGIGFGNAGVHLCHGMSYPISGLVKTYKAKEYNVDH PLVPHGLSVVLTSPAVFTFTAQMFPERHLETAGILGANIRTARIQDAGLVLADALRKFLFDLNVDDGLAA LGYSKDDIPSLVKGTLPQERVTKLAPRAQSEEDLSALFEASMKLY TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_175236 |
| ORF Size | 1395 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_175236.5 |
| RefSeq Size | 3108 bp |
| RefSeq ORF | 1398 bp |
| Locus ID | 76187 |
| UniProt ID | Q8R0N6 |
| Gene Summary | Catalyzes the cofactor-independent reversible oxidation of gamma-hydroxybutyrate (GHB) to succinic semialdehyde (SSA) coupled to reduction of 2-ketoglutarate (2-KG) to D-2-hydroxyglutarate (D-2-HG). L-3-hydroxybutyrate (L-3-OHB) is also a substrate for HOT when using 2-KG as hydrogen acceptor, resulting in the formation of D-2-HG (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC202122 | Adhfe1 (untagged) - Mouse alcohol dehydrogenase, iron containing, 1 (Adhfe1), nuclear gene encoding mitochondrial protein, transcript variant 1, (10ug) |
CNY 3,656.00 |
|
| MR207433 | Adhfe1 (Myc-DDK-tagged) - Mouse alcohol dehydrogenase, iron containing, 1 (Adhfe1), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 3,656.00 |
|
| MR207433L3 | Lenti ORF clone of Adhfe1 (Myc-DDK-tagged) - Mouse alcohol dehydrogenase, iron containing, 1 (Adhfe1), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 6,056.00 |
|
| MR207433L4 | Lenti ORF clone of Adhfe1 (mGFP-tagged) - Mouse alcohol dehydrogenase, iron containing, 1 (Adhfe1), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 5,230.00 |
