Pak2 (BC086650) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	PAK-2, mKIAA4182
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG208417 representing BC086650 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGATAACGGAGAGCTAGAAGACAAGCCCCCAGCACCTCCAGTTCGGATGAGCAGTACCATTTTTA GCACCGGAGGAAAAGATCCTTTATCAGCCAATCACAGTTTGAAACCTTTGCCTTCTGTTCCAGAGGAAAA AAAACCCAGGAACAAAATCATCTCCATATTCTCTGGCACAGAAAAAGGAAGTAAAAAGAAAGAAAAAGAA CGGCCAGAGATTTCTCCCCCATCTGATTTTGAGCACACCATCCATGTTGGCTTTGATGCTGTTACGGGAG AGTTCACTGGCATGCCAGAACAGTGGGCGCGGCTGTTGCAGACCTCCAACATTACCAAACTCGAGCAGAA GAAGAACCCTCAGGCAGTGCTGGATGTCTTGAAGTTCTACGACTCCAACACTGTGAAACAGAAGTACCTG AGTTTCACTCCTCCTGAGAAAGATGGCTTCCCTTCTGGAACACCAGCACTGAACACCAAGGGGTCAGAGA CATCAGCTGTAGTGACAGAGGAAGATGATGATGATGAAGACGCTGCTCCTCCCGTCATTGCCCCTCGGCC AGATCATACAAAATCAATTTACACACGGTCTGTCATCGACCCCATTCCTGCTCCAGTTGGTGATTCTAAT GTTGACAGTGGTGCCAAGTCTTCAGACAAACAGAAAAAGAAAGCCAAGATGACCGATGAAGAGATTATGG AGAAATTAAGAACTATTGTGAGCATAGGGGACCCAAAGAAAAAATACACAAGATATGAAAAAATTGGGCA AGGGGCTTCTGGAACAGTTTTTACTGCCACTGATGTGGCCCTGGGGCAAGAGGTTGCTATCAAGCAGATT AATTTACAGAAACAACCAAAGAAGGAATTGATCATTAATGAAATTCTGGTGATGAAAGAGTTAAAGAATC CCAACATAGTTAACTTCTTGGACAGTTACCTGGTAGGAGATGAGTTGTTTGTGGTAATGGAGTACCTCGC TGGTGGGTCCCTCACTGATGTTGTAACAGAAACCTGCATGGACGAAGCGCAGATTGCCGCCGTGTGCAGA GAGTGTTTACAGGCGTTGGAGTTTTTACATGCTAATCAAGTGATCCACAGAGACATCAAAAGTGACAATG TGCTTTTGGGAATGGAAGGCTCAGTTAAACTTACTGACTTCGGCTTCTGTGCCCAGATCACTCCTGAACA GAGCAAACGCAGTACTATGGTTGGAACACCGTACTGGATGGCACCAGAGGTGGTGACACGGAAAGCCTAT GGTCCCAAAGTTGACATATGGTCTCTGGGCATCATGGCTATCGAGATGGTTGAAGGAGAGCCTCCATACC TCAACGAAAATCCTCTGCGGGCATTATACCTGATAGCTACAAATGGAACTCCTGAACTTCAGAATCCAGA AAAACTTTCCCCAATATTTCGGGATTTCTTAAATCGGTGTTTGGAAATGGATGTGGAGAAAAGGGGTTCG GCCAAGGAACTGTTACAGCATCCTTTCCTGAAACTGGCCAAACCATTGTCTAGCTTGACGCCACTGATCC TGGCAGCTAAAGAAGCAATGAAGAGTAACCGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG208417 representing BC086650 Red=Cloning site Green=Tags(s) MSDNGELEDKPPAPPVRMSSTIFSTGGKDPLSANHSLKPLPSVPEEKKPRNKIISIFSGTEKGSKKKEKE RPEISPPSDFEHTIHVGFDAVTGEFTGMPEQWARLLQTSNITKLEQKKNPQAVLDVLKFYDSNTVKQKYL SFTPPEKDGFPSGTPALNTKGSETSAVVTEEDDDDEDAAPPVIAPRPDHTKSIYTRSVIDPIPAPVGDSN VDSGAKSSDKQKKKAKMTDEEIMEKLRTIVSIGDPKKKYTRYEKIGQGASGTVFTATDVALGQEVAIKQI NLQKQPKKELIINEILVMKELKNPNIVNFLDSYLVGDELFVVMEYLAGGSLTDVVTETCMDEAQIAAVCR ECLQALEFLHANQVIHRDIKSDNVLLGMEGSVKLTDFGFCAQITPEQSKRSTMVGTPYWMAPEVVTRKAY GPKVDIWSLGIMAIEMVEGEPPYLNENPLRALYLIATNGTPELQNPEKLSPIFRDFLNRCLEMDVEKRGS AKELLQHPFLKLAKPLSSLTPLILAAKEAMKSNR TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC086650
ORF Size	1574 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC086650, AAH86650
RefSeq Size	4135 bp
RefSeq ORF	1574 bp
Locus ID	224105
Gene Summary	Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell motility, cell cycle progression, apoptosis or proliferation. Acts as downstream effector of the small GTPases CDC42 and RAC1. Activation by the binding of active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Full-length PAK2 stimulates cell survival and cell growth. Phosphorylates MAPK4 and MAPK6 and activates the downstream target MAPKAPK5, a regulator of F-actin polymerization and cell migration. Phosphorylates JUN and plays an important role in EGF-induced cell proliferation. Phosphorylates many other substrates including histone H4 to promote assembly of H3.3 and H4 into nucleosomes, BAD, ribosomal protein S6, or MBP. Additionally, associates with ARHGEF7 and GIT1 to perform kinase-independent functions such as spindle orientation control during mitosis. On the other hand, apoptotic stimuli such as DNA damage lead to caspase-mediated cleavage of PAK2, generating PAK-2p34, an active p34 fragment that translocates to the nucleus and promotes cellular apoptosis involving the JNK signaling pathway. Caspase-activated PAK2 phosphorylates MKNK1 and reduces cellular translation (By similarity).[UniProtKB/Swiss-Prot Function]

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