Terf2 (BC046284) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	TRF2
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG207518 representing BC046284 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCGGGAGCCGGGACAGCGGGCCCTGCTTCCGGCCCGGGCGTTGTGCGTGACCCGATGGCGTCAC AGCCAAGGAAACGGCCCAGTCGGGAGGGCGGGGAGGGCGGGGAGGGCGAGCGGCGGTCGAACACGATGGC GGGAGGAGGCGGGAGCAGCGATAGCAGCGGGCGGGCGGCGAGCCGACGGGCATCGCGCAGCGGCGGGCGG GCTCGACGGGGGCGACACGAGCCAGGGTTGGGAGGCGCGGCCGAGCGGGGCGCGGGGGAAGCTCGCCTGG AGGAGGCGGTCAACCGCTGGGTGCTCAAGTTCTATTTCCACGAGGCGCTGCGGGCCTTTCGGAGTAGCCG GTACCGGGACTTCAGGCAGATCCGGGACATCATGCAGGCGTTGCTTGTCAGGCCCTTGGGGAAGGAGCAT ACGGTGTCCCGGTTGCTGCGGGTTATGCAGTGTCTGTCGCGCATTGAAGAAGGAGAAAATTTAGACTGTT CCTTTGATATGGAGGCTGAGCTCACACCCTTGGAATCAGCTATCAATGTGCTGGAGATGATTAAAACAGA GTTCACACTGACAGACTCTATGGTTGAATCCAGCAGAAAACTGGTCAAGGAGGCTGCTGTCATTATTTGT ATCAAAAACAAAGAATTTGAAAAGGCTTCAAAGATTTTGAAAAAATACATGTCTAAGGACCCCACAACTC AGAAGCTGAGAACTGATCTCCTGAACATTATCCGGGAAAAGAACTTGGCCCACCCTGTTATCCAGAACTT TTCCTATGAGGTCTTCCAGCAGAAGATGCTGCGTTTCCTAGAGAGCCACCTGGATGACACGGAGCCCTAC CTCCTCACGATGGCTAAAAAAGCTTTGAAATCTGAATCAGCTGCTTCAAGTACAATGAGGGAAGAAAAGC ACCCAGAGCCAGTGGAAAAACCACTTAGAGAGCCTCCAAGCAGACAGCCTCAGAACCCTCCAGCCACCAT CGGGATCAGGACTCTGAAGGCAGCTTTCAAAGCTCTGTCTACTGCACAAGACTCAGAGGCCGCTTTTGCA AAACTGGACCAGAAAGATCTGGTACTTGCTAATCTGGCATCCCCATCATCACCAGCCCACAAACACAAGA GACCCAGGAAAGATGAACATGAAAGCGCAGCTCCTGCTGAGGGTGAGGGAGGCTCAGACCGGCAGCCCAG GAACAGTCCCATGACAATAAGCAGATTGCTGTTGGAGGAGGACAGCCAGAGTACTGAGCCCAGCCCAGGC CTCAACTCCTCCCACGAGGCCATGTCAGCATCCAAGCCCAGAGCTCTCAACCAACCCCACCCGGGGGAGA AGAAGCCCAAGTATGAAGACCTTCTTTGTAGGAGTTTGGGGGCTGGTTGGCGGGCCTGGTTGGGCCTGGT TTTACTTCCA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG207518 representing BC046284 Red=Cloning site Green=Tags(s) MAAGAGTAGPASGPGVVRDPMASQPRKRPSREGGEGGEGERRSNTMAGGGGSSDSSGRAASRRASRSGGR ARRGRHEPGLGGAAERGAGEARLEEAVNRWVLKFYFHEALRAFRSSRYRDFRQIRDIMQALLVRPLGKEH TVSRLLRVMQCLSRIEEGENLDCSFDMEAELTPLESAINVLEMIKTEFTLTDSMVESSRKLVKEAAVIIC IKNKEFEKASKILKKYMSKDPTTQKLRTDLLNIIREKNLAHPVIQNFSYEVFQQKMLRFLESHLDDTEPY LLTMAKKALKSESAASSTMREEKHPEPVEKPLREPPSRQPQNPPATIGIRTLKAAFKALSTAQDSEAAFA KLDQKDLVLANLASPSSPAHKHKRPRKDEHESAAPAEGEGGSDRQPRNSPMTISRLLLEEDSQSTEPSPG LNSSHEAMSASKPRALNQPHPGEKKPKYEDLLCRSLGAGWRAWLGLVLLP TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC046284
ORF Size	1410 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC046284, AAH46284
RefSeq Size	2539 bp
RefSeq ORF	1412 bp
Locus ID	21750
Gene Summary	Binds the telomeric double-stranded 5'-TTAGGG-3' repeat and plays a central role in telomere maintenance and protection against end-to-end fusion of chromosomes. In addition to its telomeric DNA-binding role, required to recruit a number of factors and enzymes required for telomere protection, including the shelterin complex, TERF2IP/RAP1 and DCLRE1B/Apollo. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded 5'-TTAGGG-3' repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Together with DCLRE1B/Apollo, plays a key role in telomeric loop (T loop) formation by generating 3' single-stranded overhang at the leading end telomeres: T loops have been proposed to protect chromosome ends from degradation and repair. Required both to recruit DCLRE1B/Apollo to telomeres and activate the exonuclease activity of DCLRE1B/Apollo. Preferentially binds to positive supercoiled DNA. Together with DCLRE1B/Apollo, required to control the amount of DNA topoisomerase (TOP1, TOP2A and TOP2B) needed for telomere replication during fork passage and prevent aberrant telomere topology. Recruits TERF2IP/RAP1 to telomeres, thereby participating in to repressing homology-directed repair (HDR), which can affect telomere length.[UniProtKB/Swiss-Prot Function]

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