Irak2 (BC085324) Mouse Tagged ORF Clone

CAT#: MG207686

  • TrueORF®

Irak2 (tGFP-tagged) - Mouse interleukin-1 receptor-associated kinase 2 (cDNA clone MGC:102586 IMAGE:30733777)

ORF Plasmid: DDK tGFP



  "BC085324" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 3,040.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


Rabbit Polyclonal IRAK2 Antibody
    • 100 ug

CNY 5,808.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms 6330415L08Rik; AI649099; IRAK-2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG207686 representing BC085324
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGGGGCCCAGCGGCAGCGTCCGTGCGAGATGGATGCCCCTTGTTCCCTGAAAACTGATGCACCCG
ACTCTCCCCAGTCTAAGTATTGCAGTACTTCCACATCTGCCCCCAAGCAAGAAAGGCTTTTGGGTTTGCC
CGGAGACAGGCTTTTCTGGAGTGAAGCAGATGTCGTCCAAGCAACTGAGGACTTTGACCAAAGCCACCGA
ATCAGCGAGGGCACCTTTGCCGATATCTACCAGGGTCAAAGGAACGGTGTGGCCTTCGCCTTCAAGAAGC
TCAGGGAGGTGGCCGGCTCCAGTCCAGGGTCCATGGACAGATTCCTACAGGCAGAGATGCAGCTCTGCCT
CAGATGCTGCCACGCGAACGTCCTACCTCTGCTGGGTTTCTGCACTGGAAGACAGTTCCACAGCCTCATC
TACCCCTACATGGCAAATGGCTCTCTGCACGACAGACTCTGGGCTCAGGGCAACTCAGACATGCTGCCCT
GGCCACAGCGGGCCAGCATCTGCTCAGGGCTGCTTCTAGCCGTGGAGCACCTGCATAGCCTGGACATCAT
CCACAGCAATGTTAAGAGTGCCAACGTCTTGCTGGACCAGCATTTTAACCCCAAGCTGGCTCATCCTGTG
GCTCACCCTCATCCCGACAATAAAAAGACCAAATATACCGTCATGAGGACTCACCTGTTCCAGGCCTCAG
CTGCATATCTGCCGGAACACTTCATCAGAGTGGGGCAGCTGACCAAGCAGGTGGACATCTTCAGCTGTGG
AATTGTATTGGCCGAGGTCCTTACCGGCATCCCTGCGATGGACAAGGACCGCAGTCCAGTTTACCTGAAG
GATTTGCTTCTCAGTGAGATTCCCAACAGTACTTCTTCAGTCTGCTCCAGGAAGACCAGCATGGGGAAGG
CAGTGGTGAAAGAGATCTGCCAGAGACATGTGGAGAAGAGGGCAGGGCTGCTGCCTGAGGCCTGTGAGGA
AGCCTGGGCCACCGCTGTGAGTGTCTGCCTGCGGAGGCGCAATGCCAGCGTGGAGGAGGCACGAGTCTCC
CTGGCCGGGGTGGAGGAGCAACTCCGAGGGCAGCTGTCCCTTCCCGGGAGCAGGGTTTCTGAGGCCACAG
GCTCATCTTCCAATACCCCGGAGGAAACAGATGACGTTGACAATTCCAGCCTCAGCGTCCCTTCTTTGGT
TATGATGGCATCGTGCCCAGGGGCTGCCTCCTCTCCACTCTTCACGGGACATGGAGCAGCACAGCCATCC
ACCAGTGGGAGACAGGAGGCTGACTCCTCGTCAGAGGCTTGCACAGGCCCACAGACACCCCAGAACGCTA
CAGAGACTTCATGGAAAATTGAGATCAATGAAGCCAAAAGGAGACTGATGGAGAACATCGTACTCTACAA
AGAAGAGAGACTGGACAGCAGCGAGCTCTTTGGACCC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG207686 representing BC085324
Red=Cloning site Green=Tags(s)

MAGAQRQRPCEMDAPCSLKTDAPDSPQSKYCSTSTSAPKQERLLGLPGDRLFWSEADVVQATEDFDQSHR
ISEGTFADIYQGQRNGVAFAFKKLREVAGSSPGSMDRFLQAEMQLCLRCCHANVLPLLGFCTGRQFHSLI
YPYMANGSLHDRLWAQGNSDMLPWPQRASICSGLLLAVEHLHSLDIIHSNVKSANVLLDQHFNPKLAHPV
AHPHPDNKKTKYTVMRTHLFQASAAYLPEHFIRVGQLTKQVDIFSCGIVLAEVLTGIPAMDKDRSPVYLK
DLLLSEIPNSTSSVCSRKTSMGKAVVKEICQRHVEKRAGLLPEACEEAWATAVSVCLRRRNASVEEARVS
LAGVEEQLRGQLSLPGSRVSEATGSSSNTPEETDDVDNSSLSVPSLVMMASCPGAASSPLFTGHGAAQPS
TSGRQEADSSSEACTGPQTPQNATETSWKIEINEAKRRLMENIVLYKEERLDSSELFGP

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC085324
ORF Size 1439 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC085324, AAH85324
RefSeq Size 3049 bp
RefSeq ORF 1439 bp
Locus ID 108960
Gene Summary Binds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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