United States
Germany
Japan
United Kingdom
China
Igh (BC018535) Mouse Tagged ORF Clone
CAT＃: MG207534
- TrueORF®
Igh (tGFP-tagged) - Mouse immunoglobulin heavy chain 1a (serum IgG2a) (cDNA clone MGC:6529 IMAGE:2651493)
ORF Plasmid: DDK
"BC018535" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 2,950.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | MGC18977 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG207534 representing BC018535
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGATGGAGCTGGATCTTTCTCTTCCTCCTGTCAGGAACTGCAGGTGTCCTCTCTGAGGTCCAGCTGC AACAGTCTGGACCTGAGTTGGTGAAGACTGGGGCTTCAGTGAAGATGTCCTGCAAGGCTTCTGGATACAC ATTCAGTGACTACTACATGCACTGGGTGAAGCAGAGCCATGGAAAGAGCCTTGAGTGGATTGGATATATT TATCCTAACAATGGTGGTAATGGCTACAACCAGAAGTTCAAGGGCAAGGCCACATTGACTGTAGACAAGT CCTCCAGCACAGCCTACATGGAGCTCCGCAGCCTGACATCTGAGGACTCTGCAGTCTATTACTGTGCAAG AGGATATATAAGTTACTATAGTTACGATCACTACTTTGACTACTGGGGCCAAGGCACCACTATCACAGTC TCCTCAGCCAAAACAACAGCCCCATCGGTCTATCCACTGGCCCCTGTGTGTGGAGATACAACTGGCTCCT CGGTGACTCTAGGATGCCTGGTCAAGGGTTATTTCCCTGAGCCAGTGACCTTGACCTGGAACTCTGGATC CCTGTCCAGTGGTGTGCACACCTTCCCAGCTGTCCTGCAGTCTGACCTCTACACCCTCAGCAGCTCAGTG ACTGTAACCTCGAGCACCTGGCCCAGCCAGTCCATCACCTGCAATGTGGCCCACCCGGCAAGCAGCACCA AGGTGGACAAGAAAATTGAGCCCAGAGGGCCCACAATCAAGCCCTGTCCTCCATGCAAATGCCCAGCACC TAACCTCTTGGGTGGACCATCCGTCTTCATCTTCCCTCCAAAGATCAAGGATGTACTCATGATCTCCCTG AGCCCCATGGTCACATGTGTGGTGGTGGATGTGAGCGAGGATGACCCAGATGTCCAGATCAGCTGGTTCG TGAACAACGTGGAAGTACTCACAGCTCAGACACAAACCCATAGAGAGGATTACAACAGTACTCTCCGGGT GGTCAGTGCCCTCCCCATCCAGCACCAGGACTGGATGAGTGGCAAGGAGTTCAAATGCAAGGTCAACAAC AAAGCCCTCCCAGCGCCCATCGAGAGAACCATCTCAAAACCCAAAGGGTCAGTAAGAGCTCCACAGGTAT ATGTCTTGCCTCCACCAGAAGAAGAGATGACTAAGAAACAGGTCACTCTGACCTGCATGGTCACAGACTT CATGCCTGAAGACATTTACGTGGAGTGGACCAACAACGGGAAAACAGAGCTAAACTACAAGAACACTGAA CCAGTCCTGGACTCTGATGGTTCTTACTTCATGTACAGCAAGCTGAGAGTGGAAAAGAAGAACTGGGTGG AAAGAAATAGCTACTCCTGCTCAGTGGTCCACGAGGGTCTGCACAATCACCACACGACTAAGAGCTTCTC CCGGACTCCGGGTAAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG207534 representing BC018535
Red=Cloning site Green=Tags(s) MGWSWIFLFLLSGTAGVLSEVQLQQSGPELVKTGASVKMSCKASGYTFSDYYMHWVKQSHGKSLEWIGYI YPNNGGNGYNQKFKGKATLTVDKSSSTAYMELRSLTSEDSAVYYCARGYISYYSYDHYFDYWGQGTTITV SSAKTTAPSVYPLAPVCGDTTGSSVTLGCLVKGYFPEPVTLTWNSGSLSSGVHTFPAVLQSDLYTLSSSV TVTSSTWPSQSITCNVAHPASSTKVDKKIEPRGPTIKPCPPCKCPAPNLLGGPSVFIFPPKIKDVLMISL SPMVTCVVVDVSEDDPDVQISWFVNNVEVLTAQTQTHREDYNSTLRVVSALPIQHQDWMSGKEFKCKVNN KALPAPIERTISKPKGSVRAPQVYVLPPPEEEMTKKQVTLTCMVTDFMPEDIYVEWTNNGKTELNYKNTE PVLDSDGSYFMYSKLRVEKKNWVERNSYSCSVVHEGLHNHHTTKSFSRTPGK TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC018535 |
| ORF Size | 1416 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC018535 |
| RefSeq Size | 1593 bp |
| RefSeq ORF | 1418 bp |
| Locus ID | 380793 |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218063 | Igh (untagged) - Mouse immunoglobulin heavy chain 1a (serum IgG2a) (cDNA clone MGC:6529 IMAGE:2651493), (10ug) |
CNY 6,940.00 |
|
| MR207534 | Igh (Myc-DDK-tagged) - Mouse immunoglobulin heavy chain 1a (serum IgG2a) (cDNA clone MGC:6529 IMAGE:2651493) |
CNY 3,656.00 |
|
| MR207534L3 | Lenti ORF clone of Igh (Myc-DDK-tagged) - Mouse immunoglobulin heavy chain 1a (serum IgG2a) (cDNA clone MGC:6529 IMAGE:2651493) |
CNY 4,560.00 |
|
| MR207534L4 | Lenti ORF clone of Igh (mGFP-tagged) - Mouse immunoglobulin heavy chain 1a (serum IgG2a) (cDNA clone MGC:6529 IMAGE:2651493) |
CNY 4,560.00 |
