Lmbrd1 (BC025579) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	0910001K20Rik; AV347960
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG207473 representing BC025579 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAAAATCAAAATGGCACATTCAAGGACTGGGCTGACGCCAATGTCACCGTACAGATTGAGAATACCG TTCTGTATGGCTACTATACTCTGTATTCTGTCATTCTCTTCTGTGTGTTCTTCTGGATCCCGTTTGTGTA CTTCTACTACGAAGAGAAGGATGAGGACGACGCCAGCAAATGCACCCAAATTAAAACGGCGCTCAAGTAC ACTTTGGGATTTGTTGTGATCTGCGCTCTGCTGCTTCTCGTTGGTGCCTTTGTGCCTCTGCATCTGCCCA ACAACAACAACTCAACAGAGTGGGAAAAGGTGAAGCTCCTGTTTGAAGACCTTGGAACTGGTCAAGGCCT GGCAGCTCTGTCCTTTTCCATCAGCTCTCTGACCTTGATTGGGATGCTGGCGGCCATAACCTACACAGCG TACGGCATGTCGGCATTACCTTTAAACCTAATCAAAGGCACTAGAAGCACTGCCTACGAGCGCCTGGAGA ACACGGAGGACATCGAGGAAGTGGAGCAGCACATTCAGACGATTCGATCCAAGAGCAAAGATGGTCGACC TTTGCCAGCGAGGGATAGACGTGCCTTAAAGCAATGTGAAGAAAGACTAAGAACGCTTAGGAAAAGAGAG CGGCACTTGGAATTCATTGAAAACAGCTGGTGGACGAAATTTTGTGGTGCCCTGCGCCCCCTGAAGATCA TTTGGGGAATATTTTTCATCCTCGTTGCGTTGCTGTTTGTAATTTCTCTCTTCCTGTCAAATTTGGATAA AGCTCTTCATTCAGCAGGAATAGATTCGGGTTTCATCATCTTCGGAACTAACCTGAGCAATCCACTGAAC ATGCTTTTGCCGTTGTTACAAACAGTGTTCCCTCTTGATTACATTCTTATAACAATTATTATTATGTACT TTATTTTTACTTCGATGGCAGGAATTCGAAACATTGGCATTTGGTTCTTTTGGATTAGGTTATATAAAAT CAGAAGAGGGCGAACCAGGCCTCAGGCACTCTTATTTCTCTGCATGATCCTCCTGCTCATTGTCCTTCAT ACCAGCTACATGATCTACAGTCTCGCGCCTCAGTACGTGATGTACGGAAGCCAGAATTACTTAATAGAGA GCAATATAACTTCTGATGCCCATAAAGGCAATTCAACCCTCGCTGTGCCAAAAAGATGTGACGCAGATGC CCCTAAAGATCAGTGTACTGTTACCCGAACATACATTTTCCTCCACAAGTTCTGGTTCTTCAGTGCTGCA TACTATTTTGGTAACTGGGCCTTTCTCGTGGTGTTCTTGATCGGATTAATTGTATCCTGCTGTAAAGGGA AGAAGTCAGTCATAGAAGGAGTGGATGAAGATTCGGACTTAAGTGATGATGAGCCGTCTGCCTACTCTGC G ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG207473 representing BC025579 Red=Cloning site Green=Tags(s) MKNQNGTFKDWADANVTVQIENTVLYGYYTLYSVILFCVFFWIPFVYFYYEEKDEDDASKCTQIKTALKY TLGFVVICALLLLVGAFVPLHLPNNNNSTEWEKVKLLFEDLGTGQGLAALSFSISSLTLIGMLAAITYTA YGMSALPLNLIKGTRSTAYERLENTEDIEEVEQHIQTIRSKSKDGRPLPARDRRALKQCEERLRTLRKRE RHLEFIENSWWTKFCGALRPLKIIWGIFFILVALLFVISLFLSNLDKALHSAGIDSGFIIFGTNLSNPLN MLLPLLQTVFPLDYILITIIIMYFIFTSMAGIRNIGIWFFWIRLYKIRRGRTRPQALLFLCMILLLIVLH TSYMIYSLAPQYVMYGSQNYLIESNITSDAHKGNSTLAVPKRCDADAPKDQCTVTRTYIFLHKFWFFSAA YYFGNWAFLVVFLIGLIVSCCKGKKSVIEGVDEDSDLSDDEPSAYSA TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC025579
ORF Size	1403 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC025579, AAH25579
RefSeq Size	4386 bp
RefSeq ORF	1403 bp
Locus ID	68421
Gene Summary	Probable lysosomal cobalamin transporter. Required to export cobalamin from lysosomes allowing its conversion to cofactors (By similarity).[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.