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Mettl16 (NM_026197) Mouse Tagged ORF Clone
CAT＃: MG208777
- TrueORF®
Mettl16 (tGFP-tagged) - Mouse methyltransferase 10 domain containing (Mett10d)
ORF Plasmid: DDK
"NM_026197" in other vectors (4)
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CNY 5,040.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 2610100D03Rik; 2810013M15Rik; A830095F14Rik; AI846133; Mett10d |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG208777 representing NM_026197
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCTAAGCAAATCAATGCATGCAAGAAATAGATACAAGGACAAACCACCTGACTTCGCATATCTGG CATCTAAATATCCAGATTTTAAGCAGCATATTCAGATAAATCTGAATGGGAGAGTAAGTCTTAATTTTAA AGACCCCGAAGCAGTCAGAGCTCTGACTTGTACTCTCCTGAGAGAGGACTTTGGACTTTCTATTGATATT CCTTTGGAGAGACTAATTCCCACAGTCCCCTTGAGACTTAACTATATTCACTGGGTAGAAGATTTGATTG GTCACCAGGATTCGGACAAAACTACTCTCCGAAGAGGAATTGACATAGGTACTGGGGCATCCTGCATCTA TCCCTTACTTGGAGCAACCTTAAATGGCTGGTATTTCCTGGCAACAGAAGTGGATGATATGTGTTTCAAT TATGCCAAGAAAAATGTAGAGCAGAATAACCTATCTGATCTCATTAAAGTGGTGAAAGTGCCGCAGAAGA CACTCCTGATGGATGCGCTTAAGGAAGAGTCTGAGATAGTCTATGACTTCTGTATGTGCAACCCTCCCTT TTTTGCCAACCAATTGGAAGCCAAGGGTGTAAATTCCCGGAATTCTCGAAGACCTCCACCTAGTTCTGTA AACACCGGGGGCATCACAGAGATTATGGCAGAAGGAGGTGAGCTAGAATTTGTCAAAAGGATCATCCATG ACAGTTTACAGCTCAAAAAAAGGTTACGGTGGTATAGCTGTATGCTGGGAAAGAAATGCAGTCTGGCTCC ACTGAAGGAAGAGCTTAGAATCCAAGGGGTTCCTAAAGTCACCTTCACAGAGTTTTGTCAGGGTCGGACA ATGAGATGGGCCTTAGCTTGGAGTTTTTATGATGATGTCACAGTACCGTCACCTCCAAGTAAACGAAGAA AATTAGAAAAGCCAAGGAAACCCATTACTTTTGTAGTGTTGGAGTCTGTGATGAAAGAATTGTCTCTCAA AGCTTCATCTTTGGGCTCCGAGACAGCGGAAGGCATAGTGGTTGTAACAACATGGATAGAAAAGATCCTG ACTGATCTGAAGGTGCAACATAAGCGAATTCCCTGTGGAAGAGAAGAAGTTAGCCTTTTCCTGACAGCCA TAGAAAACTCTTGGATTCATTTAAGGCGGAAGAGAAGAGAGCGAGTGAGGCAGCTGCGGGAGGTGCCTCG GGCTCCTGAGGACGTCATCTTAGCCTTGGAAGAGAGAAAGTCCACTCCCAAAGAGCTGAGTAGTGGCCAA GACGTGGCCCACAGCCCCCAAGAGAGTGCCCTGTGTGGGCTTGATGTGCCGGGCGGCGAGGCTGCTGCTG ATGGAGGCCATTGCCTCAGCCAGAAGTTACTGTGCCAGGAGGAGACTCCCGAGGCCACGGAGGATGAAAG GGATGAGGAAAGGGGAGGGATGGAGGCCATGGAAAGTTGTAAAGGCTCCAGCAATGGAGCCCAGGACGGA GAGGCTTCTGAGAAGGGGGATCGCCTGGATGGGGCGGCCGGACGCTACCTGTTTAAGTGTTTAGTCAACA TTAAGAAGGAAGCTGGCGATGCCGTGGTTGAAATGCATTGGGTTGAGGGCCAGAATAGGGATTTGATGAA CCAGCTTTGCACTTACGTACGTAACCAAATTTTGAGGCTTGTTGCTAGT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG208777 representing NM_026197
Red=Cloning site Green=Tags(s) MALSKSMHARNRYKDKPPDFAYLASKYPDFKQHIQINLNGRVSLNFKDPEAVRALTCTLLREDFGLSIDI PLERLIPTVPLRLNYIHWVEDLIGHQDSDKTTLRRGIDIGTGASCIYPLLGATLNGWYFLATEVDDMCFN YAKKNVEQNNLSDLIKVVKVPQKTLLMDALKEESEIVYDFCMCNPPFFANQLEAKGVNSRNSRRPPPSSV NTGGITEIMAEGGELEFVKRIIHDSLQLKKRLRWYSCMLGKKCSLAPLKEELRIQGVPKVTFTEFCQGRT MRWALAWSFYDDVTVPSPPSKRRKLEKPRKPITFVVLESVMKELSLKASSLGSETAEGIVVVTTWIEKIL TDLKVQHKRIPCGREEVSLFLTAIENSWIHLRRKRRERVRQLREVPRAPEDVILALEERKSTPKELSSGQ DVAHSPQESALCGLDVPGGEAAADGGHCLSQKLLCQEETPEATEDERDEERGGMEAMESCKGSSNGAQDG EASEKGDRLDGAAGRYLFKCLVNIKKEAGDAVVEMHWVEGQNRDLMNQLCTYVRNQILRLVAS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_026197 |
| ORF Size | 1659 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_026197.3 |
| RefSeq Size | 2720 bp |
| RefSeq ORF | 1662 bp |
| Locus ID | 67493 |
| UniProt ID | Q9CQG2 |
| Gene Summary | RNA N6-methyltransferase that methylates adenosine residues at the N(6) position of a subset of RNAs and is involved in S-adenosyl-L-methionine homeostasis by regulating expression of MAT2A transcripts (PubMed:29262316, PubMed:30197299). Able to N6-methylate a subset of mRNAs and U6 small nuclear RNAs (U6 snRNAs) (By similarity). In contrast to the METTL3-METTL14 heterodimer, only able to methylate a limited number of RNAs: requires both a 5'UACAGAGAA-3' nonamer sequence and a specific RNA structure (By similarity). Plays a key role in S-adenosyl-L-methionine homeostasis by mediating N6-methylation of MAT2A mRNAs, altering splicing and/or stability of MAT2A transcripts: in presence of S-adenosyl-L-methionine, binds the 3' UTR region of MAT2A mRNA and specifically N6-methylates the first hairpin of MAT2A mRNA, impairing MAT2A expression (PubMed:29262316, PubMed:30197299). In S-adenosyl-L-methionine-limiting conditions, binds the 3' UTR region of MAT2A mRNA but stalls due to the lack of a methyl donor, preventing N6-methylation and promoting expression of MAT2A (PubMed:29262316). In addition to mRNAs, also able to mediate N6-methylation of U6 small nuclear RNA (U6 snRNA): specifically N6-methylates adenine in position 43 of U6 snRNAs (By similarity). Also able to bind various lncRNAs (By similarity). Specifically binds the 3'-end of the MALAT1 long non-coding RNA (By similarity) (PubMed:29262316, PubMed:30197299).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC203406 | Mettl16 (untagged) - Mouse methyltransferase like 16 (Mettl16), (10ug) |
CNY 4,128.00 |
|
| MR208777 | Mettl16 (Myc-DDK-tagged) - Mouse methyltransferase like 16 (Mettl16) |
CNY 4,128.00 |
|
| MR208777L3 | Lenti ORF clone of Mettl16 (Myc-DDK-tagged) - Mouse methyltransferase like 16 (Mettl16) |
CNY 6,460.00 |
|
| MR208777L4 | Lenti ORF clone of Mettl16 (mGFP-tagged) - Mouse methyltransferase like 16 (Mettl16) |
CNY 6,460.00 |
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