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Cdc14b (NM_172587) Mouse Tagged ORF Clone
CAT＃: MG207764
- TrueORF®
Cdc14b (tGFP-tagged) - Mouse CDC14 cell division cycle 14 homolog B (S. cerevisiae) (Cdc14b)
ORF Plasmid: DDK
"NM_172587" in other vectors (4)
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CNY 3,710.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 2810432N10Rik; A530086E13Rik; AA472821; Cdc14B1; CDC14B3 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG207764 representing NM_172587
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGCGTAAGAGCGAGCGGCGCTCGGCTTGGGCCACCGCGCCCCCCTGCTCACGCCGCAGCTCGTCCT CCTCTCCCGGCGTGAAGAAGAGCCGCAGCTCCACGCCGCAGGAGCTGCACCGCCTGGAGCAGCAGGACGA CCTGTACCTGGACATCACGGATCGCCTTTGTTTTGCCATTCTCTACAGCAGACCAAAGAGTGCAACAAAC GAACATTATTTCAGCATAGATAACGAGCTAGAGTATGAGAACTTCTATGCGGATTTTGGACCACTCAATC TGGCCATGGTTTACAGATATTGTTGCAAGATAAATAAGAAGCTCAAGTCCATTACAATGCTGAGGAAGAA AATTATCCATTTTACTGGCACTGATCAGAGAAAGCAAGCGAATGCCGCCTTCCTTGTCGGGTGTTATATG GTTATATATTTGGGGAGGACACCAGAAGACGCGTATAGAACATTAATCTTCGGAGATACAGCCTATATTC CTTTCAGAGATGCTGCCTATGGAAGCTGCAGTTTCTATATTACACTTCTCGACTGTTTTCACGCAGTAAA GAAGGCAATGCAGTATGGCTTCTTTAATTTCAACTCATTTAACCTTGACGAATATGAACACTATGAAAAA GCAGAAAATGGAGATTTCAACTGGATAATACCAGAGCGATTTCTTGCCTTCTGTGGGCCCCATTCAAGAT CCAGGCTGGAAAGTGGTTACCACCAACATTCTCCGGAGACTTATATTCCATATTTTAAGAATCACAATGT AACTACCATTATTCGTCTGAATAAAAGGATGTATGATGCTAAGCGCTTTACGGATGCTGGATTCGATCAC CATGATCTTTTCTTTCCGGATGGCAGCACCCCTGCTGAGTCAATTGTCCAAGAATTTCTGGATATTTGTG AAAATGTCAAGGGTGCCATTGCTGTGCATTGCAAAGCTGGCCTTGGTCGAACAGGCACTCTGATAGGCTG CTACCTCATGAAGCATTACAGGATGACAGCAGCTGAGAGCATTGCCTGGCTCAGAATCTGTAGACCCGGC TCAGTAATTGGGCCCCAGCAACAGTTTCTGGTCATGAAACAATCAAGCCTCTGGCTAGAAGGCGACTATT TCCGTCAAAAGTTAAGAGGGCAGGAGAATGGCCCCCTCAGAGAAGCCTTCTCCAAACACCTTTCGGATGC TGATGACCTGTCCTTAAATGGGCTTGAGAATCAAGACAATCAAGAGCCTGAGCCGTATAGTGATGATGAT GAAGTCAGTGGAATGACACAAGGAGACAGACTTCGGGCCCTGAAAAGCCGGAGACAGCCGAAAGCCAGCG CTATCCCCCTCACATGTCCCCTAGCTGTGCTGACCTCTGCACTGTGTAGTGTTGCCATCTGGTGGATTGT GTGTGACTACATTCTTCCCACCCTGCTGTTCTGTCTTGATGGCTTCAGAACACAG AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG207764 representing NM_172587
Red=Cloning site Green=Tags(s) MKRKSERRSAWATAPPCSRRSSSSSPGVKKSRSSTPQELHRLEQQDDLYLDITDRLCFAILYSRPKSATN EHYFSIDNELEYENFYADFGPLNLAMVYRYCCKINKKLKSITMLRKKIIHFTGTDQRKQANAAFLVGCYM VIYLGRTPEDAYRTLIFGDTAYIPFRDAAYGSCSFYITLLDCFHAVKKAMQYGFFNFNSFNLDEYEHYEK AENGDFNWIIPERFLAFCGPHSRSRLESGYHQHSPETYIPYFKNHNVTTIIRLNKRMYDAKRFTDAGFDH HDLFFPDGSTPAESIVQEFLDICENVKGAIAVHCKAGLGRTGTLIGCYLMKHYRMTAAESIAWLRICRPG SVIGPQQQFLVMKQSSLWLEGDYFRQKLRGQENGPLREAFSKHLSDADDLSLNGLENQDNQEPEPYSDDD EVSGMTQGDRLRALKSRRQPKASAIPLTCPLAVLTSALCSVAIWWIVCDYILPTLLFCLDGFRTQ SGPTRTRRLE - GFP Tag - V |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_172587 |
| ORF Size | 1455 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_172587.3, NP_766175.3 |
| RefSeq Size | 5859 bp |
| RefSeq ORF | 1458 bp |
| Locus ID | 218294 |
| UniProt ID | Q6PFY9 |
| Gene Summary | Dual-specificity phosphatase involved in DNA damage response. Essential regulator of the G2 DNA damage checkpoint: following DNA damage, translocates to the nucleus and dephosphorylates FZR1/CDH1, a key activator of the anaphase promoting complex/cyclosome (APC/C). Dephosphorylates SIRT2 around early anaphase. Dephosphorylation of FZR1/CDH1 activates the APC/C, leading to the ubiquitination of PLK1, preventing entry into mitosis. Preferentially dephosphorylates proteins modified by proline-directed kinases (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC216713 | Cdc14b (untagged) - Mouse CDC14 cell division cycle 14 homolog B (S. cerevisiae) (Cdc14b), transcript variant 1, (10ug) |
CNY 6,270.00 |
|
| MR207764 | Cdc14b (Myc-DDK-tagged) - Mouse CDC14 cell division cycle 14 homolog B (S. cerevisiae) (Cdc14b), transcript variant 1 |
CNY 3,656.00 |
|
| MR207764L3 | Lenti ORF clone of Cdc14b (Myc-DDK-tagged) - Mouse CDC14 cell division cycle 14 homolog B (S. cerevisiae) (Cdc14b), transcript variant 1 |
CNY 5,230.00 |
|
| MR207764L4 | Lenti ORF clone of Cdc14b (mGFP-tagged) - Mouse CDC14 cell division cycle 14 homolog B (S. cerevisiae) (Cdc14b), transcript variant 1 |
CNY 5,230.00 |
