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Mus81 (NM_027877) Mouse Tagged ORF Clone
CAT＃: MG208756
- TrueORF®
Mus81 (tGFP-tagged) - Mouse MUS81 endonuclease homolog (yeast) (Mus81)
ORF Plasmid: DDK
"NM_027877" in other vectors (4)
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CNY 5,040.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 1200008A18Rik; AI182501; AW045863 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG208756 representing NM_027877
Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGGCGCGCCC ATGGCGGAGCCGGTCCGCCTGGGCCGGAAGCGTCCGCTGCCCGTTTGCCCCAACCCGCTCTTCGTTCGTT GGCTGACCGAGTGGCGGGACGAGGCAGCCAGCAGGGGGCGCCACACGCGTTTCGTGTTTCAAAAGGCATT GCGCTCCCTCCAACGGTACCCGCTACCATTGCGCAGCGGGAAGGAAGCCAAGATACTCCAGCACTTCGGA GACAGGCTCTGCCGCATGCTGGACGAAAAGCTGAAGCAGCACCTAGCATCAGGCGGTGACCATGCCCCCA GCTCACCATCTGGAAAGAAGGGAGCCAGCAAAGGGCCACCTGAGCAAGTCCAGGACTCTTCCATGCCAGT TCCCACCCAGCCTCAAGCAGGAAGCACCAGTGTTGGCTATTGGCCAGCTCAGAACTCAGGTGCTCGAGAG ATCCTGCTGCAACTCTACAGGGAGCACCTGAATTCTGATGGCCACAGCTTCCTAACCAAAGAGGAGCTGC TGCAGAAGTGTGCCCAGAAGACCCCCAGGGTAGTGCCTGGAAGTTCGAAACCCTGGCCTGCCCTCCGGAG CCTCCTCCATAGGAACCTCATCCTTGGAACGCATCGGCCAGCCAGGTATGCACTCACACCAGAGGGTCTG GAGCTGGCTCAGAAGCTGGCCGAGGCGGAAGGCCTGAGCACTCGGCACGCTGGCTTTAGGCCAGAGGAAC ATCACGGAGAGGACTCAGCAGTTCCAGAAGCCTTGTCAGAACCTGGCACCACCGAGGGGGCCGTCCAGCA GAGACCACTGGAGCTAAGGCCTAGCGAGTACAGAGTGCTGTTGTGTGTGGACATTGGCGAAACCAGAGGG GCAGGACACAGGCCAGAAATGCTCCGAGAGTTACAAAGGCTGCGTGTGCCCCACACCGTACGCAAGCTAC ACGTTGGAGACTTTGTGTGGGTGGCACAGGAGACCAGGCCCAGAGACCCAGAAAGACCTGGGGAGCTGGT CCTGGACCACATTGTGGAACGCAAGCGGCTAGATGACCTATGCAGCAGCATCATTGACGGCCGCTTTCGG GAGCAGAAGTTCCGCCTGAAGCGCTGTGGCCTGGGGCACCGGGTATACTTAGTGGAAGAACATGGGTCTG TCCACAACCTTAGCCTTCCCGAGAGCACCTTGCTGCAGGCTGTCACAAACACCCAGGTCATTGATGGCTT TTTTGTGAAGCGAACCATGGATATTAAGGAGTCGGCTGGCTACCTGGCGCTTTTGACAAAGGGCCTGGAA AGACTGTACCAGGGCCACACCCTACGCAGCCGCCCTTGGGGGGCCCCAGGGGCTGCTGAATCAGAAGCAA AGCCTTCCACAAACCCTCTCTGCTCACTCCTCACCTTCAGTGACTTCAATGCAGAAGCTGTCAAGAACAA GGCCCAGTCTGTGCGAGAAGTATTTGCCCGGCAGCTGATGCAGGTGCGTGGACTGAGTGGGGAGAAGGCA GCAGCCGTGGTGGATCGATACAGCACCCCTGCCAGTCTCCTGGCTGCTTATGATGCCTGTGCCACCGCGA AGGAGCAGGAGATGCTCTTGAGCACCATCAAGTGTGGGCGTCTGCAGAGGAATCTGGGACCCGCTCTGAG CAGGACCCTGTACCAGTTGTACTGCAGCCACAGCCCTCTGAGC ACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG208756 representing NM_027877
Red=Cloning site Green=Tags(s) MAEPVRLGRKRPLPVCPNPLFVRWLTEWRDEAASRGRHTRFVFQKALRSLQRYPLPLRSGKEAKILQHFG DRLCRMLDEKLKQHLASGGDHAPSSPSGKKGASKGPPEQVQDSSMPVPTQPQAGSTSVGYWPAQNSGARE ILLQLYREHLNSDGHSFLTKEELLQKCAQKTPRVVPGSSKPWPALRSLLHRNLILGTHRPARYALTPEGL ELAQKLAEAEGLSTRHAGFRPEEHHGEDSAVPEALSEPGTTEGAVQQRPLELRPSEYRVLLCVDIGETRG AGHRPEMLRELQRLRVPHTVRKLHVGDFVWVAQETRPRDPERPGELVLDHIVERKRLDDLCSSIIDGRFR EQKFRLKRCGLGHRVYLVEEHGSVHNLSLPESTLLQAVTNTQVIDGFFVKRTMDIKESAGYLALLTKGLE RLYQGHTLRSRPWGAPGAAESEAKPSTNPLCSLLTFSDFNAEAVKNKAQSVREVFARQLMQVRGLSGEKA AAVVDRYSTPASLLAAYDACATAKEQEMLLSTIKCGRLQRNLGPALSRTLYQLYCSHSPLS TRPLE - GFP Tag - V |
| Restriction Sites |
AscI-NotI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_027877 |
| ORF Size | 1653 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_027877.4 |
| RefSeq Size | 2004 bp |
| RefSeq ORF | 1656 bp |
| Locus ID | 71711 |
| UniProt ID | Q91ZJ0 |
| Gene Summary | Interacts with EME1 and EME2 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC201849 | Mus81 (untagged) - Mouse MUS81 endonuclease homolog (yeast) (Mus81), (10ug) |
CNY 2,000.00 |
|
| MR208756 | Mus81 (Myc-DDK-tagged) - Mouse MUS81 endonuclease homolog (yeast) (Mus81) |
CNY 4,104.00 |
|
| MR208756L3 | Lenti ORF clone of Mus81 (Myc-DDK-tagged) - Mouse MUS81 endonuclease homolog (yeast) (Mus81) |
CNY 6,460.00 |
|
| MR208756L4 | Lenti ORF clone of Mus81 (mGFP-tagged) - Mouse MUS81 endonuclease homolog (yeast) (Mus81) |
CNY 6,460.00 |
