United States
Germany
Japan
United Kingdom
China
Wasf1 (NM_031877) Mouse Tagged ORF Clone
CAT＃: MG208865
- TrueORF®
Wasf1 (tGFP-tagged) - Mouse WASP family 1 (Wasf1)
ORF Plasmid: DDK
"NM_031877" in other vectors (3)
Need custom modification / cloning service?
Get a free quote
CNY 5,040.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AI195380; AI838537; Scar; WAVE; WAVE-1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG208865 representing NM_031877
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGTTGGTGAAAAGAAACATCGACCCTCGGCACTTGTGCCACACAGCACTGCCCAGGGGCATTAAGA ACGAACTGGAATGTGTAACCAACATTTCCCTGGCAAATATAATTAGACAACTGAGTAGCCTAAGTAAGTA TGCTGAAGACATATTTGGAGAATTATTCAATGAAGCACACAGTTTTTCCTTCAGAGTTAACTCGTTGCAA GAGCGCGTGGACCGATTGTCTGTTAGTGTCACACAGCTTGATCCTAAGGAGGAAGAACTGTCTTTGCAAG ATATAACGATGAGAAAGGCTTTCCGGAGCTCCACAATTCAAGATCAGCAGCTTTTCGACCGCAAGACGTT GCCTATTCCATTGCAAGAAACATACGATGTGTGTGAGCAACCTCCGCCTCTCAATATCCTCACCCCTTAC AGGGATGATGGTAAGGAAGGGCTGAAGTTTTATACCAATCCTTCATATTTCTTTGATCTGTGGAAAGAAA AGATGTTGCAAGATACAGAGGACAAGAGGAAGGAAAAGAGAAAGCAGAAGCAGAAAAATCTAGACCGTCC TCATGAACCAGAGAAAGTGCCAAGAGCACCTCATGACAGGCGGCGGGAATGGCAGAAGCTGGCCCAAGGT CCAGAGCTGGCTGAGGATGACGCTGACCTCCTACACAAGCATATTGAAGTTGCCAATGGCCCAGCCTCTC ATTATGAGACAAGGCCACAGACATACGTGGATCATATGGACGGATCGTACTCACTCTCTGCCTTGCCATT CAGTCAGATGAGTGAACTTCTGACCAGAGCTGAGGAGAGGGTCTTAGTCAGACCCCATGAACCTCCTCCG CCTCCACCAATGCATGGAGCAGGAGATGCAAAGCCCACACCCACCTGTATCAGTTCTGCTACAGGTTTGA TAGAGAATCGCCCCCAGTCCCCAGCTGCAGGCAGAACACCTGTGTTTGTGAGCCCCACTTCCCCACCTCC TCCCCCACCTCTTCCGTCTGCCTTGTCCACTTCTTCACTAAGAGCTTCAATGACCTCAACTCCTCCCCCA CCAGTCCCTCCTCCGCCTCCTCCCCCAGCCACTGCTTTGCAGGCCCCAGCAGTGCCCCCACCTCCGGCTC CTCTTCAGATTGCCCCTGGTGTTCTTCACCCAGCTCCTCCTCCAATTGCTCCTCCTCTAGTGCAGCCCTC TCCCCCAGTAGCTCGAGCTGCCCCAGTATGCGAGACTGTGCCAGTTCACCCACTCCCGCAAGGTGAAGTG CAGGGGCTGCCTCCACCCCCACCACCGCCTCCTCTACCTCCGCCTGGCATCCGGCCATCATCTCCTGTCG CAGTTGCAGCTCTTGCTCATCCTCCCTCTGGGCTCCATCCAGCACCATCTACTGCCCCGGGTCCCCATGC TCCATTAATGCCTCCATCTCCTCCATCACAAGTTCTACCTGCCTCTGAGCCAAAGCGCCATCCATCCACC CTGCCTGTAATCAGTGACGCAAGGAGTGTGCTGCTGGAAGCCATCCGAAAAGGTATTCAGCTTCGCAAAG TGGAAGAGCAGCGTGAACAGGAAGCAAAACACGAGCGGATTGAAAACGACGTGGCCACCATCCTGTCTCG CCGCATTGCTGTGGAGTATAGCGACTCGGAAGATGACTCGGAGTTCGATGAAGTAGACTGGCTGGAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG208865 representing NM_031877
Red=Cloning site Green=Tags(s) MPLVKRNIDPRHLCHTALPRGIKNELECVTNISLANIIRQLSSLSKYAEDIFGELFNEAHSFSFRVNSLQ ERVDRLSVSVTQLDPKEEELSLQDITMRKAFRSSTIQDQQLFDRKTLPIPLQETYDVCEQPPPLNILTPY RDDGKEGLKFYTNPSYFFDLWKEKMLQDTEDKRKEKRKQKQKNLDRPHEPEKVPRAPHDRRREWQKLAQG PELAEDDADLLHKHIEVANGPASHYETRPQTYVDHMDGSYSLSALPFSQMSELLTRAEERVLVRPHEPPP PPPMHGAGDAKPTPTCISSATGLIENRPQSPAAGRTPVFVSPTSPPPPPPLPSALSTSSLRASMTSTPPP PVPPPPPPPATALQAPAVPPPPAPLQIAPGVLHPAPPPIAPPLVQPSPPVARAAPVCETVPVHPLPQGEV QGLPPPPPPPPLPPPGIRPSSPVAVAALAHPPSGLHPAPSTAPGPHAPLMPPSPPSQVLPASEPKRHPST LPVISDARSVLLEAIRKGIQLRKVEEQREQEAKHERIENDVATILSRRIAVEYSDSEDDSEFDEVDWLE TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_031877 |
| ORF Size | 1677 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_031877.2, NP_114083.1 |
| RefSeq Size | 2574 bp |
| RefSeq ORF | 1680 bp |
| Locus ID | 83767 |
| UniProt ID | Q8R5H6 |
| Gene Summary | Downstream effector molecule involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Promotes formation of actin filaments. Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex (By similarity). As component of the WAVE1 complex, required for BDNF-NTRK2 endocytic trafficking and signaling from early endosomes (PubMed:27605705).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR208865 | Wasf1 (Myc-DDK-tagged) - Mouse WASP family 1 (Wasf1) |
CNY 4,168.00 |
|
| MR208865L3 | Lenti ORF clone of Wasf1 (Myc-DDK-tagged) - Mouse WASP family 1 (Wasf1) |
CNY 6,460.00 |
|
| MR208865L4 | Lenti ORF clone of Wasf1 (mGFP-tagged) - Mouse WASP family 1 (Wasf1) |
CNY 6,460.00 |
