Arcn1 (BC017124) Mouse Tagged ORF Clone

CAT#: MG208214

  • TrueORF®

Arcn1 (tGFP-tagged) - Mouse archain 1 (cDNA clone MGC:27546 IMAGE:4480874)

ORF Plasmid: DDK tGFP



  "BC017124" in other vectors (3)

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Mouse Protein / Lysate Request

CNY 5,610.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms MGC27546
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG208214 representing BC017124
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGCTGTTGGCAGCAGCAGTCTGCACGAAAGCGGGAAAGGCTATTGTTTCTCGACAGTTCGTGGAGA
TGACCCGAACTCGGATTGAAGGCTTATTAGCAGCTTTCCCAAAACTCATGAACACTGGAAAACAACATAC
TTTTGTTGAAACAGAGAGTGTAAGATATGTGTACCAACCTATGGAGAAACTGTACATGGTATTGATCACT
ACAAAGAACAGCAATATCTTAGAAGATTTGGAGACTTTAAGGCTCTTCTCAAGGGTGATCCCTGAATATT
GCCGAGCCTTAGAGGAAAATGAAATATCTGAGCACTGTTTTGATTTGATTTTTGCTTTTGATGAAATTGT
TGCCCTGGGATACCGGGAGAATGTTAACCTGGCACAGATCAGAACCTTTACAGAAATGGACTCTCACGAG
GAGAAAGTATTCCGAGCAGTCAGAGAGACTCAAGAACGTGAGGCTAAGGCTGAGATGCGGCGTAAAGCAA
AGGAATTACAACAGGCCCGAAGAGATGCAGAGAGACAGGGGAAAAAAGCACCAGGATTTGGCGGATTTGG
TAGTTCTGCAGTGTCTGGAGGCAGCACAGCAGCCATGATCACAGAGACTATCATTGAAACTGATAAACCA
AAAGTGGCGCCTGCACCAGCCAGACCTTCAGGCCCCAGCAAGGCTTTGAAACTTGGAGCTAAAGGAAAGG
AAGTAGATAACTTTGTGGACAAATTGAAATCTGAAGGTGAAACTATTATGTCTTCTAATATGGGAAAACG
TACCTCAGAAGCAACCAAAGTGCATGCTCCACCTATTAATATGGAAAGTGTGCACATGAAGATTGAAGAG
AAGATCACACTAACCTGTGGGAGAGATGGAGGATTACAGAATATGGAGTTGCATGGCATGATCATGCTTA
GGATCTCAGATGACAAATTTGGCCGGATTCGTCTTCATGTAGAAAATGAAGATAAGAAAGGGGTGCAGCT
ACAGACGCATCCAAATGTGGATAAAAAACTTTTTACTGCAGAGTCTCTCATTGGCTTGAAAAACCCAGAG
AAGTCATTTCCAGTCAACAGTGATGTAGGGGTACTAAAGTGGAGACTACAAACAACAGAGGAATCTTTTA
TTCCGTTGACAATTAATTGCTGGCCTTCGGAAAGTGGAAATGGCTGTGATGTCAACATAGAGTACGAACT
ACAGGAAGATAATTTAGAGCTCAATGATGTGGTCATCACTATCCCACTCCCATCGGGTGTTGGCGCGCCT
GTGATTGGTGAGATCGATGGGGAATATCGACATGACAGTCGGAGAAATACGTTGGAGTGGTGCCTGCCAG
TGATTGATGCCAAAAATAAGAGTGGCAGCCTGGAGTTCAGCATTCCTGGGCAGCCTAATGACTTCTTCCC
TGTTCAAGTTTCTTTCATCTCCAAAAAGAATTACTGCAACATACAGGTTACCAAAGTGACCCAGGTGGAT
GGAAACAGCCCCGTCAGGTTTTCCACGGAGACCACTTTCTTAGTGGACAAATACGAAATCCTG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG208214 representing BC017124
Red=Cloning site Green=Tags(s)

MVLLAAAVCTKAGKAIVSRQFVEMTRTRIEGLLAAFPKLMNTGKQHTFVETESVRYVYQPMEKLYMVLIT
TKNSNILEDLETLRLFSRVIPEYCRALEENEISEHCFDLIFAFDEIVALGYRENVNLAQIRTFTEMDSHE
EKVFRAVRETQEREAKAEMRRKAKELQQARRDAERQGKKAPGFGGFGSSAVSGGSTAAMITETIIETDKP
KVAPAPARPSGPSKALKLGAKGKEVDNFVDKLKSEGETIMSSNMGKRTSEATKVHAPPINMESVHMKIEE
KITLTCGRDGGLQNMELHGMIMLRISDDKFGRIRLHVENEDKKGVQLQTHPNVDKKLFTAESLIGLKNPE
KSFPVNSDVGVLKWRLQTTEESFIPLTINCWPSESGNGCDVNIEYELQEDNLELNDVVITIPLPSGVGAP
VIGEIDGEYRHDSRRNTLEWCLPVIDAKNKSGSLEFSIPGQPNDFFPVQVSFISKKNYCNIQVTKVTQVD
GNSPVRFSTETTFLVDKYEIL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC017124
ORF Size 1533 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC017124.1
RefSeq Size 3464 bp
RefSeq ORF 1535 bp
Locus ID 213827
Gene Summary The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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