Cdc14a (BC072644) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	Cdc14, CDC14A2, CDC14a1
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG208789 representing BC072644 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGCGGAGTCTGGGGAACTAATCGGGGCTTGCGAGTTCATGAAAGATCGATTATATTTTGCTACTT TAAGGAATAGACCAAAAAGCACAATAAATATCCACTATTTCTCCATCGACGAGGAGCTGGTCTATGAAAA TTTCTATGCAGATTTTGGACCTCTGAACTTGGCAATGGTGTACAGATACTGCTGTAAGCTAAACAAGAAA CTAAAATCATACAGTTTATCAAGAAAGAAGATAGTGCACTACACCTCTTTCGACCAGCGGAAAAGAGCAA ACGCAGCATTCTTGATAGGTGCTTATGCCGGATTACAGCATGGGTTTTTTGACTTTGAGACGTTTGATGC GGAAGAATATGAACATTATGAGCGAGTTGAAAACGGAGACTTCAACTGGATCGTTCCAGGAAAGTTTTTA GCGTTCAGTGGACCGCATCCTAAAAGCAAGATTGAGAATGGTTACCCTCTCCACGCTCCCGAAGCCTACT TTCCATACTTCAAAAAGAACAACGTGACCACGATCGTGAGATTGAACAAAAAGATCTACGAGGCGAAGCG CTTCACAGACGCTGGCTTCGAGCACTATGACCTGTTCTTCATAGACGGCAGCACCCCCAGCGATAACATC GTGCGGAGATTCCTGAACATCTGTGAGAACACCGAGGGGGCCATCGCGGTCCACTGCAAAGCTGGTCTGG GAAGAACCGGGACATTGATAGCCTGTTATGTCATGAAACACTACAGGTTTACACATGCTGAAATCATTGC TTGGATCAGAATTTGCCGACCAGGCTCCATCATCGGACCCCAGCAGCACTTCCTGAAAGAAAAACAAGCA TCATTGTGGGTCCAAGGAGACATTTTTAGATCCAAACTAAAAAATAGACCATCCAGTGAGGGAAGTATTA CAAAAATTATTTCTACCCTGGATGATATGTCTATTGGTGCAAATTTATCCAAATTACAAAGCACAGAAAG GATTGGAGAGAATAATTTTGAAGACGAAGATATGGAAATTAAGAACAACGTAACGCAGGGAGACAAACTA CGTGCCTTAAAAAGCCAGAGGCATCCCCGCTCCTCGCCATCCTGTGCATTTAGGTCAGATGATATGAAAG GACACCAAAGGGCAATGGCCCAGACTTTCAGATTAAGTTCCTCACCGCAACCAACTATGTCGACCATGAA GACCTCCAAAGTGTGTTTGTCCCCTTCAGTGACAGCCAAAAAAATAAGCCGAGGTTCTTTGTCTTCAGGA GCAAATATAAGAAGCTTCTCCATAAATTCCCGGCTAGCCAGTTCTCTAGGGAACTTGAACGCCGGGACAG AAGAACCTGAGACCAAGAAGACCACATCACTCACCAAGGCCGCTTTCATAGCCAGCCCATTCACCAGCTT CTTGAATGGCAGCACCCAGACACCTGGCAGAAACTACCCTGAGCTCAACAACAACCAGTACACCAGAAGC AGCAACAGCAACAGCAGCAGCAGCAGCAGCGGTCTTGGGGGCAACCTGAACAGCTCTCCAGTCCCCCAGA GCGCCAAGCCCGAGGAGCACACCACCATCCTTCGACCTTCCTTCCCGGGGTCACTCTCTTCCTCCTCAGT GAGATTCCTGAGCCGTTCTATACCTTCCCTTCAGTCTGAATACGTTCATTAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG208789 representing BC072644 Red=Cloning site Green=Tags(s) MAAESGELIGACEFMKDRLYFATLRNRPKSTINIHYFSIDEELVYENFYADFGPLNLAMVYRYCCKLNKK LKSYSLSRKKIVHYTSFDQRKRANAAFLIGAYAGLQHGFFDFETFDAEEYEHYERVENGDFNWIVPGKFL AFSGPHPKSKIENGYPLHAPEAYFPYFKKNNVTTIVRLNKKIYEAKRFTDAGFEHYDLFFIDGSTPSDNI VRRFLNICENTEGAIAVHCKAGLGRTGTLIACYVMKHYRFTHAEIIAWIRICRPGSIIGPQQHFLKEKQA SLWVQGDIFRSKLKNRPSSEGSITKIISTLDDMSIGANLSKLQSTERIGENNFEDEDMEIKNNVTQGDKL RALKSQRHPRSSPSCAFRSDDMKGHQRAMAQTFRLSSSPQPTMSTMKTSKVCLSPSVTAKKISRGSLSSG ANIRSFSINSRLASSLGNLNAGTEEPETKKTTSLTKAAFIASPFTSFLNGSTQTPGRNYPELNNNQYTRS SNSNSSSSSSGLGGNLNSSPVPQSAKPEEHTTILRPSFPGSLSSSSVRFLSRSIPSLQSEYVHY TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC072644
ORF Size	1662 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC072644, AAH72644
RefSeq Size	4319 bp
RefSeq ORF	1664 bp
Locus ID	229776
Gene Summary	Dual-specificity phosphatase. Required for centrosome separation and productive cytokinesis during cell division. Dephosphorylates SIRT2 around early anaphase. May dephosphorylate the APC subunit FZR1/CDH1, thereby promoting APC-FZR1 dependent degradation of mitotic cyclins and subsequent exit from mitosis (By similarity). Required for normal hearing (PubMed:29293958).[UniProtKB/Swiss-Prot Function]

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