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Ncf2 (NM_010877) Mouse Tagged ORF Clone
CAT＃: MG208432
- TrueORF®
Ncf2 (tGFP-tagged) - Mouse neutrophil cytosolic factor 2 (Ncf2)
ORF Plasmid: DDK
"NM_010877" in other vectors (4)
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CNY 5,040.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Ncf-2; NOXA2; p67phox |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG208432 representing NM_010877
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCCTGGCTGAGGCCATCAGACTCTGGAATGAAGGGGTGCTCGCAGCCGACAAGAAGGACTGGAAGG GGGCCCTGGAGGCCTTCAGCGAGGTGCAGGACCCCCACTCGAGGATTTGCTTCAACATAGGCTGCGTGAA CACCATCCTGGAAAACTTGCAGGCAGCCGAGCAGGCCTTCACCAAAAGCATCAACAGAGACAAGCACTCT GCAGTGGCCTACTTCCAGAGAGGAATGCTCTACTACAGAATGGAGAAGTACGACCTTGCTATCAAAGACC TTAAAGAGGCCTTGACGCAGCTTCGTGGGAACCAGCTGATAGACTACAAGATCCTGGGGCTGCAGTTCAA GCTGTTTGCCTGTGAGGTATTGTACAATATTGCTCTCATGCATGCCAAGAAAGAGGAATGGAAGAAAGCA GAAGAGCAGTTGGCATTGGCAACCAACATGAAGTCCGAGCCCAGGCATTCCAAGATCGACAAGGCCATGG AGAGCATCTGGAAGCAGAAGCTGTTCGAGCCCGTGGTGATCCCTGTGGGTCGGCTGTTCCGTCCAAATGA GAGGCAGGTGGCTCAGCTGGCCAAAAAGGACTATCTGGGCAAGGCTACGGTTGTAGCATCTGTGGTTCAC CAAGACAACTTTTCTGGCTTCGCCCCTCTGCAGCCGCAGTCAGCAGAGCCTCCTCCCAGACCCAAAACCC CAGAAATCTTCAGGGCTCTGGAAGGTGAGGCACACCGCGTATTGTTTGGCTTTGTGCCGGAGACGCCAGA AGAGCTACAGGTCATGCCTGGGAACATCGTCTTTGTCTTGAAGAAGGGCAGTGATAACTGGGCCACAGTC ATGTTCAATGGACAGAAGGGGCTTGTCCCCTGCAACTACCTGGAGCCAGTTGAGCTTCGGATTCACCCTC AGTCGCAGCCCCAGGAAGATACCTCTCCAGAATCTGATATTCCACCACCTCCTAATTCTAGTCCCCCAGG AAGACTCCAGTTGTCACCAGGTCACAAGCAAAAAGAGCCCAAGGAACTGAAGCTCAGCGTGCCTATGCCT TACATGCTCAAGGTGCATTACAAATACACAGTGGTCATGGAGACGCGGCTTGGCCTCCCCTACAGCCAGC TTCGGAACATGGTGTCTAAGAAGCTGGCGCTCTCGCCAGAACACACTAAACTGAGCTACCGGCGTCGGGA CAGCCACGAGCTTCTGCTCCTGTCCGAAGAAAGCATGAAGGATGCCTGGGGCCAAGTGAAAAACTACTGC CTGACTCTGTGGTGTGAGCATACGGTGGGTGACCAAGGTCTTATTGATGAACCCATACAAAGGGAAAACT CAGACGCCAGTAAGCAGACTACGGAGCCTCAGCCTAAGGAGGGGACCCAGGTGGTAGCAATCTTCAGTTA TGAGGCTGCCCAGCCAGAAGACCTGGAATTTGTGGAAGGAGATGTAATCCTGGTACTGTCACATGTGAAT GAAGAATGGCTGGAAGGGGAGTGTAAAGGGAAAGTTGGCATTTTCCCGAAGGCTTTTGTTGAAGGATGTG CAGCCAAGAATTTGGAAGGCATTCCCAGAGAAGTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG208432 representing NM_010877
Red=Cloning site Green=Tags(s) MSLAEAIRLWNEGVLAADKKDWKGALEAFSEVQDPHSRICFNIGCVNTILENLQAAEQAFTKSINRDKHS AVAYFQRGMLYYRMEKYDLAIKDLKEALTQLRGNQLIDYKILGLQFKLFACEVLYNIALMHAKKEEWKKA EEQLALATNMKSEPRHSKIDKAMESIWKQKLFEPVVIPVGRLFRPNERQVAQLAKKDYLGKATVVASVVH QDNFSGFAPLQPQSAEPPPRPKTPEIFRALEGEAHRVLFGFVPETPEELQVMPGNIVFVLKKGSDNWATV MFNGQKGLVPCNYLEPVELRIHPQSQPQEDTSPESDIPPPPNSSPPGRLQLSPGHKQKEPKELKLSVPMP YMLKVHYKYTVVMETRLGLPYSQLRNMVSKKLALSPEHTKLSYRRRDSHELLLLSEESMKDAWGQVKNYC LTLWCEHTVGDQGLIDEPIQRENSDASKQTTEPQPKEGTQVVAIFSYEAAQPEDLEFVEGDVILVLSHVN EEWLEGECKGKVGIFPKAFVEGCAAKNLEGIPREV TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_010877 |
| ORF Size | 1575 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_010877.2, NP_035007.1 |
| RefSeq Size | 2597 bp |
| RefSeq ORF | 1578 bp |
| Locus ID | 17970 |
| UniProt ID | O70145 |
| Gene Summary | NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC205584 | Ncf2 (untagged) - Mouse neutrophil cytosolic factor 2 (Ncf2), (10ug) |
CNY 3,920.00 |
|
| MR208432 | Ncf2 (Myc-DDK-tagged) - Mouse neutrophil cytosolic factor 2 (Ncf2) |
CNY 3,920.00 |
|
| MR208432L3 | Lenti ORF clone of Ncf2 (Myc-DDK-tagged) - Mouse neutrophil cytosolic factor 2 (Ncf2) |
CNY 6,460.00 |
|
| MR208432L4 | Lenti ORF clone of Ncf2 (mGFP-tagged) - Mouse neutrophil cytosolic factor 2 (Ncf2) |
CNY 6,460.00 |
