Terf2 (BC046284) Mouse Tagged ORF Clone

CAT#: MG207518

  • TrueORF®

Terf2 (tGFP-tagged) - Mouse telomeric repeat binding factor 2 (cDNA clone MGC:54756 IMAGE:6439629)



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Mouse Protein / Lysate Request

CNY 3,710.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms TRF2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG207518 representing BC046284
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGCGGGAGCCGGGACAGCGGGCCCTGCTTCCGGCCCGGGCGTTGTGCGTGACCCGATGGCGTCAC
AGCCAAGGAAACGGCCCAGTCGGGAGGGCGGGGAGGGCGGGGAGGGCGAGCGGCGGTCGAACACGATGGC
GGGAGGAGGCGGGAGCAGCGATAGCAGCGGGCGGGCGGCGAGCCGACGGGCATCGCGCAGCGGCGGGCGG
GCTCGACGGGGGCGACACGAGCCAGGGTTGGGAGGCGCGGCCGAGCGGGGCGCGGGGGAAGCTCGCCTGG
AGGAGGCGGTCAACCGCTGGGTGCTCAAGTTCTATTTCCACGAGGCGCTGCGGGCCTTTCGGAGTAGCCG
GTACCGGGACTTCAGGCAGATCCGGGACATCATGCAGGCGTTGCTTGTCAGGCCCTTGGGGAAGGAGCAT
ACGGTGTCCCGGTTGCTGCGGGTTATGCAGTGTCTGTCGCGCATTGAAGAAGGAGAAAATTTAGACTGTT
CCTTTGATATGGAGGCTGAGCTCACACCCTTGGAATCAGCTATCAATGTGCTGGAGATGATTAAAACAGA
GTTCACACTGACAGACTCTATGGTTGAATCCAGCAGAAAACTGGTCAAGGAGGCTGCTGTCATTATTTGT
ATCAAAAACAAAGAATTTGAAAAGGCTTCAAAGATTTTGAAAAAATACATGTCTAAGGACCCCACAACTC
AGAAGCTGAGAACTGATCTCCTGAACATTATCCGGGAAAAGAACTTGGCCCACCCTGTTATCCAGAACTT
TTCCTATGAGGTCTTCCAGCAGAAGATGCTGCGTTTCCTAGAGAGCCACCTGGATGACACGGAGCCCTAC
CTCCTCACGATGGCTAAAAAAGCTTTGAAATCTGAATCAGCTGCTTCAAGTACAATGAGGGAAGAAAAGC
ACCCAGAGCCAGTGGAAAAACCACTTAGAGAGCCTCCAAGCAGACAGCCTCAGAACCCTCCAGCCACCAT
CGGGATCAGGACTCTGAAGGCAGCTTTCAAAGCTCTGTCTACTGCACAAGACTCAGAGGCCGCTTTTGCA
AAACTGGACCAGAAAGATCTGGTACTTGCTAATCTGGCATCCCCATCATCACCAGCCCACAAACACAAGA
GACCCAGGAAAGATGAACATGAAAGCGCAGCTCCTGCTGAGGGTGAGGGAGGCTCAGACCGGCAGCCCAG
GAACAGTCCCATGACAATAAGCAGATTGCTGTTGGAGGAGGACAGCCAGAGTACTGAGCCCAGCCCAGGC
CTCAACTCCTCCCACGAGGCCATGTCAGCATCCAAGCCCAGAGCTCTCAACCAACCCCACCCGGGGGAGA
AGAAGCCCAAGTATGAAGACCTTCTTTGTAGGAGTTTGGGGGCTGGTTGGCGGGCCTGGTTGGGCCTGGT
TTTACTTCCA


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG207518 representing BC046284
Red=Cloning site Green=Tags(s)

MAAGAGTAGPASGPGVVRDPMASQPRKRPSREGGEGGEGERRSNTMAGGGGSSDSSGRAASRRASRSGGR
ARRGRHEPGLGGAAERGAGEARLEEAVNRWVLKFYFHEALRAFRSSRYRDFRQIRDIMQALLVRPLGKEH
TVSRLLRVMQCLSRIEEGENLDCSFDMEAELTPLESAINVLEMIKTEFTLTDSMVESSRKLVKEAAVIIC
IKNKEFEKASKILKKYMSKDPTTQKLRTDLLNIIREKNLAHPVIQNFSYEVFQQKMLRFLESHLDDTEPY
LLTMAKKALKSESAASSTMREEKHPEPVEKPLREPPSRQPQNPPATIGIRTLKAAFKALSTAQDSEAAFA
KLDQKDLVLANLASPSSPAHKHKRPRKDEHESAAPAEGEGGSDRQPRNSPMTISRLLLEEDSQSTEPSPG
LNSSHEAMSASKPRALNQPHPGEKKPKYEDLLCRSLGAGWRAWLGLVLLP

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC046284
ORF Size 1410 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC046284, AAH46284
RefSeq Size 2539 bp
RefSeq ORF 1412 bp
Locus ID 21750
Gene Summary Binds the telomeric double-stranded 5'-TTAGGG-3' repeat and plays a central role in telomere maintenance and protection against end-to-end fusion of chromosomes. In addition to its telomeric DNA-binding role, required to recruit a number of factors and enzymes required for telomere protection, including the shelterin complex, TERF2IP/RAP1 and DCLRE1B/Apollo. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded 5'-TTAGGG-3' repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Together with DCLRE1B/Apollo, plays a key role in telomeric loop (T loop) formation by generating 3' single-stranded overhang at the leading end telomeres: T loops have been proposed to protect chromosome ends from degradation and repair. Required both to recruit DCLRE1B/Apollo to telomeres and activate the exonuclease activity of DCLRE1B/Apollo. Preferentially binds to positive supercoiled DNA. Together with DCLRE1B/Apollo, required to control the amount of DNA topoisomerase (TOP1, TOP2A and TOP2B) needed for telomere replication during fork passage and prevent aberrant telomere topology. Recruits TERF2IP/RAP1 to telomeres, thereby participating in to repressing homology-directed repair (HDR), which can affect telomere length.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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