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Tcf4 (BC014293) Mouse Tagged ORF Clone
CAT＃: MG208151
- TrueORF®
Tcf4 (tGFP-tagged) - Mouse transcription factor 4 (cDNA clone MGC:13998 IMAGE:4014231)
ORF Plasmid: DDK
"BC014293" in other vectors (3)
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CNY 3,140.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | ME2, ITF-2, SEF-2, MITF-2A, MITF-2B, TFE, E2-2, E2.2, ITF2, SEF2, ASP-I2, ITF-2b, SEF2-1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG208151 representing BC014293
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTACTGCGCATACACCATCCCGGGCATGGGCGGCAACTCTTTGATGTACTACTATAATGGGAAAGCGG TCTACGCTCCTTCAGCCAGCACTGCCGACTACAACAGGGATTCGCCAGGCTATCCTTCCTCCAAGCCAGC AGCCAGCACTTTCCCTAGCTCCTTCTTCATGCAAGATGGCCATCACAGCAGCGACCCTTGGAGCTCCTCC AGCGGGATGAATCAGCCCGGCTACGGAGGGATGCTGGGCAATTCTTCTCATATCCCACAGTCCAGCAGCT ACTGTAGCCTGCATCCACATGAACGTTTGAGCTATCCATCCCACTCCTCGGCAGACATCAACTCCAGTCT TCCTCCGATGTCCACGTTCCATCGTAGTGGCACAAACCATTACAGCACCTCTTCCTGCACACCCCCTGCC AACGGAACAGACAGTATAATGGCAAACAGAGGAACTGGGGCAGCAGGCAGCTCCCAGACTGGAGACGCTC TGGGGAAAGCCCTAGCTTCGATCTATTCTCCTGACCACACGAACAACAGCTTTTCCTCCAATCCTTCAAC TCCTGTGGGCTCCCCTCCTTCACTCTCAGCAGGCACAGCTGTTTGGTCTAGAAATGGAGGACAGGCCTCG TCATCTCCCAATTATGAAGGACCCTTGCACTCACTGCAAAGCCGAATCGAAGACCGTTTGGAAAGACTGG ACGATGCGATTCATGTTCTCCGGAACCACGCAGTGGGCCCGTCCACAGCTGTGCCTGGTGGGCATGGGGA CATGCATGGGATCATGGGACCCTCCCACAACGGAGCGATGGGTAGCCTGGGCTCAGGGTATGGAACTAGT CTTCTCTCAGCCAACAGACACTCGCTCATGGTTGGGGCCCACCGTGAAGATGGCGTGGCTCTGAGAGGCA GCCATTCTCTCCTGCCAAACCAGGTTCCGGTCCCACAACTTCCGGTCCAGTCTGCAACTTCCCCTGACTT GAACCCACCCCAAGACCCTTACAGAGGGATGCCACCAGGTCTCCAGGGCCAGAGCGTGTCTTCTGGTAGC TCTGAGATCAAATCCGACGACGAGGGCGATGAGAACCTGCAAGACACAAAATCTTCTGAGGACAAGAAAT TAGATGACGACAAGAAGGATATCAAATCAATTACTAGCAATAACGATGATGAGGACCTGACCCCAGAGCA GAAGGCTGAGCGCGAGAAGGAACGGAGGATGGCCAATAATGCCCGTGAGCGCCTGAGGGTCCGAGATATC AACGAGGCTTTCAAGGAGCTTGGCCGTATGGTGCAGCTCCACCTGAAGAGCGACAAGCCCCAGACCAAGC TCCTGATTCTCCACCAGGCCGTGGCTGTCATCCTCAGCCTGGAGCAGCAAGTTCGAGAAAGGAATCTGAA CCCGAAAGCTGCCTGTCTGAAAAGAAGGGAGGAAGAGAAGGTGTCCTCAGAGCCTCCCCCACTCTCCTTG GCTGGCCCACACCCTGGGATGGGAGACGCAGCGAATCACATGGGACAGATG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG208151 representing BC014293
Red=Cloning site Green=Tags(s) MYCAYTIPGMGGNSLMYYYNGKAVYAPSASTADYNRDSPGYPSSKPAASTFPSSFFMQDGHHSSDPWSSS SGMNQPGYGGMLGNSSHIPQSSSYCSLHPHERLSYPSHSSADINSSLPPMSTFHRSGTNHYSTSSCTPPA NGTDSIMANRGTGAAGSSQTGDALGKALASIYSPDHTNNSFSSNPSTPVGSPPSLSAGTAVWSRNGGQAS SSPNYEGPLHSLQSRIEDRLERLDDAIHVLRNHAVGPSTAVPGGHGDMHGIMGPSHNGAMGSLGSGYGTS LLSANRHSLMVGAHREDGVALRGSHSLLPNQVPVPQLPVQSATSPDLNPPQDPYRGMPPGLQGQSVSSGS SEIKSDDEGDENLQDTKSSEDKKLDDDKKDIKSITSNNDDEDLTPEQKAEREKERRMANNARERLRVRDI NEAFKELGRMVQLHLKSDKPQTKLLILHQAVAVILSLEQQVRERNLNPKAACLKRREEEKVSSEPPPLSL AGPHPGMGDAANHMGQM TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC014293 |
| ORF Size | 1523 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC014293, AAH14293 |
| RefSeq Size | 1765 bp |
| RefSeq ORF | 1523 bp |
| Locus ID | 21413 |
| Gene Summary | Transcription factor that binds to the immunoglobulin enhancer Mu-E5/KE5-motif. Involved in the initiation of neuronal differentiation. Activates transcription by binding to the E box (5'-CANNTG-3'). Isoform 2 inhibits MYOD1 activation of the cardiac alpha-actin promoter. Binds to the E-box present in the somatostatin receptor 2 initiator element (SSTR2-INR) to activate transcription. May have a regulatory function in developmental processes as well as during neuronal plasticity.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR208151 | Tcf4 (Myc-DDK-tagged) - Mouse transcription factor 4 (cDNA clone MGC:13998 IMAGE:4014231) |
CNY 3,776.00 |
|
| MR208151L3 | Lenti ORF clone of Tcf4 (Myc-DDK-tagged) - Mouse transcription factor 4 (cDNA clone MGC:13998 IMAGE:4014231) |
CNY 4,750.00 |
|
| MR208151L4 | Lenti ORF clone of Tcf4 (mGFP-tagged) - Mouse transcription factor 4 (cDNA clone MGC:13998 IMAGE:4014231) |
CNY 4,750.00 |
