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Xrn2 (BC004028) Mouse Tagged ORF Clone
CAT＃: MG208891
- TrueORF®
Xrn2 (tGFP-tagged) - Mouse 5'-3' exoribonuclease 2 (cDNA clone MGC:7624 IMAGE:3495063)
ORF Plasmid: DDK
"BC004028" in other vectors (3)
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CNY 6,180.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG208891 representing BC004028
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATCATGTTAGCAGTTGGTGAAGTTGAGGATAGCATTTTTAAAAAGAGAAAAGATGATGAGGACAGTT TTAGAAGACGACAGAAAGAAAAAAGAAAGAGGATGAAGAGAGATCAACCAGCTTTTACTCCTAGTGGAAT ATTAACACCTCATGCCTTGGGTTCAAGAAATTCACCAGGTTGTCAAGTAGCCAGTAATCCAAGACAAGCA GCCTATGAAATGAGGATGCAGAGAAACTCTAGTCCTTCAATATCTCCTAATACAAGTTTTGCATCTGATG GCTCCCCATCTCCACTAGGAGGAATTAAGAGAAAAGCAGAAGACAGTGACAGTGAGCCAGAGCCAGAGGA TAACGTCAGGTTATGGGAAGCTGGTTGGAAGCAACGATACTACAAGAACAAATTTGATGTAGATGCAGCT GATGAGAAATTCCGACGTAAGGTTGTTCAGTCCTACGTTGAAGGACTGTGCTGGGTTCTTCGCTATTATT ACCAGGGCTGTGCTTCCTGGAAGTGGTATTATCCATTCCATTATGCACCATTTGCCTCAGACTTTGAAGG TATTGCAGACATGTCCTCTGAATTTGAAAAGGGCACAAAACCGTTTAAGCCACTGGAACAACTAATGGGG GTTTTCCCAGCTGCAAGTGGTAACTTTCTACCTCCAACATGGCGGAAGCTCATGAGTGACCCTGATTCCA GTATAATTGACTTCTATCCTGAAGATTTTGCTATTGATTTGAATGGGAAGAAATATGCATGGCAAGGTGT TGCTCTATTGCCATTTGTGGATGAGCGAAGGCTGCGAGCGGCTCTAGAAGAGGTGTACCCAGACCTCACT CCAGAAGAGAACAGGAGAAATAGTCTTGGAGGTGATGTTTTGTTTGTGGGAAAACTTCATCCACTACGCG ACTTCATTTTAGAGCTGTATCAGACGGGTTCCACAGAGCCAGTTGATGTGCCACCTGAATTGTGTCACGG GATTCAAGGGACGTTTTCTTTGGATGAAGAAGCCATTCTTCCAGACCAAACAGTATGTTCCCCTGTGCCC ATGTTGCGGGACCTGACACAGAACACTGCAGTCAGTATTAATTTTAAAGATCCACAGTTTGCTGAAGATT ATGTTTTTAAAGCTGCAATGCTTCCAGGAGCAAGAAAGCCAGCAACAGTTCTGAAACCTGGTGACTGGGA AAAATCCAGCAATGGGCGGCAATGGAAACCTCAGCTTGGCTTTAACCGGGACCGGCGGCCTGTTCACCTG GACCAGGCAGCCTTTAGAACTTTAGGCCATGTTACACCAAGAGGCTCGGGGACGAGTGTTTATACGAACA CTGCACTACCACCTGCCAATTACCAGGGGAACAATTACAGGCCACTGCTGAGAGGTCAAGCTCAGATCCC AAAACTTATGTCAAATATGAGGCCCCAAGATTCCTGGCGAGGCCCTCCTCCTCTTTTCCAGCAGCATAGA TTTGAGAGAAGTGTTGGAGCTGAACCTCTACTGCCATGGAACCGGATGATCCAAAACCAAAATGCAGCCT TTCAGCCAAATCAGTACCAGATGCTAGGAGGACCTGGAGGCTATCCACCCAGACGTGACGATCACCGAGG AGGGAGACAGGGATATCCCAGAGAAGGACGGAAATACCCTTTGCCACCACCCTCGGGAAGATACAGTTGG AAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG208891 representing BC004028
Red=Cloning site Green=Tags(s) MIMLAVGEVEDSIFKKRKDDEDSFRRRQKEKRKRMKRDQPAFTPSGILTPHALGSRNSPGCQVASNPRQA AYEMRMQRNSSPSISPNTSFASDGSPSPLGGIKRKAEDSDSEPEPEDNVRLWEAGWKQRYYKNKFDVDAA DEKFRRKVVQSYVEGLCWVLRYYYQGCASWKWYYPFHYAPFASDFEGIADMSSEFEKGTKPFKPLEQLMG VFPAASGNFLPPTWRKLMSDPDSSIIDFYPEDFAIDLNGKKYAWQGVALLPFVDERRLRAALEEVYPDLT PEENRRNSLGGDVLFVGKLHPLRDFILELYQTGSTEPVDVPPELCHGIQGTFSLDEEAILPDQTVCSPVP MLRDLTQNTAVSINFKDPQFAEDYVFKAAMLPGARKPATVLKPGDWEKSSNGRQWKPQLGFNRDRRPVHL DQAAFRTLGHVTPRGSGTSVYTNTALPPANYQGNNYRPLLRGQAQIPKLMSNMRPQDSWRGPPPLFQQHR FERSVGAEPLLPWNRMIQNQNAAFQPNQYQMLGGPGGYPPRRDDHRGGRQGYPREGRKYPLPPPSGRYSW N TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC004028 |
| ORF Size | 1686 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC004028.1 |
| RefSeq Size | 2552 bp |
| RefSeq ORF | 1685 bp |
| Locus ID | 24128 |
| Gene Summary | Possesses 5'->3' exoribonuclease activity. May promote the termination of transcription by RNA polymerase II. During transcription termination, cleavage at the polyadenylation site liberates a 5' fragment which is subsequently processed to form the mature mRNA and a 3' fragment which remains attached to the elongating polymerase. The processive degradation of this 3' fragment by this protein may promote termination of transcription. Binds to RNA polymerase II (RNAp II) transcription termination R-loops formed by G-rich pause sites (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR208891 | Xrn2 (Myc-DDK-tagged) - Mouse 5'-3' exoribonuclease 2 (cDNA clone MGC:7624 IMAGE:3495063) |
CNY 5,610.00 |
|
| MR208891L3 | Lenti ORF clone of Xrn2 (Myc-DDK-tagged) - Mouse 5'-3' exoribonuclease 2 (cDNA clone MGC:7624 IMAGE:3495063) |
CNY 7,510.00 |
|
| MR208891L4 | Lenti ORF clone of Xrn2 (mGFP-tagged) - Mouse 5'-3' exoribonuclease 2 (cDNA clone MGC:7624 IMAGE:3495063) |
CNY 7,510.00 |
