Slc2a4 (NM_009204) Mouse Tagged ORF Clone

CAT#: MG208202

  • TrueORF®

Slc2a4 (tGFP-tagged) - Mouse solute carrier family 2 (facilitated glucose transporter), member 4 (Slc2a4)

ORF Plasmid: DDK tGFP



  "NM_009204" in other vectors (3)

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Mouse Protein / Lysate Request

CNY 5,392.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


Goat Anti-SLC2A4 / GLUT4 Antibody
    • 100 ug

CNY 5,371.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms Glut-4; Glut4; GT2; twgy
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG208202 representing NM_009204
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCGTCGGGTTTCCAGCAGATCGGCTCTGACGATGGGGAACCCCCTCGGCAGCGAGTGACTGGAACAC
TGGTCCTAGCTGTATTCTCAGCTGTGCTTGGCTCCCTTCAGTTTGGCTATAACATTGGGGTTATCAATGC
CCCACAGAAGGTGATTGAACAGAGCTACAATGCAACGTGGCTGGGTAGGCAAGGTCCTGGGGGACCGGAT
TCCATCCCACAAGGCACCCTCACTACGCTCTGGGCTCTCTCCGTGGCCATCTTCTCTGTGGGTGGCATGA
TCTCTTCCTTTCTCATTGGCATCATTTCTCAATGGTTGGGAAGGAAAAGGGCTATGCTGGCCAACAATGT
CTTGGCCGTGTTGGGGGGCGCCCTCATGGGCCTAGCCAATGCCGTGGCCTCCTATGAGATACTCATTCTT
GGACGGTTCCTCATTGGCGCCTACTCAGGGCTAACATCAGGGCTGGTGCCCATGTATGTGGGAGAAATCG
CCCCCACTCATCTTCGGGGTGCCTTGGGAACACTCAACCAACTGGCCATCGTCATTGGCATTCTGGTTGC
CCAGGTGCTGGGCTTGGAGTCTATGCTGGGCACAGCTACCCTGTGGCCACTGCTTCTGGCTCTCACAGTA
CTCCCTGCTCTCCTGCAGCTGATTCTGCTGCCCTTCTGTCCTGAGAGCCCCAGATACCTCTACATCATCC
GGAACCTGGAGGGGCCTGCCCGAAAGAGTCTAAAGCGCCTGACCGGCTGGGCTGATGTGTCTGACGCACT
AGCTGAGCTGAAGGATGAGAAACGGAAGTTGGAGAGAGAGCGTCCAATGTCCTTGCTCCAGCTCCTGGGC
AGCCGCACCCACCGGCAGCCTCTTATCATCGCAGTGGTGCTGCAGCTGAGCCAACAGCTCTCAGGCATCA
ATGCTGTTTTCTACTATTCAACCAGCATCTTCGAGTCGGCTGGGGTGGGACAGCCAGCCTACGCCACCAT
AGGAGCTGGTGTGGTCAATACGGTCTTCACGTTGGTCTCGGTGCTCTTAGTAGAACGAGCTGGACGACGG
ACACTCCATCTGTTGGGCCTGGCCGGCATGTGTGGCTGTGCCATCTTGATGACCGTGGCTCTGCTGCTGC
TGGAACGGGTTCCAGCCATGAGCTATGTCTCCATCGTGGCCATATTTGGCTTTGTGGCCTTCTTTGAGAT
TGGCCCTGGCCCCATTCCCTGGTTCATTGTGGCAGAGCTCTTCAGCCAGGGCCCCCGCCCAGCCGCCATG
GCTGTCGCTGGTTTCTCCAACTGGACCTGTAACTTCATTGTCGGCATGGGTTTCCAGTATGTTGCGGATG
CTATGGGTCCTTACGTCTTCCTTCTATTTGCCGTCCTCCTGCTTGGCTTCTTCATCTTCACCTTCCTAAA
AGTGCCTGAAACCAGAGGCCGGACGTTTGACCAGATCTCAGCTGCCTTCCGACGGACACCTTCCCTTTTA
GAGCAGGAGGTGAAACCCAGTACAGAACTTGAATACTTAGGGCCAGATGAGAATGAC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG208202 representing NM_009204
Red=Cloning site Green=Tags(s)

MPSGFQQIGSDDGEPPRQRVTGTLVLAVFSAVLGSLQFGYNIGVINAPQKVIEQSYNATWLGRQGPGGPD
SIPQGTLTTLWALSVAIFSVGGMISSFLIGIISQWLGRKRAMLANNVLAVLGGALMGLANAVASYEILIL
GRFLIGAYSGLTSGLVPMYVGEIAPTHLRGALGTLNQLAIVIGILVAQVLGLESMLGTATLWPLLLALTV
LPALLQLILLPFCPESPRYLYIIRNLEGPARKSLKRLTGWADVSDALAELKDEKRKLERERPMSLLQLLG
SRTHRQPLIIAVVLQLSQQLSGINAVFYYSTSIFESAGVGQPAYATIGAGVVNTVFTLVSVLLVERAGRR
TLHLLGLAGMCGCAILMTVALLLLERVPAMSYVSIVAIFGFVAFFEIGPGPIPWFIVAELFSQGPRPAAM
AVAGFSNWTCNFIVGMGFQYVADAMGPYVFLLFAVLLLGFFIFTFLKVPETRGRTFDQISAAFRRTPSLL
EQEVKPSTELEYLGPDEND

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_009204
ORF Size 1527 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_009204.2, NP_033230.2
RefSeq Size 2822 bp
RefSeq ORF 1530 bp
Locus ID 20528
UniProt ID P14142
Gene Summary Insulin-regulated facilitative glucose transporter, which plays a key role in removal of glucose from circulation (PubMed:26240143, PubMed:26629404). Response to insulin is regulated by its intracellular localization: in the absence of insulin, it is efficiently retained intracellularly within storage compartments in muscle and fat cells (PubMed:26240143, PubMed:26629404). Upon insulin stimulation, translocates from these compartments to the cell surface where it transports glucose from the extracellular milieu into the cell (PubMed:26240143, PubMed:26629404).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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