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Prkcd (BC051416) Mouse Tagged ORF Clone
CAT＃: MG207804
- TrueORF®
Prkcd (tGFP-tagged) - Mouse protein kinase C, delta (cDNA clone MGC:58920 IMAGE:6515302)
ORF Plasmid: DDK
"BC051416" in other vectors (4)
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CNY 5,320.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | PKC[d], PKCdelta |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG207804 representing BC051416
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCACCCTTCCTGCGCATCTCCTTCAATTCCTATGAGCTGGGCTCCCTGCAAGTTGAGGACGAAGCAA GCCAGCCTTTCTGTGCTGTGAAGATGAAGGAGGCACTCAGCACAGAGCGAGGGAAGACACTGGTACAGAA GAAGCCCACCATGTATCCTGAGTGGAAGACAACGTTCGACGCCCACATCTATGAAGGCCGTGTTATCCAG ATTGTGCTGATGCGGGCAGCTGAAGACCCCGTGTCTGAGGTCACGGTGGGCGTGTCAGTACTGGCTGAGC GCTGCAAGAAGAACAACGGCAAGGCTGAGTTCTGGCTGGATCTGCAGCCTCAGGCCAAGGTGCTGATGTG TGTGCAGTATTTCCTGGAGGATGGGGATTGCAAACAGTCTATGCGTAGTGAGGAGGAGGCAAAGTTTCCA ACCATGAACCGTCGTGGAGCCATTAAACAGGCCAAGATCCACTACATCAAGAACCACGAGTTTATCGCCA CCTTCTTCGGGCAGCCCACCTTCTGTTCTGTGTGCAAAGAGTTTGTCTGGGGCCTCAACAAGCAAGGCTA CAAATGCAGGCAATGCAACGCTGCCATCCACAAGAAATGCATTGACAAGATTATCGGCCGCTGCACTGGC ACTGCCACCAATAGCCGGGACACCATCTTCCAGAAAGAACGCTTCAACATCGACATGCCTCACCGATTCA AGGTTTATAACTACATGAGCCCCACCTTCTGTGACCACTGTGGCAGTTTGCTCTGGGGACTGGTGAAGCA GGGATTAAAGTGTGAAGATTGTGGCATGAATGTGCACCACAAATGCCGGGAGAAGGTGGCCAACCTGTGT GGTATCAACCAAAAGCTCTTGGCTGAGGCCTTGAACCAAGTGACCCAGAGATCTTCCCGGAAGCTGGACA CAACAGAGTCTGTCGGAATATACCAGGGATTTGAGAAGAAGCCAGAAGTCTCTGGGAGTGACATCCTAGA CAACAACGGGACCTATGGCAAGATCTGGGAGGGGAGCACCCGGTGCACCCTTGAGAACTTCACCTTCCAA AAAGTACTTGGCAAAGGCAGCTTTGGCAAGGTGCTGCTGGCAGAGCTGAAGGGCAAAGACAAGTACTTTG CAATCAAGTGTCTGAAGAAGGACGTGGTGTTGATTGACGATGATGTAGAGTGTACCATGGTGGAGAAGCG GGTGCTGGCGCTCGCCTGGGAGAGTCCCTTCCTCACCCACCTCATCTGTACCTTCCAGACCAAGGACCAC CTGTTCTTCGTGATGGAGTTTCTCAATGGGGGTGACCTGATGTTCCACATTCAGGACAAAGGCCGCTTCG AACTCTACCGGGCTACGTTTTATGCAGCTGAGATCATCTGCGGACTGCAGTTTCTACACAGCAAAGGCAT TATTTACAGGTATTGTTGGTGCTGGGGGCCGTGGGGAGGGACTTCTGGGAAGGCTGTTGCT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG207804 representing BC051416
Red=Cloning site Green=Tags(s) MAPFLRISFNSYELGSLQVEDEASQPFCAVKMKEALSTERGKTLVQKKPTMYPEWKTTFDAHIYEGRVIQ IVLMRAAEDPVSEVTVGVSVLAERCKKNNGKAEFWLDLQPQAKVLMCVQYFLEDGDCKQSMRSEEEAKFP TMNRRGAIKQAKIHYIKNHEFIATFFGQPTFCSVCKEFVWGLNKQGYKCRQCNAAIHKKCIDKIIGRCTG TATNSRDTIFQKERFNIDMPHRFKVYNYMSPTFCDHCGSLLWGLVKQGLKCEDCGMNVHHKCREKVANLC GINQKLLAEALNQVTQRSSRKLDTTESVGIYQGFEKKPEVSGSDILDNNGTYGKIWEGSTRCTLENFTFQ KVLGKGSFGKVLLAELKGKDKYFAIKCLKKDVVLIDDDVECTMVEKRVLALAWESPFLTHLICTFQTKDH LFFVMEFLNGGDLMFHIQDKGRFELYRATFYAAEIICGLQFLHSKGIIYRYCWCWGPWGGTSGKAVA TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC051416 |
| ORF Size | 1461 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC051416.1 |
| RefSeq Size | 2517 bp |
| RefSeq ORF | 1463 bp |
| Locus ID | 18753 |
| Gene Summary | Calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that plays contrasting roles in cell death and cell survival by functioning as a pro-apoptotic protein during DNA damage-induced apoptosis, but acting as an anti-apoptotic protein during cytokine receptor-initiated cell death, is involved in tumor suppression, is required for oxygen radical production by NADPH oxidase and acts as positive or negative regulator in platelet functional responses. Negatively regulates B cell proliferation and also has an important function in self-antigen induced B cell tolerance induction. Upon DNA damage, activates the promoter of the death-promoting transcription factor BCLAF1/Btf to trigger BCLAF1-mediated p53/TP53 gene transcription and apoptosis. In response to oxidative stress, interact with and activate CHUK/IKKA in the nucleus, causing the phosphorylation of p53/TP53. In the case of ER stress or DNA damage-induced apoptosis, can form a complex with the tyrosine-protein kinase ABL1 which trigger apoptosis independently of p53/TP53. In cytosol can trigger apoptosis by activating MAPK11 or MAPK14, inhibiting AKT1 and decreasing the level of X-linked inhibitor of apoptosis protein (XIAP), whereas in nucleus induces apoptosis via the activation of MAPK8 or MAPK9. Upon ionizing radiation treatment, is required for the activation of the apoptosis regulators BAX and BAK, which trigger the mitochondrial cell death pathway. Can phosphorylate MCL1 and target it for degradation which is sufficient to trigger for BAX activation and apoptosis. Is required for the control of cell cycle progression both at G1/S and G2/M phases. Mediates phorbol 12-myristate 13-acetate (PMA)-induced inhibition of cell cycle progression at G1/S phase by up-regulating the CDK inhibitor CDKN1A/p21 and inhibiting the cyclin CCNA2 promoter activity. In response to UV irradiation can phosphorylate CDK1, which is important for the G2/M DNA damage checkpoint activation. Can protect glioma cells from the apoptosis induced by TNFSF10/TRAIL, probably by inducing increased phosphorylation and subsequent activation of AKT1. Can also act as tumor suppressor upon mitogenic stimulation with PMA or TPA. In N-formyl-methionyl-leucyl-phenylalanine (fMLP)-treated cells, is required for NCF1 (p47-phox) phosphorylation and activation of NADPH oxidase activity, and regulates TNF-elicited superoxide anion production in neutrophils, by direct phosphorylation and activation of NCF1 or indirectly through MAPK1/3 (ERK1/2) signaling pathways. May also play a role in the regulation of NADPH oxidase activity in eosinophil after stimulation with IL5, leukotriene B4 or PMA. In collagen-induced platelet aggregation, acts a negative regulator of filopodia formation and actin polymerization by interacting with and negatively regulating VASP phosphorylation. Downstream of PAR1, PAR4 and CD36/GP4 receptors, regulates differentially platelet dense granule secretion; acts as a positive regulator in PAR-mediated granule secretion, whereas it negatively regulates CD36/GP4-mediated granule release. Phosphorylates MUC1 in the C-terminal and regulates the interaction between MUC1 and beta-catenin. The catalytic subunit phosphorylates 14-3-3 proteins (YWHAB, YWHAZ and YWHAH) in a sphingosine-dependent fashion. Phosphorylates ELAVL1 in response to angiotensin-2 treatment (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218221 | Prkcd (untagged) - Mouse protein kinase C, delta (cDNA clone MGC:58920 IMAGE:6515302), (10ug) |
CNY 6,940.00 |
|
| MR207804 | Prkcd (Myc-DDK-tagged) - Mouse protein kinase C, delta (cDNA clone MGC:58920 IMAGE:6515302) |
CNY 4,850.00 |
|
| MR207804L3 | Lenti ORF clone of Prkcd (Myc-DDK-tagged) - Mouse protein kinase C, delta (cDNA clone MGC:58920 IMAGE:6515302) |
CNY 6,750.00 |
|
| MR207804L4 | Lenti ORF clone of Prkcd (mGFP-tagged) - Mouse protein kinase C, delta (cDNA clone MGC:58920 IMAGE:6515302) |
CNY 6,750.00 |
