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Chst7 (NM_021715) Mouse Tagged ORF Clone
CAT＃: MG207747
- TrueORF®
Chst7 (tGFP-tagged) - Mouse carbohydrate (N-acetylglucosamino) sulfotransferase 7 (Chst7)
ORF Plasmid: DDK
"NM_021715" in other vectors (4)
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CNY 4,750.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 2600013M07Rik; C6ST-2; glcNAc6ST-4; Gn6st-4; GST5 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG207747 representing NM_021715
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGGGCCGGCGGCGGCGGCGCCGAGAGTATTGCAAGTTCACGCTGCTCTTGGCGCTGTACACGCTTT TGCTACTTCTTGTCCCCTCTGTACTGGACAGCCACAGCGAGCAGGACAAGGGCAGGAACTGCCCCGGCCT GCAGCGCAGCTTGGGTGTGTGGAGCCTGGAGGCGGCGGCGGCCGGGGAACGTGAGCAGGGCGCTGAGGTG CGGTCCCTGGCCGAAGGAAACCCGGATCGATCCCCCGGGTCCCCCGGCAACCTCAGCGCCGTCGGTGAGG CGGTGACCCAGGAAAAGCAACACATCTATGTGCATGCCACCTGGCGCACCGGCTCGTCCTTCTTGGGCGA ACTCTTCAACCAGCACCCGGACGTTTTCTACTTGTACGAGCCCATGTGGCATCTGTGGCAGGCACTGTAT CCGGGCGACGCGGAGAGCCTGCAGGGCGCACTAAGAGACATGCTGCGCTCCCTCTTCCGCTGTGATTTCT CTGTGCTGCGCCTGTACGCGCAGCCTGGGGACCCTGGGGAGCGAGCACCGGACTCGGCCAACCTCACCAC GGCCATGCTTTTCCGCTGGCGGACCAACAAGGTCATCTGCTCGCCGCCTCTGTGCCCCGCCGCGCCCCGG GCACGCGCGGACGTGGGACTCGTCGAGGACAAAGCCTGCGAAAGTACCTGCCCGCCCGTTTCGCTCCGCG CCCTGGAGGCCGAGTGCCGCAAGTACCCGGTGGTGGTCATCAAAGACGTGCGGCTACTGGACCTGGGAGT GCTGGTCCCTCTGCTGCGTGACCCAGGCCTCAACCTAAAGGTGGTGCAACTCTTCCGAGACCCTCGGGCC GTGCACAACTCGCGCCTCAAGTCGAGGCAGGGACTGCTGCGCGAAAGCATCCAGGTGCTGCGCACGCGCC AGAGGGGCGACCACTTCCACCGGGTGCTGCTGGCGCATGGAGTGGATGCCCGTCCGGGAGGCCAGGCCCG GGCTCTGCCCTCGGCGCCACGCGCTGATTTCTTCTTAACCAGCGCGCTTGAGGTGATCTGTGAAGCGTGG CTTCGCGACCTGCTATTCACCCGCGGCGCGCCCGCCTGGCTGAGGCGTCGCTACCTGCGGCTGCGTTATG AGGACCTGGTGTGGCAGCCCCAAGCCCAGCTGCGCCGCCTGCTGCGCTTCTCTGGGTTGCGGACACTCGC CGCGCTTGATGCCTTCGCATTCAATATGACGCGGGGCTCGGCCTACGGCGCCGATCGTCCCTTCCACTTG TCTGCGCGGGACGCCCGAGAGGCTGTGCACGCCTGGCGCGAACGTCTGAGCCAAGAGCAGGTGCGCCAAG TGGAAACCGCCTGCGCCCCTGCCATGCGTCTGCTTGCCTACCCTCGAAGTGGGGACGAACGCGACAGGAA GACCGTCAGGGAAGGGGAGACACCACTGGAGACCAAGGCCAATTGGGCTGTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG207747 representing NM_021715
Red=Cloning site Green=Tags(s) MKGRRRRRREYCKFTLLLALYTLLLLLVPSVLDSHSEQDKGRNCPGLQRSLGVWSLEAAAAGEREQGAEV RSLAEGNPDRSPGSPGNLSAVGEAVTQEKQHIYVHATWRTGSSFLGELFNQHPDVFYLYEPMWHLWQALY PGDAESLQGALRDMLRSLFRCDFSVLRLYAQPGDPGERAPDSANLTTAMLFRWRTNKVICSPPLCPAAPR ARADVGLVEDKACESTCPPVSLRALEAECRKYPVVVIKDVRLLDLGVLVPLLRDPGLNLKVVQLFRDPRA VHNSRLKSRQGLLRESIQVLRTRQRGDHFHRVLLAHGVDARPGGQARALPSAPRADFFLTSALEVICEAW LRDLLFTRGAPAWLRRRYLRLRYEDLVWQPQAQLRRLLRFSGLRTLAALDAFAFNMTRGSAYGADRPFHL SARDAREAVHAWRERLSQEQVRQVETACAPAMRLLAYPRSGDERDRKTVREGETPLETKANWAV TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_021715 |
| ORF Size | 1452 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_021715.1, NP_068361.1 |
| RefSeq Size | 2248 bp |
| RefSeq ORF | 1455 bp |
| Locus ID | 60322 |
| UniProt ID | Q9EP78 |
| Gene Summary | Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues. Preferentially acts on mannose-linked GlcNAc. Also able to catalyze the transfer of sulfate to position 6 of the N-acetylgalactosamine (GalNAc) residue of chondroitin. Also acts on core 2 mucin-type oligosaccharide and N-acetyllactosamine oligomer with a lower efficiency. Has weak or no activity toward keratan sulfate and oligosaccharides containing the Galbeta1-4GlcNAc. Catalyzes 6-O-sulfation of beta-benzyl GlcNAc but not alpha- or beta-benzyl GalNAc.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC202940 | Chst7 (untagged) - Mouse carbohydrate (N-acetylglucosamino) sulfotransferase 7 (Chst7), (10ug) |
CNY 3,656.00 |
|
| MR207747 | Chst7 (Myc-DDK-tagged) - Mouse carbohydrate (N-acetylglucosamino) sulfotransferase 7 (Chst7) |
CNY 3,656.00 |
|
| MR207747L3 | Lenti ORF clone of Chst7 (Myc-DDK-tagged) - Mouse carbohydrate (N-acetylglucosamino) sulfotransferase 7 (Chst7) |
CNY 6,180.00 |
|
| MR207747L4 | Lenti ORF clone of Chst7 (mGFP-tagged) - Mouse carbohydrate (N-acetylglucosamino) sulfotransferase 7 (Chst7) |
CNY 6,180.00 |
