Socs5 (BC053015) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	1810018L08Rik; Cish5; mKIAA0671
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG208589 representing BC053015 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATAAAGTGGGGAAAATGTGGAACAACTTAAAATACAGATGCCAGAATCTCTTCAGCCACGAGGGAG GAAGCCGTAATGAGAACGTGGAGATGAACCCCAACAGATGTCCGTCTGTCAAAGAGAAAAGCATCAGTCT GGGAGAGGCAGCTCCCCAGCAAGAGAGCAGTCCCTTAAGAGAAAATGTTGCCTTACAGCTGGGACTGAGC CCTTCCAAGACCTTTTCCAGGCGGAACCAAAACTGTGCCGCAGAGATCCCTCAAGTGGTTGAAATCAGCA TCGAGAAAGACAGTGACTCGGGTGCCACCCCAGGAACGAGGCTTGCACGGAGAGACTCCTACTCGCGGCA CGCCCCGTGGGGAGGAAAGAAGAAACATTCCTGTTCCACAAAGACCCAGAGTTCATTGGATACCGAGAAA AAGTTTGGTAGAACTCGAAGCGGCCTTCAGAGGCGAGAGCGGCGCTATGGAGTCAGCTCCATGCAGGACA TGGACAGCGTTTCTAGCCGCGCGGTCGGGAGCCGCTCCCTGAGGCAGAGGCTCCAGGACACGGTGGGTTT GTGTTTTCCCATGAGAACTTACAGCAAGCAGTCAAAGCCACTCTTTTCCAATAAAAGAAAAATACATCTT TCTGAATTAATGCTGGAGAAATGCCCTTTTCCTGCTGGCTCGGATTTAGCACAAAAGTGGCATTTGATTA AACAGCATACCGCCCCTGTGAGCCCACACTCAACATTTTTTGATACATTTGATCCATCACTGGTGTCTAC AGAAGATGAAGAAGATAGGCTTCGCGAGAGAAGACGGCTTAGTATCGAAGAAGGGGTGGATCCCCCTCCC AACGCACAAATACACACCTTTGAAGCTACTGCACAGGTCAACCCATTGTATAAGCTGGGACCAAAGTTAG CTCCTGGGATGACAGAGATAAGTGGAGATGGTTCTGCAATTCCACAAACGAATTGTGACTCAGAAGAGGA TTCAACCACCCTATGTCTGCAGTCACGGAGGCAGAAGCAGCGCCAGGTGTCCGGGGACAGCCACGCGCAC GTTAGCAGACAGGGAGCTTGGAAAGTTCATACGCAGATCGATTACATACACTGCCTCGTGCCAGATTTGC TTCAGATCACAGGGAATCCCTGTTACTGGGGCGTGATGGACCGATACGAGGCCGAAGCCCTTCTAGAAGG GAAACCGGAAGGCACGTTCTTGCTCAGGGACTCTGCACAGGAGGACTACCTCTTCTCTGTGAGCTTCCGC CGCTACAACAGGTCTCTGCACGCCCGGATCGAGCAGTGGAACCACAACTTCAGCTTCGATGCCCATGACC CCTGCGTGTTTCACTCCTCCACTGTCACGGGGCTTCTCGAACACTATAAAGACCCCAGCTCTTGCATGTT TTTTGAACCGTTGCTAACGATATCACTGAATAGAACTTTCCCTTTCAGCCTGCAGTATATCTGCCGCGCA GTGATCTGCAGATGCACTACGTATGATGGGATTGACGGGCTCCCGCTACCGTCGATGTTACAGGATTTTT TAAAAGAGTATCATTATAAACAAAAAGTTAGGGTTCGCTGGTTAGAACGAGAACCAGTCAAAGCAAAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG208589 representing BC053015 Red=Cloning site Green=Tags(s) MDKVGKMWNNLKYRCQNLFSHEGGSRNENVEMNPNRCPSVKEKSISLGEAAPQQESSPLRENVALQLGLS PSKTFSRRNQNCAAEIPQVVEISIEKDSDSGATPGTRLARRDSYSRHAPWGGKKKHSCSTKTQSSLDTEK KFGRTRSGLQRRERRYGVSSMQDMDSVSSRAVGSRSLRQRLQDTVGLCFPMRTYSKQSKPLFSNKRKIHL SELMLEKCPFPAGSDLAQKWHLIKQHTAPVSPHSTFFDTFDPSLVSTEDEEDRLRERRRLSIEEGVDPPP NAQIHTFEATAQVNPLYKLGPKLAPGMTEISGDGSAIPQTNCDSEEDSTTLCLQSRRQKQRQVSGDSHAH VSRQGAWKVHTQIDYIHCLVPDLLQITGNPCYWGVMDRYEAEALLEGKPEGTFLLRDSAQEDYLFSVSFR RYNRSLHARIEQWNHNFSFDAHDPCVFHSSTVTGLLEHYKDPSSCMFFEPLLTISLNRTFPFSLQYICRA VICRCTTYDGIDGLPLPSMLQDFLKEYHYKQKVRVRWLEREPVKAK TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC053015
ORF Size	1608 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC053015, AAH53015
RefSeq Size	4460 bp
RefSeq ORF	1610 bp
Locus ID	56468
Gene Summary	SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. May be a substrate-recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Inhibits for instance EGF signaling by mediating the degradation of the EGF receptor/EGFR. Involved in the regulation of T-helper cell differentiation by inhibiting of the IL4 signaling pathway which promotes differentiation into the Th2 phenotype. Can also partially inhibit IL6 and LIF signaling.[UniProtKB/Swiss-Prot Function]

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