Cdyl (BC062123) Mouse Tagged ORF Clone

CAT#: MG208673

  • TrueORF®

Cdyl (tGFP-tagged) - Mouse chromodomain protein, Y chromosome-like (cDNA clone MGC:69916 IMAGE:6826210)



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Mouse Protein / Lysate Request

CNY 5,040.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms AI325931
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG208673 representing BC062123
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCTCCGAGGAGCTGTACGAGGTGGAAAGTATCGTTGACAAAAGGAAAAACAAGAAAGGGAAGACAG
AATATCTGGTGCGGTGGAAAGGCTATGACAGTGAGGATGACACGTGGGAGCCTGAGCAGCACCTGGTGAA
CTGTGAGGAATACATCCATGACTTCAACCGGCGCCACAACGAGAGGCAAAAGGAAGGTAGCCTGGCTCGT
GCCAGCAGAGCCTCCCCCAGCAACGCCCGGAAGCAGATTTCCAGGTCCACCCACAGCACTCTCTCCAAGA
CCAACTCCAAAGCACTTGTGGTAGGCAAAGATCATGAGTCCAAAAGCAGCCAGCTGTTGGCTGCCAGCCA
GAAGTTCAGGAAAAACCCAGCCCCATCTCTTGCAAACCGCAAGAACATGGACCTCGCCAAGTCAGGGATC
AAAATTCTCGTGCCTAAGAGCCCCGTTAAGGGCAGGACCTCGGTTGATGGCTTTCAGGGGGAGAGCCCCG
AGAAGCTGGACCCTGTGGATCAGGGTGCCGAGGACACTGTAGCCCCAGAGGTGACTGCAGAGAAGCCCAC
TGGGGCTTTGCTGGGCCCTGGTGCGGAGCGAGCCAGGATGGGGAGCAGGCCCCGAATACATCCACTAGTG
CCTCAGGTTTCTGGCCCCGTGACTGCTGCCATGGCCACAGGCTTAGCTGTTAATGGAAAAGGTACATCTC
CATTCATGGATGCGCTAGCAGCCAACGGAACAGTCACCATACAGACATCCGTAACAGGAGTGACAGCCGG
GAAAAGGAAATTTATTGACGACAGAAGAGACCAACCTTTTGACAAGCGGTTGCGTTTCAGTGTGAGGCAG
ACAGAGAGTGCCTACAGATACAGAGATATTGTCGTCAGGAAGCAAGATGGCTTCACCCACATCTTGTTAT
CCACAAAATCGTCAGAGAATAACTCACTAAACCCAGAGGTGATGAAAGAAGTGCAGAGCGCCCTGAGCAC
AGCTGCAGCCGACGACAGCAAGCTGGTTCTGCTCAGCGCCGTGGGCAGCGTCTTCTGCTGTGGTCTGGAC
TTTATTTATTTTATTCGGCGCCTCACAGATGACCGAAAGAGAGAAAGCACTAAAATGGCAGACGCTATCA
GAAACTTCGTGAATACTTTCATTCAGTTTAAGAAGCCTATTATTGTAGCTGTTAATGGCCCAGCCATTGG
ACTAGGAGCATCCATATTGCCTCTTTGTGATGTGGTTTGGGCTAACGAAAAGGCTTGGTTTCAAACACCC
TATACCACCTTCGGACAGAGTCCAGATGGCTGCTCTACCGTTATGTTTCCCAAGATTATGGGAGGAGCAT
CTGCGAATGAAATGCTGTTCAGTGGGCGGAAGTTGACGGCACAGGAGGCCTGTGGCAAGGGTCTGGTCTC
CCAGGTGTTTTGGCCAGGAACCTTCACACAGGAAGTCATGGTTCGAATCAAGGAGCTGGCTTCATGTAAC
CCAGTTGTCCTGGAGGAATCCAAAGCCCTGGTGCGCTGCAATATGAAGATGGAGCTAGAGCAGGCCAATG
AGAGAGAATGTGAAGTGCTGAAGAAGATCTGGGGCTCCGCCCAGGGCATGGACTCCATGTTAAAGTACTT
ACAGAGGAAAATCGATGAGTTC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG208673 representing BC062123
Red=Cloning site Green=Tags(s)

MASEELYEVESIVDKRKNKKGKTEYLVRWKGYDSEDDTWEPEQHLVNCEEYIHDFNRRHNERQKEGSLAR
ASRASPSNARKQISRSTHSTLSKTNSKALVVGKDHESKSSQLLAASQKFRKNPAPSLANRKNMDLAKSGI
KILVPKSPVKGRTSVDGFQGESPEKLDPVDQGAEDTVAPEVTAEKPTGALLGPGAERARMGSRPRIHPLV
PQVSGPVTAAMATGLAVNGKGTSPFMDALAANGTVTIQTSVTGVTAGKRKFIDDRRDQPFDKRLRFSVRQ
TESAYRYRDIVVRKQDGFTHILLSTKSSENNSLNPEVMKEVQSALSTAAADDSKLVLLSAVGSVFCCGLD
FIYFIRRLTDDRKRESTKMADAIRNFVNTFIQFKKPIIVAVNGPAIGLGASILPLCDVVWANEKAWFQTP
YTTFGQSPDGCSTVMFPKIMGGASANEMLFSGRKLTAQEACGKGLVSQVFWPGTFTQEVMVRIKELASCN
PVVLEESKALVRCNMKMELEQANERECEVLKKIWGSAQGMDSMLKYLQRKIDEF

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC062123
ORF Size 1634 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC062123, AAH62123
RefSeq Size 3596 bp
RefSeq ORF 1634 bp
Locus ID 12593
Gene Summary Isoform 2: Chromatin reader protein that recognizes and binds histone H3 trimethylated at 'Lys-9', dimethylated at 'Lys-27' and trimethylated at 'Lys-27' (H3K9me3, H3K27me2 and H3K27me3, respectively) (PubMed:12947414). Part of multimeric repressive chromatin complexes, where it is required for transmission and restoration of repressive histone marks, thereby preserving the epigenetic landscape (PubMed:12947414). Required for chromatin targeting and maximal enzymatic activity of Polycomb repressive complex 2 (PRC2); acts as a positive regulator of PRC2 activity by bridging the pre-existing histone H3K27me3 and newly recruited PRC2 on neighboring nucleosomes (By similarity). Acts as a corepressor for REST by facilitating histone-lysine N-methyltransferase EHMT2 recruitment and H3K9 dimethylation at REST target genes for repression (By similarity). Involved X chromosome inactivation in females: recruited to Xist RNA-coated X chromosome and facilitates propagation of H3K9me2 by anchoring EHMT2 (PubMed:24144980). Required for neuronal migration during brain development by repressing expression of RHOA (PubMed:28076783). In addition to act as a chromatin reader, acts as a hydro-lyase (By similarity). Shows crotonyl-coA hydratase activity by mediating the conversion of crotonyl-CoA ((2E)-butenoyl-CoA) to beta-hydroxybutyryl-CoA (3-hydroxybutanoyl-CoA), thereby acting as a negative regulator of histone crotonylation (By similarity). Histone crotonylation is required during spermatogenesis; down-regulation of histone crotonylation by CDYL regulates the reactivation of sex chromosome-linked genes in round spermatids and histone replacement in elongating spermatids (PubMed:28803779). May have histone acetyltransferase activity; such activity is however unsure in vivo (PubMed:12072557).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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