Tbccd1 (BC082558) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	5730478M09Rik; AU015083; BB165076
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG208767 representing BC082558 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATCAGTCCGGAGTTCTCCTCTGGGTGAAGGCAGAACCTTTTCTTGTGGGTGCCTTGCAGGAGCCAC CTCCGTCCAAATTCAGCCTTCACTACCTTAGAAAGATAGCCACCTATGTGCGAACTCGGGCCACAGAGGG CGCCTACCCACACCTGTACTGGCCTACCTGGAGGCACATTGCGTGCGGAAAGCTGCAGTTGGCTAAGGAT CTAGCGTGGCTTTACTTTGAAATGTTTGATAATCTTTCTGTGAGGACACCTGAAGAACGCCTGGAATGGT CTGAAATTTTGTCCAACTGCGCAACCGAAGAGGAAGTCGAAAAGCAAAGAAGTCAGCTTTATGTGGACAC GCTGCAATTCCTGCTCTTTCTGTATATCCAGCAGTTAAACAGGATCTCTCTGCGGACATCATTGATTGGG GAAGAATGGCCCAGTCGCAGAAGCAGACCTCAGTCTCCTAGCCCGGCTGAAAGGCCCAGTTGTCACAATA AGAATTGGAATGACTACAGTCACCAGGCTTTTGTCTGTAATCATCTGTCAGAGCTCCTCGAGCTGCTTCT AGACCCAGAGCAGCTCACTGCCTCCTTTCACTCGACCCACAGCAGTCTGCTCTCCCGGGATGCTGTCACG GCGCTCAGCTTTCTCATTGAAGGCACCGTGAGCAGAGCCAAGAAGGTCTATCCCCTTCATCAACTTGCAC TGTGGCAACCACTGCACGTAGCAAGTGGCTTCTCAAAGAGCTCTAAGACTTTCTCTCTCTACAAGCTGGA AGCATGGTTGAGGGCCTCTTTGACCACAAATCCGTTTGGTCCATCTGCTTGCCTCAAGTCTGGAAAGAAA TTGGCTTGGGCTCACCAAGTTGAAGGTTCGACCAAAAGAGCCAAGATTGCTTGCAATGCTCACATGGCTC CTCGGTTGCACCGCATCGTGGTGATGAGCCAGGTTTACAAGCAGACACTGGCCAAGAGCTCAGAAACTCT AGTAGGAGCACATGTCAGGGCTCATCGCTGCAGTGAGTCTTTTATATATCTGCTCTCCCCCTTACGATCC ATGACAATTGAAAAATGCAGGAATAGTACCTTTGTGTTGGGTCCTGTGGAGACGACTCTTCACCTACATG ACTGTGAGAATCTAAAAGTCATTGCTGTTTGCCACCGACTGTCCATCTCTTCTACAACAAGCTGCACCTT TCACATTATGACACCTTCACGTCCACTTATTCTCTCTGGAAACCAGACAGTAACTTTTGCCCCCTTTCAT ACCCATTACCCAATGCTGGAGGACCACATGGCCAGGACTGGCCTTGCTACAGTGCCTAACTATTGGGATA ACCCGATGGTGGTGTGCAAAGAAGGCTCTGTCACAAGTCTCTTCCGGCTCTTGCCTCCTTCTGAGTTCTA CATATTTGTTATTCCCTTTGAGATGGAAGGGGACACAGCAGAGATACCTGGGGGTCTCCCCTCTGCCTAC CAGAAAGCACTGGCTCAAAGAGAAGAGAGCATACATATCTGGCAGAAAACTGTGAAGGAGGCTCGTTTGA CAAAGGAGCAGAGGAAGCAGTTCCAGGCCCTAGTGGAGAACAAGTTCTATGAGTGGTTGGTTAGCACCGG ACATCGGCAGCAGCTGGACAGCCTTGTGCCCACTCCAGCAGCCTCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG208767 representing BC082558 Red=Cloning site Green=Tags(s) MDQSGVLLWVKAEPFLVGALQEPPPSKFSLHYLRKIATYVRTRATEGAYPHLYWPTWRHIACGKLQLAKD LAWLYFEMFDNLSVRTPEERLEWSEILSNCATEEEVEKQRSQLYVDTLQFLLFLYIQQLNRISLRTSLIG EEWPSRRSRPQSPSPAERPSCHNKNWNDYSHQAFVCNHLSELLELLLDPEQLTASFHSTHSSLLSRDAVT ALSFLIEGTVSRAKKVYPLHQLALWQPLHVASGFSKSSKTFSLYKLEAWLRASLTTNPFGPSACLKSGKK LAWAHQVEGSTKRAKIACNAHMAPRLHRIVVMSQVYKQTLAKSSETLVGAHVRAHRCSESFIYLLSPLRS MTIEKCRNSTFVLGPVETTLHLHDCENLKVIAVCHRLSISSTTSCTFHIMTPSRPLILSGNQTVTFAPFH THYPMLEDHMARTGLATVPNYWDNPMVVCKEGSVTSLFRLLPPSEFYIFVIPFEMEGDTAEIPGGLPSAY QKALAQREESIHIWQKTVKEARLTKEQRKQFQALVENKFYEWLVSTGHRQQLDSLVPTPAAS TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC082558
ORF Size	1658 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC082558, AAH82558
RefSeq Size	2693 bp
RefSeq ORF	1658 bp
Locus ID	70573
Gene Summary	Plays a role in the regulation of centrosome and Golgi apparatus positioning, with consequences on cell shape and cell migration.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.