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Nampt (NM_021524) Mouse Tagged ORF Clone
CAT＃: MG207867
- TrueORF®
Nampt (tGFP-tagged) - Mouse pre-B-cell colony-enhancing factor 1 (Pbef1)
ORF Plasmid: DDK
"NM_021524" in other vectors (4)
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CNY 5,256.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 1110035O14Rik; AI314458; AI480535; NAmPRTase; Pbef; Pbef1; Visfatin |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG207867 representing NM_021524
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATGCTGCGGCAGAAGCCGAGTTCAACATCCTGCTGGCCACCGACTCGTACAAGGTTACTCACTATA AACAATACCCACCCAACACAAGCAAAGTTTATTCCTACTTTGAATGCCGTGAAAAGAAGACAGAAAACTC CAAAGTAAGGAAGGTGAAATACGAGGAAACAGTATTTTATGGGTTGCAGTACATTCTTAATAAGTACTTA AAAGGTAAAGTAGTGACCAAAGAGAAAATCCAGGAGGCCAAAGAAGTGTACAGAGAACATTTCCAAGATG ATGTCTTTAACGAAAGAGGATGGAACTACATCCTTGAGAAATACGATGGTCATCTCCCAATTGAAGTAAA GGCTGTTCCCGAGGGCTCTGTCATCCCCAGAGGGAACGTGCTGTTCACAGTGGAAAACACAGACCCAGAG TGCTACTGGCTTACCAATTGGATTGAGACTATTCTTGTTCAGTCCTGGTATCCAATTACAGTGGCCACAA ATTCCAGAGAACAGAAGAAAATACTGGCCAAATATTTGTTAGAAACCTCTGGTAACTTAGATGGTCTGGA ATACAAGTTACATGACTTTGGTTACAGAGGAGTCTCTTCGCAAGAGACTGCTGGCATAGGGGCATCTGCT CATTTGGTTAACTTTAAAGGAACAGATACTGTGGCGGGAATTGCTCTAATTAAAAAATACTATGGGACAA AAGATCCTGTTCCAGGCTATTCTGTTCCAGCAGCAGAGCACAGTACCATAACGGCTTGGGGGAAAGACCA TGAGAAAGATGCTTTTGAACACATAGTAACACAGTTCTCATCAGTGCCTGTGTCTGTGGTCAGCGATAGC TATGACATTTATAATGCGTGTGAGAAAATATGGGGTGAAGACCTGAGACATCTGATAGTATCGAGAAGTA CAGAGGCACCACTAATCATCAGACCTGACTCTGGAAATCCTCTTGACACTGTATTGAAGGTCTTAGATAT TTTAGGCAAGAAGTTTCCTGTTACTGAGAACTCAAAAGGCTACAAGTTGCTGCCACCTTATCTTAGAGTC ATTCAAGGAGATGGCGTGGATATCAATACTTTACAAGAGATTGTAGAGGGAATGAAACAAAAGAAGTGGA GTATCGAGAATGTCTCCTTCGGTTCTGGTGGCGCTTTGCTACAGAAGTTAACCCGAGACCTCTTGAATTG CTCCTTCAAGTGCAGCTATGTTGTAACCAATGGCCTTGGGGTTAATGTGTTTAAGGACCCAGTTGCTGAT CCCAACAAAAGGTCAAAAAAGGGCCGGTTATCTTTACATAGGACACCAGCGGGGAACTTTGTTACACTTG AAGAAGGAAAAGGAGACCTTGAGGAATATGGCCATGATCTTCTCCATACGGTTTTCAAGAATGGGAAGGT GACAAAAAGCTACTCATTTGATGAAGTCAGAAAAAATGCACAGCTGAACATCGAGCAGGACGTGGCACCT CAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG207867 representing NM_021524
Red=Cloning site Green=Tags(s) MNAAAEAEFNILLATDSYKVTHYKQYPPNTSKVYSYFECREKKTENSKVRKVKYEETVFYGLQYILNKYL KGKVVTKEKIQEAKEVYREHFQDDVFNERGWNYILEKYDGHLPIEVKAVPEGSVIPRGNVLFTVENTDPE CYWLTNWIETILVQSWYPITVATNSREQKKILAKYLLETSGNLDGLEYKLHDFGYRGVSSQETAGIGASA HLVNFKGTDTVAGIALIKKYYGTKDPVPGYSVPAAEHSTITAWGKDHEKDAFEHIVTQFSSVPVSVVSDS YDIYNACEKIWGEDLRHLIVSRSTEAPLIIRPDSGNPLDTVLKVLDILGKKFPVTENSKGYKLLPPYLRV IQGDGVDINTLQEIVEGMKQKKWSIENVSFGSGGALLQKLTRDLLNCSFKCSYVVTNGLGVNVFKDPVAD PNKRSKKGRLSLHRTPAGNFVTLEEGKGDLEEYGHDLLHTVFKNGKVTKSYSFDEVRKNAQLNIEQDVAP H TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_021524 |
| ORF Size | 1473 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_021524.1, NP_067499.1 |
| RefSeq Size | 2664 bp |
| RefSeq ORF | 1476 bp |
| Locus ID | 59027 |
| UniProt ID | Q99KQ4 |
| Gene Summary | The secreted form behaves both as a cytokine with immunomodulating properties and an adipokine with anti-diabetic properties, it has no enzymatic activity, partly because of lack of activation by ATP, which has a low level in extracellular space and plasma (By similarity). Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway. Plays a role in the modulation of circadian clock function. NAMPT-dependent oscillatory production of NAD regulates oscillation of clock target gene expression by releasing the core clock component: CLOCK-ARNTL/BMAL1 heterodimer from NAD-dependent SIRT1-mediated suppression.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC200248 | Nampt (untagged) - Mouse nicotinamide phosphoribosyltransferase (Nampt), (10ug) |
CNY 2,000.00 |
|
| MR207867 | Nampt (Myc-DDK-tagged) - Mouse nicotinamide phosphoribosyltransferase (Nampt) |
CNY 3,656.00 |
|
| MR207867L3 | Lenti ORF clone of Nampt (Myc-DDK-tagged) - Mouse nicotinamide phosphoribosyltransferase (Nampt) |
CNY 5,230.00 |
|
| MR207867L4 | Lenti ORF clone of Nampt (mGFP-tagged) - Mouse nicotinamide phosphoribosyltransferase (Nampt) |
CNY 5,230.00 |
