Cdc14a (BC072644) Mouse Tagged ORF Clone

CAT#: MG208789

  • TrueORF®

Cdc14a (tGFP-tagged) - Mouse CDC14 cell division cycle 14 homolog A (S



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Mouse Protein / Lysate Request

CNY 5,230.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


CDC14A Antibody - middle region
    • 100 ul

CNY 5,250.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms Cdc14, CDC14A2, CDC14a1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG208789 representing BC072644
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGCGGAGTCTGGGGAACTAATCGGGGCTTGCGAGTTCATGAAAGATCGATTATATTTTGCTACTT
TAAGGAATAGACCAAAAAGCACAATAAATATCCACTATTTCTCCATCGACGAGGAGCTGGTCTATGAAAA
TTTCTATGCAGATTTTGGACCTCTGAACTTGGCAATGGTGTACAGATACTGCTGTAAGCTAAACAAGAAA
CTAAAATCATACAGTTTATCAAGAAAGAAGATAGTGCACTACACCTCTTTCGACCAGCGGAAAAGAGCAA
ACGCAGCATTCTTGATAGGTGCTTATGCCGGATTACAGCATGGGTTTTTTGACTTTGAGACGTTTGATGC
GGAAGAATATGAACATTATGAGCGAGTTGAAAACGGAGACTTCAACTGGATCGTTCCAGGAAAGTTTTTA
GCGTTCAGTGGACCGCATCCTAAAAGCAAGATTGAGAATGGTTACCCTCTCCACGCTCCCGAAGCCTACT
TTCCATACTTCAAAAAGAACAACGTGACCACGATCGTGAGATTGAACAAAAAGATCTACGAGGCGAAGCG
CTTCACAGACGCTGGCTTCGAGCACTATGACCTGTTCTTCATAGACGGCAGCACCCCCAGCGATAACATC
GTGCGGAGATTCCTGAACATCTGTGAGAACACCGAGGGGGCCATCGCGGTCCACTGCAAAGCTGGTCTGG
GAAGAACCGGGACATTGATAGCCTGTTATGTCATGAAACACTACAGGTTTACACATGCTGAAATCATTGC
TTGGATCAGAATTTGCCGACCAGGCTCCATCATCGGACCCCAGCAGCACTTCCTGAAAGAAAAACAAGCA
TCATTGTGGGTCCAAGGAGACATTTTTAGATCCAAACTAAAAAATAGACCATCCAGTGAGGGAAGTATTA
CAAAAATTATTTCTACCCTGGATGATATGTCTATTGGTGCAAATTTATCCAAATTACAAAGCACAGAAAG
GATTGGAGAGAATAATTTTGAAGACGAAGATATGGAAATTAAGAACAACGTAACGCAGGGAGACAAACTA
CGTGCCTTAAAAAGCCAGAGGCATCCCCGCTCCTCGCCATCCTGTGCATTTAGGTCAGATGATATGAAAG
GACACCAAAGGGCAATGGCCCAGACTTTCAGATTAAGTTCCTCACCGCAACCAACTATGTCGACCATGAA
GACCTCCAAAGTGTGTTTGTCCCCTTCAGTGACAGCCAAAAAAATAAGCCGAGGTTCTTTGTCTTCAGGA
GCAAATATAAGAAGCTTCTCCATAAATTCCCGGCTAGCCAGTTCTCTAGGGAACTTGAACGCCGGGACAG
AAGAACCTGAGACCAAGAAGACCACATCACTCACCAAGGCCGCTTTCATAGCCAGCCCATTCACCAGCTT
CTTGAATGGCAGCACCCAGACACCTGGCAGAAACTACCCTGAGCTCAACAACAACCAGTACACCAGAAGC
AGCAACAGCAACAGCAGCAGCAGCAGCAGCGGTCTTGGGGGCAACCTGAACAGCTCTCCAGTCCCCCAGA
GCGCCAAGCCCGAGGAGCACACCACCATCCTTCGACCTTCCTTCCCGGGGTCACTCTCTTCCTCCTCAGT
GAGATTCCTGAGCCGTTCTATACCTTCCCTTCAGTCTGAATACGTTCATTAC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG208789 representing BC072644
Red=Cloning site Green=Tags(s)

MAAESGELIGACEFMKDRLYFATLRNRPKSTINIHYFSIDEELVYENFYADFGPLNLAMVYRYCCKLNKK
LKSYSLSRKKIVHYTSFDQRKRANAAFLIGAYAGLQHGFFDFETFDAEEYEHYERVENGDFNWIVPGKFL
AFSGPHPKSKIENGYPLHAPEAYFPYFKKNNVTTIVRLNKKIYEAKRFTDAGFEHYDLFFIDGSTPSDNI
VRRFLNICENTEGAIAVHCKAGLGRTGTLIACYVMKHYRFTHAEIIAWIRICRPGSIIGPQQHFLKEKQA
SLWVQGDIFRSKLKNRPSSEGSITKIISTLDDMSIGANLSKLQSTERIGENNFEDEDMEIKNNVTQGDKL
RALKSQRHPRSSPSCAFRSDDMKGHQRAMAQTFRLSSSPQPTMSTMKTSKVCLSPSVTAKKISRGSLSSG
ANIRSFSINSRLASSLGNLNAGTEEPETKKTTSLTKAAFIASPFTSFLNGSTQTPGRNYPELNNNQYTRS
SNSNSSSSSSGLGGNLNSSPVPQSAKPEEHTTILRPSFPGSLSSSSVRFLSRSIPSLQSEYVHY

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC072644
ORF Size 1664 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC072644, AAH72644
RefSeq Size 4319 bp
RefSeq ORF 1664 bp
Locus ID 229776
Gene Summary Dual-specificity phosphatase. Required for centrosome separation and productive cytokinesis during cell division. Dephosphorylates SIRT2 around early anaphase. May dephosphorylate the APC subunit FZR1/CDH1, thereby promoting APC-FZR1 dependent degradation of mitotic cyclins and subsequent exit from mitosis (By similarity). Required for normal hearing (PubMed:29293958).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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