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Arfgap2 (NM_023854) Mouse Tagged ORF Clone
CAT＃: MG208335
- TrueORF®
Arfgap2 (tGFP-tagged) - Mouse zinc finger protein 289 (Zfp289)
ORF Plasmid: DDK
"NM_023854" in other vectors (4)
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CNY 5,040.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 2310032E02Rik; Zfp28; Zfp289 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG208335 representing NM_023854
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGAGCCCGAGCAAGACCGAAATCCAGACTATTTTTAAGAGACTTCGCGCAATTCCCACCAACA AGGCCTGTTTCGATTGTGGCGCCAAGAGTCCTAGTTGGGCCAGCATCACTTACGGTGTATTTTTGTGTAT TGACTGCTCCGGAGTGCATCGCTCCTTGGGTGTCCATCTCAGCTTTATCAGGTCCACAGAGTTGGATTCG AACTGGAGCTGGTTGCAGCTGAGGTGTATGCAGGTCGGCGGGAATGCCAATGCGACTGCTTTTTTCCGCC AACACGGATGTATGGCCAATGATGCTAACACCAAATATACGAGCCGAGCTGCCCAGATGTACCGAGAGAA GATCCGGCAGTTAGGGAGTGCAGCTCTGACTAGGCATGGTACTGATCTTTGGATAGACAGTATGAACAGT GCTCCAAGTCACTCTCCAGAGAAGAAAGACTCTGATTTCTTCACAGAACATACTCAGGCCCCTGCTTGGG ATACAGCAGCCACTGATCCTTCAGGGACTCAGCAACCAGCCCTACCTTCAGAGAGCAGTAGCCTGGCACA ACCTGAACAAGGCCCTAACACTGATCTGCTCGGAACCTCACCCCAAGCCTCTCTGGAACTGAAAAGCTCC ATTATTGGCAAGAAGAAGCCAGCAGCCGCTAAGAAAGGGCTGGGTGCCAAGAAAGGCCTGGGAGCTCAGA AGGTGAGCAACCAGAGCTTTACTGAGATTGAGCGGCAGGCCCAGGTGGCAGAGAAGCTCCGAGAGCAGCA GGCAGCTGATGCCAAGAAGCAGGCAGAAGAGTCCATGGTTGCCTCCATGCGTCTGGCCTACCAGGAGCTC CAGATTGATCGCAAGAAGGAGGAGAAAAAGCTCCAGAATCTTGAAGGAAAGAAGCGAGAGCAGGCTGAAA GGCTGGGCATGGGCTTGGTGTCCCGAAGCTCCATCTCCCATTCTGTGCTGTCTGAGATGCAGATGATTGA GCAGGAAACCCCGCTGAGTGCGAAATCTTCCCGTTCACAGCTTGACTTGTTTGATGATGTTGGAACATTT GCATCTGGACCCCCAAAGTACAAGGACAACCCCTTTTCCTTGGGAGAAACTTTTGGTTCCCGCTGGGATT CAGATGCAGCCTGGGGTATGGACAGGGTAGAAGAGAAGGAACCTGAAGTTACCATCTCCAGCATCCGGCC TATTTCAGAAAGAACTGCGAGTCGGAGGGAAGTGGAGACTCGGAGCTCAGGCCTTGAGTCCAGTGAAGCA CGGCAGAAGTTTGCAGGAGCCAAAGCCATCTCGTCCGACATGTTCTTTGGTCGTGAGGTGGATTCTGAGT ATGAAGCTAGGTCTCGGTTACAGCAGCTTTCGGGCAGCAGTGCCATCAGCTCTTCAGACCTTTTTGGAAA CATGGATGGAGCTCATGGAGGAGGAACTGTATCTCTGGGGAATGTACTTCCTACAGCTGACATTGCCCAG TTTAAGCAGGGTGTCAAGTCTGTGGCTGGCAAGATGGCTGTGCTGGCCAATGGTGTGATGAATTCCTTAC AGGATCGCTACGGTTCCTAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG208335 representing NM_023854
Red=Cloning site Green=Tags(s) MAASPSKTEIQTIFKRLRAIPTNKACFDCGAKSPSWASITYGVFLCIDCSGVHRSLGVHLSFIRSTELDS NWSWLQLRCMQVGGNANATAFFRQHGCMANDANTKYTSRAAQMYREKIRQLGSAALTRHGTDLWIDSMNS APSHSPEKKDSDFFTEHTQAPAWDTAATDPSGTQQPALPSESSSLAQPEQGPNTDLLGTSPQASLELKSS IIGKKKPAAAKKGLGAKKGLGAQKVSNQSFTEIERQAQVAEKLREQQAADAKKQAEESMVASMRLAYQEL QIDRKKEEKKLQNLEGKKREQAERLGMGLVSRSSISHSVLSEMQMIEQETPLSAKSSRSQLDLFDDVGTF ASGPPKYKDNPFSLGETFGSRWDSDAAWGMDRVEEKEPEVTISSIRPISERTASRREVETRSSGLESSEA RQKFAGAKAISSDMFFGREVDSEYEARSRLQQLSGSSAISSSDLFGNMDGAHGGGTVSLGNVLPTADIAQ FKQGVKSVAGKMAVLANGVMNSLQDRYGSY TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_023854 |
| ORF Size | 1560 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_023854.2, NP_076343.2 |
| RefSeq Size | 2752 bp |
| RefSeq ORF | 1563 bp |
| Locus ID | 77038 |
| UniProt ID | Q99K28 |
| Gene Summary | This gene encodes a zinc-finger-containing GTPase-activating protein for ADP ribosylation factor 1 (ARF1), a small GTPase that plays a role in coatomer-mediated vesicular trafficking. This gene product stimulates the hydrolysis of ARF1-bound GTP, which may lead to dissociation of coatomer from Golgi-derived membranes to allow fusion with target membranes. It may regulate the retrograde transport from the Golgi complex to the endoplasmic reticulum. Expression of this gene has been shown to be controlled by inhibitor of DNA binding 1 (Id1). Alternatively spliced transcript variants encoding different isoforms have been found for this gene. A pseudogene of this gene was identified on chromosome 6. [provided by RefSeq, Oct 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC217400 | Arfgap2 (untagged) - Mouse ADP-ribosylation factor GTPase activating protein 2 (Arfgap2), transcript variant 2, (10ug) |
CNY 6,750.00 |
|
| MR208335 | Arfgap2 (Myc-DDK-tagged) - Mouse ADP-ribosylation factor GTPase activating protein 2 (Arfgap2), transcript variant 2 |
CNY 3,880.00 |
|
| MR208335L3 | Lenti ORF clone of Arfgap2 (Myc-DDK-tagged) - Mouse ADP-ribosylation factor GTPase activating protein 2 (Arfgap2), transcript variant 2 |
CNY 6,460.00 |
|
| MR208335L4 | Lenti ORF clone of Arfgap2 (mGFP-tagged) - Mouse ADP-ribosylation factor GTPase activating protein 2 (Arfgap2), transcript variant 2 |
CNY 6,460.00 |
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