Camk2b (BC080273) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG208679 representing BC080273 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCACCACGGTGACCTGCACCCGTTTCACCGACGAGTACCAGCTATACGAGGATATTGGCAAGGGGG CTTTCTCTGTGGTCCGACGCTGTGTCAAGCTCTGTACCGGCCATGAGTATGCAGCCAAGATCATTAATAC CAAGAAGCTGTCGGCCAGAGATCACCAGAAACTGGAGAGAGAAGCTCGGATCTGCCGCCTGCTGAAGCAT TCCAACATTGTACGCCTCCATGACAGCATCTCTGAAGAGGGCTTCCACTACCTGGTCTTCGATCTGGTCA CTGGTGGGGAGCTCTTTGAAGACATCGTGGCAAGAGAGTACTACAGTGAAGCTGATGCCAGTCACTGCAT CCAGCAGATCCTGGAAGCTGTTCTCCATTGTCACCAAATGGGGGTCGTCCACAGAGACCTCAAGCCTGAG AACCTGCTTCTGGCCAGCAAATGCAAAGGCGCCGCAGTGAAGCTGGCAGACTTCGGCCTGGCCATCGAGG TTCAGGGAGACCAGCAGGCATGGTTTGGATTTGCGGGAACGCCAGGCTACCTGTCTCCCGAGGTCCTTCG GAAGGAGGCCTACGGCAAACCTGTGGACATCTGGGCATGTGGGGTGATCCTGTATATCCTGCTGGTGGGC TACCCACCTTTCTGGGATGAGGACCAACACAAGCTGTACCAGCAGATCAAGGCTGGGGCGTATGATTTCC CATCCCCTGAGTGGGACACCGTTACTCCTGAAGCCAAAAACCTCATCAACCAGATGCTGACCATCAACCC TGCCAAGCGCATCACGGCCCATGAGGCCCTGAAGCACCCATGGGTCTGCCAACGTTCCACCGTGGCCTCT ATGATGCACAGACAGGAGACTGTGGAATGTCTGAAGAAGTTCAATGCAAGGAGGAAGCTCAAGGGAGCCA TCCTCACCACTATGCTGGCCACACGGAATTTCTCAGCCAAGAGTTTACTCAACAAGAAAGCAGATGGAGT CAAGCCCCAGACAAACAGCACCAAAAACAGCTCGGCCATCACCAGCCCCAAAGGATCTCTCCCTCCTGCT GCCCTGGAATCTTCCGACAGCACCAACACAACCATAGAGGATGAAGATGCCAAAGCCCCCAGGATCTCTG ACATCCTCAACTCAGTGAGGCGGGGCTCAGGGACCCCAGAAGCTGAGGGCCTCCCACCAGTGGGCCCCCC ACCCTGCCCATCTCCGACTCTCCCTGGCCCCTTGCCCACCCCATCCCGGAAGCAGGAAATCATCAAGACC ACAGAGCAGCTCATTGAGGCCGTCAACAATGGGGACTTTGAGGCCTATGCGAAAATCTGTGACCCAGGCC TGACCTCATTTGAGCCTGAAGCTCTGGGCAACCTGGTCGAAGGGATGGATTTCCACAGATTCTACTTTGA GAACCTGCTGGCCAAGAACAGCAAGCCGATCCACACCACCATCCTGAACCCGCACGTGCACGTCATTGGC GAGGATGCGGCATGCATCGCCTACATCCGCCTCACACAGTACATCGATGGCCAGGGCAGACCCCGTACCA GCCAGTCCGAAGAGACCCGTGTGTGGCACCGCCGCGACGGCAAGTGGCAGAATGTACATTTCCACTGCTC GGGCGCTCCAGTGGCCCCGCTGCAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG208679 representing BC080273 Red=Cloning site Green=Tags(s) MATTVTCTRFTDEYQLYEDIGKGAFSVVRRCVKLCTGHEYAAKIINTKKLSARDHQKLEREARICRLLKH SNIVRLHDSISEEGFHYLVFDLVTGGELFEDIVAREYYSEADASHCIQQILEAVLHCHQMGVVHRDLKPE NLLLASKCKGAAVKLADFGLAIEVQGDQQAWFGFAGTPGYLSPEVLRKEAYGKPVDIWACGVILYILLVG YPPFWDEDQHKLYQQIKAGAYDFPSPEWDTVTPEAKNLINQMLTINPAKRITAHEALKHPWVCQRSTVAS MMHRQETVECLKKFNARRKLKGAILTTMLATRNFSAKSLLNKKADGVKPQTNSTKNSSAITSPKGSLPPA ALESSDSTNTTIEDEDAKAPRISDILNSVRRGSGTPEAEGLPPVGPPPCPSPTLPGPLPTPSRKQEIIKT TEQLIEAVNNGDFEAYAKICDPGLTSFEPEALGNLVEGMDFHRFYFENLLAKNSKPIHTTILNPHVHVIG EDAACIAYIRLTQYIDGQGRPRTSQSEETRVWHRRDGKWQNVHFHCSGAPVAPLQ TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC080273
ORF Size	1635 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC080273, AAH80273
RefSeq Size	3827 bp
RefSeq ORF	1637 bp
Locus ID	12323
Gene Summary	Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation, and is involved in dendritic spine and synapse formation, neuronal plasticity and regulation of sarcoplasmic reticulum Ca(2+) transport in skeletal muscle. In neurons, plays an essential structural role in the reorganization of the actin cytoskeleton during plasticity by binding and bundling actin filaments in a kinase-independent manner. This structural function is required for correct targeting of CaMK2A, which acts downstream of NMDAR to promote dendritic spine and synapse formation and maintain synaptic plasticity which enables long-term potentiation (LTP) and hippocampus-dependent learning. In developing hippocampal neurons, promotes arborization of the dendritic tree and in mature neurons, promotes dendritic remodeling. Also regulates the migration of developing neurons (PubMed:29100089). Participates in the modulation of skeletal muscle function in response to exercise. In slow-twitch muscles, is involved in regulation of sarcoplasmic reticulum (SR) Ca(2+) transport and in fast-twitch muscle participates in the control of Ca(2+) release from the SR through phosphorylation of triadin, a ryanodine receptor-coupling factor, and phospholamban (PLN/PLB), an endogenous inhibitor of SERCA2A/ATP2A2 (PubMed:21752990).[UniProtKB/Swiss-Prot Function]

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