Ppara (BC016892) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	Nr1c1, PPAR-alpha, PPARalpha
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG207486 representing BC016892 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGGACACAGAGAGCCCCATCTGTCCTCTCTCCCCACTGGAGGCAGATGACCTGGAAAGTCCCTTAT CTGAAGAATTCTTACAAGAAATGGGAAACATTCAAGAGATTTCTCAGTCCATCGGTGAGGAGAGCTCTGG AAGCTTTGGTTTTGCAGACTACCAGTACTTAGGAAGCTGTCCGGGCTCCGAGGGCTCTGTCATCACAGAC ACCCTCTCTCCAGCTTCCAGCCCTTCCTCAGTCAGCTGCCCCGTGATCCCCGCCAGCACGGACGAGTCCC CCGGCAGTGCCCTGAACATCGAGTGTCGAATATGTGGGGACAAGGCCTCAGGGTACCACTACGGAGTTCA CGCATGTGAAGGCTGTAAGGGCTTCTTTCGGCGAACTATTCGGCTGAAGCTGGTGTACGACAAGTGTGAT CGGAGCTGCAAGATTCAGAAGAAGAACCGGAACAAATGCCAGTACTGCCGTTTTCACAAGTGCCTGTCTG TCGGGATGTCACACAATGCAATTCGCTTTGGAAGAATGCCAAGATCTGAAAAAGCAAAACTGAAAGCAGA AATTCTTACCTGTGAACACGACCTGAAAGATTCGGAAACTGCAGACCTCAAATCTCTGGGCAAGAGAATC CACGAAGCCTACCTGAAGAACTTCAACATGAACAAGGTCAAGGCCCGGGTCATACTCGCGGGAAAGACCA GCAACAACCCGCCTTTTGTCATACATGACATGGAGACCTTGTGTATGGCCGAGAAGACGCTTGTGGCCAA GATGGTGGCCAACGGCGTCGAAGACAAAGAGGCAGAGGTCCGATTCTTCCACTGCTGCCAGTGCATGTCC GTGGAGACCGTCACGGAGCTCACAGAATTTGCCAAGGCTATCCCAGGCTTTGCAAACTTGGACTTGAACG ACCAAGTCACCTTGCTAAAGTACGGTGTGTATGAAGCCATCTTCACGATGCTGTCCTCCTTGATGAACAA AGACGGGATGCTGATCGCGTACGGCAATGGCTTTATCACACGCGAGTTCCTTAAGAACCTGAGGAAGCCG TTCTGTGACATCATGGAACCCAAGTTTGACTTCGCTATGAAGTTCAATGCCTTAGAACTGGATGACAGTG ACATTTCCCTGTTTGTGGCTGCTATAATTTGCTGTGGAGATCGGCCTGGCCTTCTAAACATAGGCTACAT TGAGAAGTTGCAGGAGGGGATTGTGCACGTGCTTAAGCTCCACCTGCAGAGCAACCATCCAGATGACACC TTCCTCTTCCCAAAGCTCCTTCAAAAAATGGTGGACCTTCGGCAGCTGGTCACGGAGCATGCGCAGCTCG TACAGGTCATCAAGAAGACCGAGTCCGACGCAGCGCTGCACCCACTGTTGCAAGAGATCTACAGAGACAT GTAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC016892
ORF Size	1404 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC016892, AAH16892
RefSeq Size	2336 bp
RefSeq ORF	1406 bp
Locus ID	19013
Gene Summary	Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety. Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2. May be required for the propagation of clock information to metabolic pathways regulated by PER2.[UniProtKB/Swiss-Prot Function]

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