Trim39 (BC031540) Mouse Tagged ORF Clone

CAT#: MG207964

  • TrueORF®

Trim39 (tGFP-tagged) - Mouse tripartite motif protein 39 (cDNA clone MGC:19239 IMAGE:3666785)

ORF Plasmid: DDK tGFP



  "BC031540" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 3,140.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms tfp, RBCC-B30.2, mKIAA4179
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG207964 representing BC031540
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGAGACAAGTCTGTTAGAGGCTGGAGCCTCTGCAGCCTCCACAGCCGCTGCTCTGGAGAACCTGC
AGGTGGAAGCAAGCTGTTCTGTATGCCTAGAGTATCTGAAGGAGCCAGTTATTATTGAATGTGGGCACAA
CTTCTGCAAAGCGTGCATTACCCGCTGGTGGGAAGACCTAGAGCGGGACTTCCCTTGCCCTGTCTGTCGG
AAGACATCCCGATACCGGAGTCTCCGGCCTAACCGACAGCTAGGCAGTATGGTGGAAATCGCCAAGCAGC
TCCAGACGGTCAAGCGGAAGATCAGAGATGAAAGCCTCTGCTCCCAGCACCACGAACCTCTTAGCCTTTT
CTGCTATGAAGACCAGGAGGCTGTCTGTCTAATATGTGCAATTTCTCATACCCACCGGCCCCACACCGTC
GTGCCGATGGATGATGCTACACAGGAATACAAGGAGAAACTTCAGAAGTGCCTGGAGCCCCTGGAACAGA
AGCTGCAGGAGATCACCTGCTGCAAAGCCTCCGAAGAGAAGAAGCCGGGGGAGCTCAAGAGACTGGTGGA
GAGCCGCCGGCAGCAGATCTTAAAGGAGTTTGAGGAGCTGCACAGGCGACTGGACGAAGAGCAGCAGACA
CTGCTTTCACGACTGGAGGAAGAAGAACAGGACATTCTACAGCGACTTCGAGAAAATGCTGCTCACCTTG
GGGACAGGCGCCGGGACCTTGCCCACTTAGCTGCTGAGGTGGAGGGCAAGTGCTTACAGTCAGGCTTCGA
GATGCTTAAGGATGTGAAAAGTACCCTAGAAAAATGTGAGAAGGTGAAGACCATGGAGGTGACTTCAGTA
TCCATAGAGCTGGAAAAGAACTTCAGCAATTTCCCCCGGCAGTACTTTGCCCTAAGGAAAATTCTTAAAC
AGCTAATTGCTCCACTCTGGCTTCTTCCGCCAGCGGATGTGACTCTGGACCCCGAGACGGCTCACCCTAA
CCTCGTCCTGTCAGAGGATCGGAAAAGCGTCAAGTTTGTGGAGACAAGACTCCGAGACCTCCCTGACACA
CCGCAGCGTTTCACTTTCTACCCTTGTGTCTTAGCTACTGAAGGCTTCACCTCAGGTCGACACTACTGGG
AGGTGGAGGTGGGTGACAAGACCCACTGGGCAGTAGGAGTATGCCGGGACTCTGTGAGCAGAAAGGGCGA
ACTGACTCCACTTCCTGAGACTGGCTACTGGCGAGTGCGGCTGTGGAATGGGGACAAGTATGCAGCCACC
ACCACGCCTTTTACCCCTTTGCACATCAAGGTGAAGCCCAAGCGGGTAGGCATATTCCTAGACTATGAGG
CTGGCACACTGTCCTTTTACAACGTCACAGACCGTTCACATATCTACACCTTCACTGATACTTTTACTGA
AAAACTTTGGCCCCTCTTCTACCCAGGGATCCGGGCCGGTCGGAAGAATGCTGCACCACTTACCATTAGG
CCCCCAACAGATTGGGAA


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG207964 representing BC031540
Red=Cloning site Green=Tags(s)

MAETSLLEAGASAASTAAALENLQVEASCSVCLEYLKEPVIIECGHNFCKACITRWWEDLERDFPCPVCR
KTSRYRSLRPNRQLGSMVEIAKQLQTVKRKIRDESLCSQHHEPLSLFCYEDQEAVCLICAISHTHRPHTV
VPMDDATQEYKEKLQKCLEPLEQKLQEITCCKASEEKKPGELKRLVESRRQQILKEFEELHRRLDEEQQT
LLSRLEEEEQDILQRLRENAAHLGDRRRDLAHLAAEVEGKCLQSGFEMLKDVKSTLEKCEKVKTMEVTSV
SIELEKNFSNFPRQYFALRKILKQLIAPLWLLPPADVTLDPETAHPNLVLSEDRKSVKFVETRLRDLPDT
PQRFTFYPCVLATEGFTSGRHYWEVEVGDKTHWAVGVCRDSVSRKGELTPLPETGYWRVRLWNGDKYAAT
TTPFTPLHIKVKPKRVGIFLDYEAGTLSFYNVTDRSHIYTFTDTFTEKLWPLFYPGIRAGRKNAAPLTIR
PPTDWE

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC031540
ORF Size 1490 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC031540, AAH31540
RefSeq Size 2948 bp
RefSeq ORF 1490 bp
Locus ID 79263
Gene Summary E3 ubiquitin-protein ligase. May facilitate apoptosis by inhibiting APC/C-Cdh1-mediated poly-ubiquitination and subsequent proteasome-mediated degradation of the pro-apoptotic protein MOAP1.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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