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Kpna2 (BC003274) Mouse Tagged ORF Clone
CAT＃: MG208477
- TrueORF®
Kpna2 (tGFP-tagged) - Mouse karyopherin (importin) alpha 2 (cDNA clone MGC:6031 IMAGE:3499880)
ORF Plasmid: DDK
"BC003274" in other vectors (3)
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CNY 5,890.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 2410044B12Rik; IPOA1; PTAC58; Rch1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG208477 representing BC003274
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCACGAACGAGAATGCTAACTTACCAGCTGCCCGACTTAACAGGTTCAAGAACAAGGGGAAGGACA GCACAGAAATGCGTCGCCGCCGAATAGAAGTTAATGTGGAACTCAGGAAAGCTAAAAAAGATGAGCAGAT GCTGAAAAGAAGAAACGTCAGCTCCTTTCCTGATGATGCTACTTCTCCGCTACAGGAAAACCGGAACAAC CAGGGTACTGTAAATTGGTCTGTTGAGGACATTGTTAAAGGCATAAACAGTAACAATTTGGAAAGCCAGC TCCAAGCTACTCAAGCTGCTCGGAAATTGCTTTCTAGAGAGAAACAGCCTCCTATAGACAACATCATCCG GGCTGGTTTGATCCCAAAATTTGTGTCCTTCTTGGGCAAAACTGATTGTAGTCCTATTCAGTTTGAGTCT GCTTGGGCACTCACCAACATTGCTTCTGGAACATCTGAACAGACCAAAGCTGTGGTGGATGGAGGTGCTA TCCCAGCGTTTATTTCTCTCTTGGCATCTCCTCATGCTCACATCAGCGAGCAAGCTGTTTGGGCTCTTGG AAACATTGCAGGTGATGGTTCAGCTTTCCGAGACTTAGTTATCAAACACGGTGCGATTGACCCACTGTTG GCACTTCTTGCAGTTCCCGATCTGTCTACCTTGGCATGTGGTTACTTACGTAATCTTACCTGGACGCTTT CAAACCTTTGTCGAAACAAGAACCCTGCACCTCCCTTAGATGCCGTTGAGCAGATTCTTCCTACGTTAGT TCGACTCCTGCACCACAATGATCCAGAAGTATTGGCAGATTCCTGCTGGGCCATTTCCTACCTGACTGAT GGTCCAAATGAGCGCATTGAGATGGTTGTGAAGAAAGGAGTTGTTCCCCAACTTGTGAAGCTTCTAGGAG CTACTGAACTGCCCATTGTGACTCCCGCACTAAGAGCCATAGGGAATATTGTCACTGGAACAGATGAGCA GACTCAGAAAGTGATCGATGCAGGAGCACTTGCAGTCTTTCCCAGCCTGCTAACAAACCCCAAAACTAAT ATTCAGAAGGAGGCCACATGGACAATGTCGAACATTACAGCTGGACGCCAGGACCAGATACAGCAAGTTG TGAATCACGGCCTAGTCCCCTTTCTTGTTGGTGTCCTCTCTAAGGCGGACTTTAAGACACAGAAGGAGGC CGCGTGGGCTATAACCAACTATACCAGCGGTGGGACTGTTGAGCAGATTGTGTATCTCGTTCACTGTGGG ATAATAGAACCTTTGATGAACCTCCTGAGTGCAAAAGATACCAAGATTATTCAGGTTATTCTTGACGCCA TTTCAAATATCTTTCAGGCTGCAGAGAAACTAGGTGAGACAGAAAAGCTTAGTATAATGATTGAAGAGTG TGGAGGCTTGGATAAAATTGAAGCACTACAGAGGCATGAAAACGAGTCTGTATACAAGGCCTCATTGAAC TTAATTGAGAAGTACTTCTCAGTGGAGGAAGAGGAAGATCAAAATGTGGTGCCAGAAACTACCTCTGAAG GCTTCGCCTTCCAAGTTCAGGATGGAGCTCCTGGGACCTTTAACTTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG208477 representing BC003274
Red=Cloning site Green=Tags(s) MSTNENANLPAARLNRFKNKGKDSTEMRRRRIEVNVELRKAKKDEQMLKRRNVSSFPDDATSPLQENRNN QGTVNWSVEDIVKGINSNNLESQLQATQAARKLLSREKQPPIDNIIRAGLIPKFVSFLGKTDCSPIQFES AWALTNIASGTSEQTKAVVDGGAIPAFISLLASPHAHISEQAVWALGNIAGDGSAFRDLVIKHGAIDPLL ALLAVPDLSTLACGYLRNLTWTLSNLCRNKNPAPPLDAVEQILPTLVRLLHHNDPEVLADSCWAISYLTD GPNERIEMVVKKGVVPQLVKLLGATELPIVTPALRAIGNIVTGTDEQTQKVIDAGALAVFPSLLTNPKTN IQKEATWTMSNITAGRQDQIQQVVNHGLVPFLVGVLSKADFKTQKEAAWAITNYTSGGTVEQIVYLVHCG IIEPLMNLLSAKDTKIIQVILDAISNIFQAAEKLGETEKLSIMIEECGGLDKIEALQRHENESVYKASLN LIEKYFSVEEEEDQNVVPETTSEGFAFQVQDGAPGTFNF TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC003274 |
| ORF Size | 1587 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC003274.1 |
| RefSeq Size | 1962 bp |
| RefSeq ORF | 1589 bp |
| Locus ID | 16647 |
| Gene Summary | Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR208477 | Kpna2 (Myc-DDK-tagged) - Mouse karyopherin (importin) alpha 2 (cDNA clone MGC:6031 IMAGE:3499880) |
CNY 5,320.00 |
|
| MR208477L3 | Lenti ORF clone of Kpna2 (Myc-DDK-tagged) - Mouse karyopherin (importin) alpha 2 (cDNA clone MGC:6031 IMAGE:3499880) |
CNY 7,220.00 |
|
| MR208477L4 | Lenti ORF clone of Kpna2 (mGFP-tagged) - Mouse karyopherin (importin) alpha 2 (cDNA clone MGC:6031 IMAGE:3499880) |
CNY 7,220.00 |
