Slc24a5 (BC094232) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	NCX5, JSX
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG208033 representing BC094232 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGACAAAATGGGGCCCAACTTGGACAAGGAGGGTCCTGCTGCTGGGGATCTTTTGGGTCTCTGCAT ACCTGCCTGTCCGTGGTGTCTCCCTGCCCCCGCGCCTCCCAAGGGCCACAGGAAACAGCACGCAATGTGC TGTCTCTCCAGCATCGGAGTTTCCCGAAGGGTTCTTCACCAAGCAGGAGAGCACGGATGGAGGTATCGTA ATCTACTTCCTCATTATCCTCTACATGTGCATGGCCATCTCCATCGTCTGCGACAAGTACTTCTTGCCCT CCCTGGAAATCATCAGTGACTCCCTTGGACTGTCGCAGGATGTTGCAGGTGCAACTTTCATGGCAGCTGG TAGCTCCGCCCCTGAACTAGTTACCGCCTTCTTGGGTGTATTTATCACAAAGGGAGATATTGGTATCAGC ACCATACTAGGATCTGCAATTTATAATCTCCTCGGCATCTGTGCTGCCTGCGGCCTCCTGTCCAACATGG TTTCAACACTCTCCTGCTGGCCCCTCTTCCGAGACTGTGCAGTGTATGCAGTTAGTGTAGGAGCAGTTTT TGGCATAATATTTGACAACCGAATTTACTGGTACGAAGGAGCAGGGCTGCTACTGATATATGGGCTCTAT GTTCTGCTGCTGTGTTTTGACACCACGATCAGCCGGCATGTCATGAAAACATGCAGTCCCTGTTGTCCCT GCCTTGCCAGAGCTATGGAGGAGAGAATTGAACAGCAGACACTGTTGGGATGGGAGGATGAGAGCCAGCT CTTCATTCGTCGCCAGTCAAGGACAGACAGTGGAATATTTCAGGAAGATTCCGGTTACTCCCAACTTTCT CTAAGCTTGCATGGTCTTAGTCAGGTTTCTGAAGATCCACCAAGTGTTTTCAGCATGCCAGAAGCAGACT TAAGAAGAATTTTTTGGGTTCTCTCTCTTCCTATTATTACATTACTTGCTCTGACAACACCAGACTGCAG GAGAAAGTTTTGGAAAAATTACTTTGTGATAACCTTTTTCATGTCTGCACTATGGATATCTGCATTCACA TACATCCTAGTTTGGATGGTCACAGTAACAGGGGAAACACTAGGGATCCCAGACACAGTGATGGGCCTTA CTTTGTTGGCAGCAGGGACAAGCATACCAGATACAGTCACAAGTGTGTTAGTTGCAAGAAAAGGTAAAGG AGACATGGCTATATCTAACATCGTGGGATCCAATGTGTTTGATATGCTATGCCTAGGTCTTCCCTGGTTT ATTAAGACTGCATTTACGAATGCATCTGCTCCTATAGAAGTGAATAGCAAGGGACTCACATATATAACAA TCTCTCTCAACATTTCAATTTTATTTCTGTTCTTAGCAGTTCATTTTAATGGCTGGAAACTAGACCGAAA GTTGGGGGTGGTCTGCCTAGTGTTATACTTAGGACTTGCTACTTTATCAGTTCTATATGAAATTGGAATT ATTGGAAATAATAGAATAAGGGGTTGTGGAGTT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG208033 representing BC094232 Red=Cloning site Green=Tags(s) MRTKWGPTWTRRVLLLGIFWVSAYLPVRGVSLPPRLPRATGNSTQCAVSPASEFPEGFFTKQESTDGGIV IYFLIILYMCMAISIVCDKYFLPSLEIISDSLGLSQDVAGATFMAAGSSAPELVTAFLGVFITKGDIGIS TILGSAIYNLLGICAACGLLSNMVSTLSCWPLFRDCAVYAVSVGAVFGIIFDNRIYWYEGAGLLLIYGLY VLLLCFDTTISRHVMKTCSPCCPCLARAMEERIEQQTLLGWEDESQLFIRRQSRTDSGIFQEDSGYSQLS LSLHGLSQVSEDPPSVFSMPEADLRRIFWVLSLPIITLLALTTPDCRRKFWKNYFVITFFMSALWISAFT YILVWMVTVTGETLGIPDTVMGLTLLAAGTSIPDTVTSVLVARKGKGDMAISNIVGSNVFDMLCLGLPWF IKTAFTNASAPIEVNSKGLTYITISLNISILFLFLAVHFNGWKLDRKLGVVCLVLYLGLATLSVLYEIGI IGNNRIRGCGV TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC094232
ORF Size	1505 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC094232, AAH94232
RefSeq Size	1882 bp
RefSeq ORF	1505 bp
Locus ID	317750
Gene Summary	Cation exchanger involved in pigmentation, possibly by participating in ion transport in melanosomes. Predominant sodium-Calcium exchanger in melanocytes. Probably transports 1 Ca(2+) and 1 K(+) to the melanosome in exchange for 4 cytoplasmic Na(+) (By similarity).[UniProtKB/Swiss-Prot Function]

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