Terf2 (BC046284) Mouse Tagged ORF Clone
CAT#: MG207518
- TrueORF®
Terf2 (tGFP-tagged) - Mouse telomeric repeat binding factor 2 (cDNA clone MGC:54756 IMAGE:6439629)
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CNY 3,710.00
CNY 1,000.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | TRF2 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG207518 representing BC046284
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCGGGAGCCGGGACAGCGGGCCCTGCTTCCGGCCCGGGCGTTGTGCGTGACCCGATGGCGTCAC AGCCAAGGAAACGGCCCAGTCGGGAGGGCGGGGAGGGCGGGGAGGGCGAGCGGCGGTCGAACACGATGGC GGGAGGAGGCGGGAGCAGCGATAGCAGCGGGCGGGCGGCGAGCCGACGGGCATCGCGCAGCGGCGGGCGG GCTCGACGGGGGCGACACGAGCCAGGGTTGGGAGGCGCGGCCGAGCGGGGCGCGGGGGAAGCTCGCCTGG AGGAGGCGGTCAACCGCTGGGTGCTCAAGTTCTATTTCCACGAGGCGCTGCGGGCCTTTCGGAGTAGCCG GTACCGGGACTTCAGGCAGATCCGGGACATCATGCAGGCGTTGCTTGTCAGGCCCTTGGGGAAGGAGCAT ACGGTGTCCCGGTTGCTGCGGGTTATGCAGTGTCTGTCGCGCATTGAAGAAGGAGAAAATTTAGACTGTT CCTTTGATATGGAGGCTGAGCTCACACCCTTGGAATCAGCTATCAATGTGCTGGAGATGATTAAAACAGA GTTCACACTGACAGACTCTATGGTTGAATCCAGCAGAAAACTGGTCAAGGAGGCTGCTGTCATTATTTGT ATCAAAAACAAAGAATTTGAAAAGGCTTCAAAGATTTTGAAAAAATACATGTCTAAGGACCCCACAACTC AGAAGCTGAGAACTGATCTCCTGAACATTATCCGGGAAAAGAACTTGGCCCACCCTGTTATCCAGAACTT TTCCTATGAGGTCTTCCAGCAGAAGATGCTGCGTTTCCTAGAGAGCCACCTGGATGACACGGAGCCCTAC CTCCTCACGATGGCTAAAAAAGCTTTGAAATCTGAATCAGCTGCTTCAAGTACAATGAGGGAAGAAAAGC ACCCAGAGCCAGTGGAAAAACCACTTAGAGAGCCTCCAAGCAGACAGCCTCAGAACCCTCCAGCCACCAT CGGGATCAGGACTCTGAAGGCAGCTTTCAAAGCTCTGTCTACTGCACAAGACTCAGAGGCCGCTTTTGCA AAACTGGACCAGAAAGATCTGGTACTTGCTAATCTGGCATCCCCATCATCACCAGCCCACAAACACAAGA GACCCAGGAAAGATGAACATGAAAGCGCAGCTCCTGCTGAGGGTGAGGGAGGCTCAGACCGGCAGCCCAG GAACAGTCCCATGACAATAAGCAGATTGCTGTTGGAGGAGGACAGCCAGAGTACTGAGCCCAGCCCAGGC CTCAACTCCTCCCACGAGGCCATGTCAGCATCCAAGCCCAGAGCTCTCAACCAACCCCACCCGGGGGAGA AGAAGCCCAAGTATGAAGACCTTCTTTGTAGGAGTTTGGGGGCTGGTTGGCGGGCCTGGTTGGGCCTGGT TTTACTTCCA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG207518 representing BC046284
Red=Cloning site Green=Tags(s) MAAGAGTAGPASGPGVVRDPMASQPRKRPSREGGEGGEGERRSNTMAGGGGSSDSSGRAASRRASRSGGR ARRGRHEPGLGGAAERGAGEARLEEAVNRWVLKFYFHEALRAFRSSRYRDFRQIRDIMQALLVRPLGKEH TVSRLLRVMQCLSRIEEGENLDCSFDMEAELTPLESAINVLEMIKTEFTLTDSMVESSRKLVKEAAVIIC IKNKEFEKASKILKKYMSKDPTTQKLRTDLLNIIREKNLAHPVIQNFSYEVFQQKMLRFLESHLDDTEPY LLTMAKKALKSESAASSTMREEKHPEPVEKPLREPPSRQPQNPPATIGIRTLKAAFKALSTAQDSEAAFA KLDQKDLVLANLASPSSPAHKHKRPRKDEHESAAPAEGEGGSDRQPRNSPMTISRLLLEEDSQSTEPSPG LNSSHEAMSASKPRALNQPHPGEKKPKYEDLLCRSLGAGWRAWLGLVLLP TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | BC046284 |
ORF Size | 1410 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | BC046284, AAH46284 |
RefSeq Size | 2539 bp |
RefSeq ORF | 1412 bp |
Locus ID | 21750 |
Gene Summary | Binds the telomeric double-stranded 5'-TTAGGG-3' repeat and plays a central role in telomere maintenance and protection against end-to-end fusion of chromosomes. In addition to its telomeric DNA-binding role, required to recruit a number of factors and enzymes required for telomere protection, including the shelterin complex, TERF2IP/RAP1 and DCLRE1B/Apollo. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded 5'-TTAGGG-3' repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Together with DCLRE1B/Apollo, plays a key role in telomeric loop (T loop) formation by generating 3' single-stranded overhang at the leading end telomeres: T loops have been proposed to protect chromosome ends from degradation and repair. Required both to recruit DCLRE1B/Apollo to telomeres and activate the exonuclease activity of DCLRE1B/Apollo. Preferentially binds to positive supercoiled DNA. Together with DCLRE1B/Apollo, required to control the amount of DNA topoisomerase (TOP1, TOP2A and TOP2B) needed for telomere replication during fork passage and prevent aberrant telomere topology. Recruits TERF2IP/RAP1 to telomeres, thereby participating in to repressing homology-directed repair (HDR), which can affect telomere length.[UniProtKB/Swiss-Prot Function] |
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