United States
Germany
Japan
United Kingdom
China
Ehd1 (NM_010119) Mouse Tagged ORF Clone
CAT＃: MG208555
- TrueORF®
Ehd1 (tGFP-tagged) - Mouse EH-domain containing 1 (Ehd1)
ORF Plasmid: DDK
"NM_010119" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 5,040.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AA409636; Past1; RME-1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG208555 representing NM_010119
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCAGCTGGGTGAGCAAGGATGCCCGCCGCAAGAAGGAGCCGGAGCTCTTCCAGACGGTGGCCGAGG GGCTGCGGCAGCTGTACGCGCAGAAGCTGCTGCCGCTGGAGGAGCACTATCGCTTCCACGAGTTCCACTC GCCCGCGCTGGAGGACGCTGACTTCGACAACAAGCCGATGGTGCTCCTGGTCGGCCAGTACAGCACCGGC AAGACCACCTTCATCCGCCACCTGATCGAGCAGGACTTCCCGGGGATGCGCATCGGGCCGGAGCCCACCA CCGACTCCTTCATCGCGGTCATGCACGGCCCCACCGAGGGCGTGGTGCCCGGCAACGCGCTCGTCGTGGA CCCGCGGCGCCCCTTCCGCAAGCTCAACGCCTTCGGCAACGCCTTCCTCAACAGGTTCATGTGTGCACAG CTGCCCAACCCAGTACTGGACAGCATCAGCATCATTGACACTCCTGGGATCCTGTCTGGGGAGAAGCAGC GCATCAGCCGAGGTTATGACTTTGCGGCTGTCCTTGAGTGGTTCGCAGAGCGTGTGGACCGCATCATCTT GTTGTTCGACGCCCACAAGCTGGACATCTCAGACGAGTTCTCAGAAGTCATCAAGGCCCTCAAAAATCAC GAGGACAAGATCCGTGTGGTGCTGAACAAGGCTGATCAGATCGAGACGCAGCAGCTGATGCGAGTATACG GGGCCCTCATGTGGTCCCTGGGGAAGATCATCAACACCCCCGAGGTGGTCAGAGTCTACATCGGCTCCTT CTGGTCACACCCACTGCTCATCCCTGACAACCGGAAGCTCTTCGAGGCAGAGGAGCAGGACCTCTTCAAA GACATCCAGTCTCTGCCGAGAAACGCCGCCCTCAGGAAGCTCAATGACCTCATCAAGCGGGCCAGGCTGG CCAAGGTCCATGCCTACATCATCAGCTCCCTCAAGAAGGAGATGCCCAATGTTTTCGGGAAAGAGAGCAA GAAGAAAGAGCTGGTGAACAACCTGGGAGAGATCTACCAGAAGATCGAGCGGGAGCACCAGATCTCCTCC GGCGACTTCCCAAGCCTGCGTAAGATGCAGGAACTCCTGCAGACCCAGGACTTCAGCAAGTTCCAGGCCT TGAAGCCCAAGCTGCTGGATACAGTGGATGATATGCTGGCCAACGATATAGCTCGGCTGATGGTGATGGT GCGCCAGGAGGAGTCCCTGATGCCCTCACAGGCTGTGAAGGGTGGTGCTTTTGATGGCACCATGAATGGG CCCTTTGGGCATGGCTACGGCGAGGGGGCTGGCGAGGGCATTGATGATGTTGAGTGGGTAGTTGGCAAGG ACAAGCCCACCTATGATGAGATCTTCTACACACTGTCTCCTGTCAACGGCAAGATCACAGGTGCTAATGC CAAGAAGGAGATGGTGAAGTCCAAGCTGCCCAACACAGTGCTGGGGAAGATCTGGAAGTTGGCAGATGTG GACAAGGATGGCCTGCTGGATGACGAGGAGTTTGCCCTGGCCAACCACCTTATCAAGGTGAAGCTAGAGG GCCACGAGCTGCCCGCTGACCTTCCTCCACATCTCATTCCACCCTCCAAACGGAGGCACGAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG208555 representing NM_010119
Red=Cloning site Green=Tags(s) MFSWVSKDARRKKEPELFQTVAEGLRQLYAQKLLPLEEHYRFHEFHSPALEDADFDNKPMVLLVGQYSTG KTTFIRHLIEQDFPGMRIGPEPTTDSFIAVMHGPTEGVVPGNALVVDPRRPFRKLNAFGNAFLNRFMCAQ LPNPVLDSISIIDTPGILSGEKQRISRGYDFAAVLEWFAERVDRIILLFDAHKLDISDEFSEVIKALKNH EDKIRVVLNKADQIETQQLMRVYGALMWSLGKIINTPEVVRVYIGSFWSHPLLIPDNRKLFEAEEQDLFK DIQSLPRNAALRKLNDLIKRARLAKVHAYIISSLKKEMPNVFGKESKKKELVNNLGEIYQKIEREHQISS GDFPSLRKMQELLQTQDFSKFQALKPKLLDTVDDMLANDIARLMVMVRQEESLMPSQAVKGGAFDGTMNG PFGHGYGEGAGEGIDDVEWVVGKDKPTYDEIFYTLSPVNGKITGANAKKEMVKSKLPNTVLGKIWKLADV DKDGLLDDEEFALANHLIKVKLEGHELPADLPPHLIPPSKRRHE TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_010119 |
| ORF Size | 1602 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_010119.5, NP_034249.1 |
| RefSeq Size | 3182 bp |
| RefSeq ORF | 1605 bp |
| Locus ID | 13660 |
| UniProt ID | Q9WVK4 |
| Gene Summary | ATP- and membrane-binding protein that controls membrane reorganization/tubulation upon ATP hydrolysis. In vitro causes vesiculation of endocytic membranes (By similarity). Acts in early endocytic membrane fusion and membrane trafficking of recycling endosomes (PubMed:15930129, PubMed:20159556). Recruited to endosomal membranes upon nerve growth factor stimulation, indirectly regulates neurite outgrowth (By similarity). Plays a role in myoblast fusion (PubMed:21177873). Involved in the unidirectional retrograde dendritic transport of endocytosed BACE1 and in efficient sorting of BACE1 to axons implicating a function in neuronal APP processing (PubMed:24373286). Plays a role in the formation of the ciliary vesicle (CV), an early step in cilium biogenesis. Proposed to be required for the fusion of distal appendage vesicles (DAVs) to form the CV by recruiting SNARE complex component SNAP29. Is required for recruitment of transition zone proteins CEP290, RPGRIP1L, TMEM67 and B9D2, and of IFT20 following DAV reorganization before Rab8-dependent ciliary membrane extension. Required for the loss of CCP110 form the mother centriole essential for the maturation of the basal body during ciliogenesis (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC217561 | Ehd1 (untagged) - Mouse EH-domain containing 1 (Ehd1), (10ug) |
CNY 6,840.00 |
|
| MR208555 | Ehd1 (Myc-DDK-tagged) - Mouse EH-domain containing 1 (Ehd1) |
CNY 3,976.00 |
|
| MR208555L3 | Lenti ORF clone of Ehd1 (Myc-DDK-tagged) - Mouse EH-domain containing 1 (Ehd1) |
CNY 6,460.00 |
|
| MR208555L4 | Lenti ORF clone of Ehd1 (mGFP-tagged) - Mouse EH-domain containing 1 (Ehd1) |
CNY 6,376.00 |
