Glrb (BC037605) Mouse Tagged ORF Clone

CAT#: MG207969

  • TrueORF®

Glrb (tGFP-tagged) - Mouse glycine receptor, beta subunit (cDNA clone MGC:46752 IMAGE:5361798)



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Mouse Protein / Lysate Request

CNY 4,850.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms spastic, Glyrb
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG207969 representing BC037605
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGTTTTCGTTGGCAATCTCCTTTTTTATTTTAATGTCCTTGTTGTTTGAAGACGCTTGTGCTAAAG
AAAAGTCTTCCAAGAAAGGGAAGGGGAAAAAGAAGCAGTACTTGTGCCCATCTCAGCAGTCACCCGAGGA
CCTGGCCCGTGTGCCCCCCAACTCCACCAGCAATATCTTGAACAGGCTGCTGGTCAGTTATGATCCCAGG
ATCAGACCAAACTTCAAAGGCATTCCTGTTGATGTAGTAGTCAACATTTTTATTAATAGTTTTGGATCCA
TTCAAGAGACAACAATGGACTATAGAGTTAACATTTTCTTGAGACAGAAATGGAATGACCCCAGACTCAA
GCTACCTAGTGACTTCAGAGGCTCAGATGCACTGACAGTTGACCCCACCATGTATAAGTGCTTGTGGAAA
CCTGACTTATTCTTTGCAAATGAAAAAAGTGCCAATTTTCATGATGTGACCCAAGAAAATATCCTGTTGT
TTATCTTTCGGGATGGAGACGTCCTTGTGAGCATGAGGTTGTCTATTACACTTTCATGTCCTCTAGACTT
AACTCTGTTTCCCATGGACACACAACGCTGCAAAATGCAACTTGAGAGCTTTGGATATACAACCGATGAT
TTAAGATTCATCTGGCAGTCAGGAGATCCTGTTCAGTTGGAAAAAATTGCTTTACCTCAATTTGATATTA
AAAAGGAGGATATCGAATATGGCAACTGTACAAAATACTATAAAGGCACTGGTTACTACACTTGTGTGGA
GGTCATCTTCACCCTGAGGAGACAGGTTGGGTTCTACATGATGGGCGTATATGCACCAACCTTGCTGATT
GTGGTTCTCTCCTGGCTCTCTTTCTGGATCAACCCTGATGCTAGTGCTGCCAGAGTACCTCTGGGCATCT
TCTCCGTGCTCAGTTTGGCCTCAGAGTGCACCACCCTCGCAGCCGAGCTTCCTAAAGTGTCTTATGTGAA
GGCGCTGGATGTGTGGCTCATTGCCTGCCTGCTCTTCGGGTTTGCCTCCCTCGTGGAGTACGCTGTGGTC
CAGGTGATGCTGAACAATCCCAAAAGGGTTGAAGCCGAGAAGGCCCGAATAGCTAAGGCTGAGCAAGCAG
ATGGGAAAGGTGGAAACGCAGCTAAGAAGAATACTGTAAACGGCACGGGGACCCCTGTTCATATCAGCAC
TTTGCAGGTTGGTGAGACCAGATGCAAAAAAGTTTGTACTTCCAAGTCTGATTTGAGATCCAATGACTTC
AGCATTGTTGGAAGCTTACCAAGAGATTTTGAATTATCCAATTATGACTGCTATGGGAAACCCATCGAAG
TCAACAATGGACTTGGGAAACCACAGGCAAAGAACAAGAAGCCTCCGCCTGCCAAGCCTGTCATCCCAAC
AGCAGCCAAGCGCATCGACCTTTATGCAAGAGCATTATTTCCTTTCTGCTTCTTGTTCTTCAATGTTATA
TATTGGTCTATATATTTA


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG207969 representing BC037605
Red=Cloning site Green=Tags(s)

MKFSLAISFFILMSLLFEDACAKEKSSKKGKGKKKQYLCPSQQSPEDLARVPPNSTSNILNRLLVSYDPR
IRPNFKGIPVDVVVNIFINSFGSIQETTMDYRVNIFLRQKWNDPRLKLPSDFRGSDALTVDPTMYKCLWK
PDLFFANEKSANFHDVTQENILLFIFRDGDVLVSMRLSITLSCPLDLTLFPMDTQRCKMQLESFGYTTDD
LRFIWQSGDPVQLEKIALPQFDIKKEDIEYGNCTKYYKGTGYYTCVEVIFTLRRQVGFYMMGVYAPTLLI
VVLSWLSFWINPDASAARVPLGIFSVLSLASECTTLAAELPKVSYVKALDVWLIACLLFGFASLVEYAVV
QVMLNNPKRVEAEKARIAKAEQADGKGGNAAKKNTVNGTGTPVHISTLQVGETRCKKVCTSKSDLRSNDF
SIVGSLPRDFELSNYDCYGKPIEVNNGLGKPQAKNKKPPPAKPVIPTAAKRIDLYARALFPFCFLFFNVI
YWSIYL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC037605
ORF Size 1490 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC037605, AAH37605
RefSeq Size 3008 bp
RefSeq ORF 1490 bp
Locus ID 14658
Gene Summary This gene encodes the beta subunit of the glycine receptor, which is a pentamer composed of alpha and beta subunits. The receptor functions as a neurotransmitter-gated ion channel, which produces hyperpolarization via increased chloride conductance due to the binding of glycine to the receptor. This gene is transcribed throughout the central nervous system of neonatal and adult mice. In humans, mutations in this gene cause startle disease, also known as hereditary hyperekplexia or congenital stiff-person syndrome, a disease characterized by muscular rigidity. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2016]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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