Rod1 (BC006638) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	MGC11742
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG208328 representing BC006638 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACAGCTCGACTTCTGCAGCTAATGGGAATGACAACAAGAAATTTAAAGGAGATAGACCTCCCTGTT CGCCTTCCCGTGTTCTCCATCTTCGAAAAATTCCATGTGATGTCACCGAAGCAGAGGTCATATCATTAGG TCTACCATTTGGCAAAGTAACTAATCTTTTGATGTTGAAAGGAAAAAGCCAGGCCTTTTTAGAAATGGCT TCTGAAGAAGCTGCTGTTACTATGATAAATTATTACACTCCTGTTACTCCTCATCTTCGAAGTCAGCCTG TTTATATCCAGTATTCCAATCACCGAGAACTTAAGACTGACAATCTGCCTAATCAGGCTAGAGCCCAAGC TGCACTGCAGGCTGTCAGTGCAGTCCAGTCAGGAAACTTGTCCCTTCCTGGAGCTACTGCAAATGAAGGC ACATTGCTACCTGGGCAGAGCCCTGTGCTCCGGATAATTATTGAAAACCTATTTTACCCTGTCACTCTGG AAGTTCTCCATCAGATATTTTCTAAATTTGGCACGGTGTTGAAGATTATCACCTTTACAAAGAATAATCA GTTTCAAGCCTTGCTTCAGTATGCCGACCCAGTGAATGCGCAGTATGCCAAAATGGCTCTGGATGGCCAG AATATCTACAATGCGTGCTGCACTCTCCGTATTGACTTCTCCAAGCTCACCAGCCTTAATGTGAAATACA ATAATGACAAAAGCAGAGACTTCACTCGCTTAGACCTTCCTACTGGTGACGGCCAGCCATCTCTTGAACC TCCTATGGCTGCTGCTTTTGGTGCACCGGGTATAATGTCCTCTCCATATGCAGGGGCTGCTGGCTTTGCC CCAGCCATCGCATTTCCTCAAGCAGCAGGTCTGTCTGTCCCAGCTGTTCCCGGAGCCCTTGGTCCTCTCA CACTTACCTCCTCTGCTGTCAGTGGAAGGATGGCCATTCCTGGGGCAAGTGGTATGCCTGGGAATTCTGT TCTGCTGGTCACCAACCTCAATCCTGACTTTATCACACCACATGGGCTTTTTATCCTATTTGGAGTGTAT GGTGATGTACATCGAGTGAAGATTATGTTTAACAAGAAAGAAAACGCCTTGGTGCAGATGGCGGATGCGA GTCAAGCCCAGCTAGCAATGAACCACCTAAGTGGTCAGAGGCTCTATGGAAAAGTACTTCGGGCTACACT GTCCAAACATCAAGCCGTCCAGCTCCCTAGAGAGGGACAGGAAGACCAAGGTCTGACCAAGGACTTCAGC AATAGTCCCCTGCATCGCTTTAAAAAGCCCGGCTCAAAGAATTTCCAAAATATCTTCCCTCCATCAGCCA CACTTCATCTTTCCAACATTCCTCCTTCTGTCACAATGGATGACCTGAAGAACCTGTTCACAGAAGCTGG ATGCTCAGTGAAGGCTTTTAAGTTCTTCCAGAAAGACCGTAAGATGGCTCTCATTCAGTTGGGATCTGTG GAAGAAGCAATCCAGGCTCTTATTGAGCTTCACAATCATGACCTTGGAGAGAATCACCACCTCAGAGTTT CCTTCTCAAAATCCACCATC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG208328 representing BC006638 Red=Cloning site Green=Tags(s) MNSSTSAANGNDNKKFKGDRPPCSPSRVLHLRKIPCDVTEAEVISLGLPFGKVTNLLMLKGKSQAFLEMA SEEAAVTMINYYTPVTPHLRSQPVYIQYSNHRELKTDNLPNQARAQAALQAVSAVQSGNLSLPGATANEG TLLPGQSPVLRIIIENLFYPVTLEVLHQIFSKFGTVLKIITFTKNNQFQALLQYADPVNAQYAKMALDGQ NIYNACCTLRIDFSKLTSLNVKYNNDKSRDFTRLDLPTGDGQPSLEPPMAAAFGAPGIMSSPYAGAAGFA PAIAFPQAAGLSVPAVPGALGPLTLTSSAVSGRMAIPGASGMPGNSVLLVTNLNPDFITPHGLFILFGVY GDVHRVKIMFNKKENALVQMADASQAQLAMNHLSGQRLYGKVLRATLSKHQAVQLPREGQEDQGLTKDFS NSPLHRFKKPGSKNFQNIFPPSATLHLSNIPPSVTMDDLKNLFTEAGCSVKAFKFFQKDRKMALIQLGSV EEAIQALIELHNHDLGENHHLRVSFSKSTI TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC006638
ORF Size	1560 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC006638, AAH06638
RefSeq Size	3417 bp
RefSeq ORF	1562 bp
Locus ID	230257
Gene Summary	RNA-binding protein that mediates pre-mRNA alternative splicing regulation. Plays a role in the regulation of cell proliferation, differentiation and migration. Positive regulator of EPO-dependent erythropoiesis. Participates in cell differentiation regulation by repressing tissue-specific exons. Promotes Fas exon 6 skipping. Binds RNA, preferentially to both poly(G) and poly(U) (By similarity).[UniProtKB/Swiss-Prot Function]

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