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Hexa (NM_010421) Mouse Tagged ORF Clone
CAT＃: MG208464
- TrueORF®
Hexa (tGFP-tagged) - Mouse hexosaminidase A (Hexa)
ORF Plasmid: DDK
"NM_010421" in other vectors (4)
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CNY 4,940.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Hex-1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG208464 representing NM_010421
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGGCTGCAGGCTCTGGGTTTCGCTGCTGCTGGCGGCGGCGTTGGCTTGCTTGGCCACGGCACTGT GGCCGTGGCCCCAGTACATCCAAACCTACCACCGGCGCTACACCCTGTACCCCAACAACTTCCAGTTCCG GTACCATGTCAGTTCGGCCGCGCAGGCGGGCTGCGTCGTCCTCGACGAGGCCTTTCGACGCTACCGTAAC CTGCTCTTCGGTTCCGGCTCTTGGCCCCGACCCAGCTTCTCAAATAAACAGCAAACGTTGGGGAAGAACA TTCTGGTGGTCTCCGTCGTCACAGCTGAATGTAATGAATTTCCTAATTTGGAGTCGGTAGAAAATTACAC CCTAACCATTAATGATGACCAGTGTTTACTCGCCTCTGAGACTGTCTGGGGCGCTCTCCGAGGTCTGGAG ACTTTCAGTCAGCTTGTTTGGAAATCAGCTGAGGGCACGTTCTTTATCAACAAGACAAAGATTAAAGACT TTCCTCGATTCCCTCACCGGGGCGTACTGCTGGATACATCTCGCCATTACCTGCCATTGTCTAGCATCCT GGATACACTGGATGTCATGGCATACAATAAATTCAACGTGTTCCACTGGCACTTGGTGGACGACTCTTCC TTCCCATATGAGAGCTTCACTTTCCCAGAGCTCACCAGAAAGGGGTCCTTCAACCCTGTCACTCACATCT ACACAGCACAGGATGTGAAGGAGGTCATTGAATACGCAAGGCTTCGGGGTATCCGTGTGCTGGCAGAATT TGACACTCCTGGCCACACTTTGTCCTGGGGGCCAGGTGCCCCTGGGTTATTAACACCTTGCTACTCTGGG TCTCATCTCTCTGGCACATTTGGACCGGTGAACCCCAGTCTCAACAGCACCTATGACTTCATGAGCACAC TCTTCCTGGAGATCAGCTCAGTCTTCCCGGACTTTTATCTCCACCTGGGAGGGGATGAAGTCGACTTCAC CTGCTGGAAGTCCAACCCCAACATCCAGGCCTTCATGAAGAAAAAGGGCTTTACTGACTTCAAGCAGCTG GAGTCCTTCTACATCCAGACGCTGCTGGACATCGTCTCTGATTATGACAAGGGCTATGTGGTGTGGCAGG AGGTATTTGATAATAAAGTGAAGGTTCGGCCAGATACAATCATACAGGTGTGGCGGGAAGAAATGCCAGT AGAGTACATGTTGGAGATGCAAGATATCACCAGGGCTGGCTTCCGGGCCCTGCTGTCTGCTCCCTGGTAC CTGAACCGTGTAAAGTATGGCCCTGACTGGAAGGACATGTACAAAGTGGAGCCCCTGGCGTTTCATGGTA CGCCTGAACAGAAGGCTCTGGTCATTGGAGGGGAGGCCTGTATGTGGGGAGAGTATGTGGACAGCACCAA CCTGGTCCCCAGACTCTGGCCCAGAGCGGGTGCCGTCGCTGAGAGACTGTGGAGCAGTAACCTGACAACT AATATAGACTTTGCCTTTAAACGTTTGTCGCATTTCCGTTGTGAGCTGGTGAGGAGAGGAATCCAGGCCC AGCCCATCAGTGTAGGCTACTGTGAGCAGGAGTTTGAGCAGACT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG208464 representing NM_010421
Red=Cloning site Green=Tags(s) MAGCRLWVSLLLAAALACLATALWPWPQYIQTYHRRYTLYPNNFQFRYHVSSAAQAGCVVLDEAFRRYRN LLFGSGSWPRPSFSNKQQTLGKNILVVSVVTAECNEFPNLESVENYTLTINDDQCLLASETVWGALRGLE TFSQLVWKSAEGTFFINKTKIKDFPRFPHRGVLLDTSRHYLPLSSILDTLDVMAYNKFNVFHWHLVDDSS FPYESFTFPELTRKGSFNPVTHIYTAQDVKEVIEYARLRGIRVLAEFDTPGHTLSWGPGAPGLLTPCYSG SHLSGTFGPVNPSLNSTYDFMSTLFLEISSVFPDFYLHLGGDEVDFTCWKSNPNIQAFMKKKGFTDFKQL ESFYIQTLLDIVSDYDKGYVVWQEVFDNKVKVRPDTIIQVWREEMPVEYMLEMQDITRAGFRALLSAPWY LNRVKYGPDWKDMYKVEPLAFHGTPEQKALVIGGEACMWGEYVDSTNLVPRLWPRAGAVAERLWSSNLTT NIDFAFKRLSHFRCELVRRGIQAQPISVGYCEQEFEQT TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_010421 |
| ORF Size | 1584 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_010421.5 |
| RefSeq Size | 1866 bp |
| RefSeq ORF | 1587 bp |
| Locus ID | 15211 |
| UniProt ID | P29416 |
| Gene Summary | This gene encodes a member of the glycosyl hydrolase 20 family of proteins. The encoded preproprotein is proteolytically processed to generate the alpha subunit of the lysosomal enzyme beta-hexosaminidase. This enzyme, together with the cofactor GM2 activator protein, catalyzes the degradation of the ganglioside GM2, and other molecules containing terminal N-acetyl hexosamines. Mice lacking the encoded protein exhibit accumulation of gangliosides in the brain and membranous cytoplasmic bodies in neurons. Certain mutations in the human ortholog of this gene cause Tay-Sachs disease. [provided by RefSeq, Aug 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC201099 | Hexa (untagged) - Mouse hexosaminidase A (Hexa), (10ug) |
CNY 2,000.00 |
|
| MR208464 | Hexa (Myc-DDK-tagged) - Mouse hexosaminidase A (Hexa) |
CNY 3,936.00 |
|
| MR208464L3 | Lenti ORF clone of Hexa (Myc-DDK-tagged) - Mouse hexosaminidase A (Hexa) |
CNY 6,370.00 |
|
| MR208464L4 | Lenti ORF clone of Hexa (mGFP-tagged) - Mouse hexosaminidase A (Hexa) |
CNY 6,370.00 |
