Glrb (BC037605) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	spastic, Glyrb
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG207969 representing BC037605 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGTTTTCGTTGGCAATCTCCTTTTTTATTTTAATGTCCTTGTTGTTTGAAGACGCTTGTGCTAAAG AAAAGTCTTCCAAGAAAGGGAAGGGGAAAAAGAAGCAGTACTTGTGCCCATCTCAGCAGTCACCCGAGGA CCTGGCCCGTGTGCCCCCCAACTCCACCAGCAATATCTTGAACAGGCTGCTGGTCAGTTATGATCCCAGG ATCAGACCAAACTTCAAAGGCATTCCTGTTGATGTAGTAGTCAACATTTTTATTAATAGTTTTGGATCCA TTCAAGAGACAACAATGGACTATAGAGTTAACATTTTCTTGAGACAGAAATGGAATGACCCCAGACTCAA GCTACCTAGTGACTTCAGAGGCTCAGATGCACTGACAGTTGACCCCACCATGTATAAGTGCTTGTGGAAA CCTGACTTATTCTTTGCAAATGAAAAAAGTGCCAATTTTCATGATGTGACCCAAGAAAATATCCTGTTGT TTATCTTTCGGGATGGAGACGTCCTTGTGAGCATGAGGTTGTCTATTACACTTTCATGTCCTCTAGACTT AACTCTGTTTCCCATGGACACACAACGCTGCAAAATGCAACTTGAGAGCTTTGGATATACAACCGATGAT TTAAGATTCATCTGGCAGTCAGGAGATCCTGTTCAGTTGGAAAAAATTGCTTTACCTCAATTTGATATTA AAAAGGAGGATATCGAATATGGCAACTGTACAAAATACTATAAAGGCACTGGTTACTACACTTGTGTGGA GGTCATCTTCACCCTGAGGAGACAGGTTGGGTTCTACATGATGGGCGTATATGCACCAACCTTGCTGATT GTGGTTCTCTCCTGGCTCTCTTTCTGGATCAACCCTGATGCTAGTGCTGCCAGAGTACCTCTGGGCATCT TCTCCGTGCTCAGTTTGGCCTCAGAGTGCACCACCCTCGCAGCCGAGCTTCCTAAAGTGTCTTATGTGAA GGCGCTGGATGTGTGGCTCATTGCCTGCCTGCTCTTCGGGTTTGCCTCCCTCGTGGAGTACGCTGTGGTC CAGGTGATGCTGAACAATCCCAAAAGGGTTGAAGCCGAGAAGGCCCGAATAGCTAAGGCTGAGCAAGCAG ATGGGAAAGGTGGAAACGCAGCTAAGAAGAATACTGTAAACGGCACGGGGACCCCTGTTCATATCAGCAC TTTGCAGGTTGGTGAGACCAGATGCAAAAAAGTTTGTACTTCCAAGTCTGATTTGAGATCCAATGACTTC AGCATTGTTGGAAGCTTACCAAGAGATTTTGAATTATCCAATTATGACTGCTATGGGAAACCCATCGAAG TCAACAATGGACTTGGGAAACCACAGGCAAAGAACAAGAAGCCTCCGCCTGCCAAGCCTGTCATCCCAAC AGCAGCCAAGCGCATCGACCTTTATGCAAGAGCATTATTTCCTTTCTGCTTCTTGTTCTTCAATGTTATA TATTGGTCTATATATTTA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG207969 representing BC037605 Red=Cloning site Green=Tags(s) MKFSLAISFFILMSLLFEDACAKEKSSKKGKGKKKQYLCPSQQSPEDLARVPPNSTSNILNRLLVSYDPR IRPNFKGIPVDVVVNIFINSFGSIQETTMDYRVNIFLRQKWNDPRLKLPSDFRGSDALTVDPTMYKCLWK PDLFFANEKSANFHDVTQENILLFIFRDGDVLVSMRLSITLSCPLDLTLFPMDTQRCKMQLESFGYTTDD LRFIWQSGDPVQLEKIALPQFDIKKEDIEYGNCTKYYKGTGYYTCVEVIFTLRRQVGFYMMGVYAPTLLI VVLSWLSFWINPDASAARVPLGIFSVLSLASECTTLAAELPKVSYVKALDVWLIACLLFGFASLVEYAVV QVMLNNPKRVEAEKARIAKAEQADGKGGNAAKKNTVNGTGTPVHISTLQVGETRCKKVCTSKSDLRSNDF SIVGSLPRDFELSNYDCYGKPIEVNNGLGKPQAKNKKPPPAKPVIPTAAKRIDLYARALFPFCFLFFNVI YWSIYL TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC037605
ORF Size	1490 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC037605, AAH37605
RefSeq Size	3008 bp
RefSeq ORF	1490 bp
Locus ID	14658
Gene Summary	This gene encodes the beta subunit of the glycine receptor, which is a pentamer composed of alpha and beta subunits. The receptor functions as a neurotransmitter-gated ion channel, which produces hyperpolarization via increased chloride conductance due to the binding of glycine to the receptor. This gene is transcribed throughout the central nervous system of neonatal and adult mice. In humans, mutations in this gene cause startle disease, also known as hereditary hyperekplexia or congenital stiff-person syndrome, a disease characterized by muscular rigidity. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2016]

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