Xrn2 (BC004028) Mouse Tagged ORF Clone

CAT#: MG208891

  • TrueORF®

Xrn2 (tGFP-tagged) - Mouse 5'-3' exoribonuclease 2 (cDNA clone MGC:7624 IMAGE:3495063)

ORF Plasmid: DDK tGFP



  "BC004028" in other vectors (3)

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Mouse Protein / Lysate Request

CNY 6,180.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


XRN2 Antibody - C-terminal region
    • 50 ug

CNY 4,628.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG208891 representing BC004028
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATCATGTTAGCAGTTGGTGAAGTTGAGGATAGCATTTTTAAAAAGAGAAAAGATGATGAGGACAGTT
TTAGAAGACGACAGAAAGAAAAAAGAAAGAGGATGAAGAGAGATCAACCAGCTTTTACTCCTAGTGGAAT
ATTAACACCTCATGCCTTGGGTTCAAGAAATTCACCAGGTTGTCAAGTAGCCAGTAATCCAAGACAAGCA
GCCTATGAAATGAGGATGCAGAGAAACTCTAGTCCTTCAATATCTCCTAATACAAGTTTTGCATCTGATG
GCTCCCCATCTCCACTAGGAGGAATTAAGAGAAAAGCAGAAGACAGTGACAGTGAGCCAGAGCCAGAGGA
TAACGTCAGGTTATGGGAAGCTGGTTGGAAGCAACGATACTACAAGAACAAATTTGATGTAGATGCAGCT
GATGAGAAATTCCGACGTAAGGTTGTTCAGTCCTACGTTGAAGGACTGTGCTGGGTTCTTCGCTATTATT
ACCAGGGCTGTGCTTCCTGGAAGTGGTATTATCCATTCCATTATGCACCATTTGCCTCAGACTTTGAAGG
TATTGCAGACATGTCCTCTGAATTTGAAAAGGGCACAAAACCGTTTAAGCCACTGGAACAACTAATGGGG
GTTTTCCCAGCTGCAAGTGGTAACTTTCTACCTCCAACATGGCGGAAGCTCATGAGTGACCCTGATTCCA
GTATAATTGACTTCTATCCTGAAGATTTTGCTATTGATTTGAATGGGAAGAAATATGCATGGCAAGGTGT
TGCTCTATTGCCATTTGTGGATGAGCGAAGGCTGCGAGCGGCTCTAGAAGAGGTGTACCCAGACCTCACT
CCAGAAGAGAACAGGAGAAATAGTCTTGGAGGTGATGTTTTGTTTGTGGGAAAACTTCATCCACTACGCG
ACTTCATTTTAGAGCTGTATCAGACGGGTTCCACAGAGCCAGTTGATGTGCCACCTGAATTGTGTCACGG
GATTCAAGGGACGTTTTCTTTGGATGAAGAAGCCATTCTTCCAGACCAAACAGTATGTTCCCCTGTGCCC
ATGTTGCGGGACCTGACACAGAACACTGCAGTCAGTATTAATTTTAAAGATCCACAGTTTGCTGAAGATT
ATGTTTTTAAAGCTGCAATGCTTCCAGGAGCAAGAAAGCCAGCAACAGTTCTGAAACCTGGTGACTGGGA
AAAATCCAGCAATGGGCGGCAATGGAAACCTCAGCTTGGCTTTAACCGGGACCGGCGGCCTGTTCACCTG
GACCAGGCAGCCTTTAGAACTTTAGGCCATGTTACACCAAGAGGCTCGGGGACGAGTGTTTATACGAACA
CTGCACTACCACCTGCCAATTACCAGGGGAACAATTACAGGCCACTGCTGAGAGGTCAAGCTCAGATCCC
AAAACTTATGTCAAATATGAGGCCCCAAGATTCCTGGCGAGGCCCTCCTCCTCTTTTCCAGCAGCATAGA
TTTGAGAGAAGTGTTGGAGCTGAACCTCTACTGCCATGGAACCGGATGATCCAAAACCAAAATGCAGCCT
TTCAGCCAAATCAGTACCAGATGCTAGGAGGACCTGGAGGCTATCCACCCAGACGTGACGATCACCGAGG
AGGGAGACAGGGATATCCCAGAGAAGGACGGAAATACCCTTTGCCACCACCCTCGGGAAGATACAGTTGG
AAT


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG208891 representing BC004028
Red=Cloning site Green=Tags(s)

MIMLAVGEVEDSIFKKRKDDEDSFRRRQKEKRKRMKRDQPAFTPSGILTPHALGSRNSPGCQVASNPRQA
AYEMRMQRNSSPSISPNTSFASDGSPSPLGGIKRKAEDSDSEPEPEDNVRLWEAGWKQRYYKNKFDVDAA
DEKFRRKVVQSYVEGLCWVLRYYYQGCASWKWYYPFHYAPFASDFEGIADMSSEFEKGTKPFKPLEQLMG
VFPAASGNFLPPTWRKLMSDPDSSIIDFYPEDFAIDLNGKKYAWQGVALLPFVDERRLRAALEEVYPDLT
PEENRRNSLGGDVLFVGKLHPLRDFILELYQTGSTEPVDVPPELCHGIQGTFSLDEEAILPDQTVCSPVP
MLRDLTQNTAVSINFKDPQFAEDYVFKAAMLPGARKPATVLKPGDWEKSSNGRQWKPQLGFNRDRRPVHL
DQAAFRTLGHVTPRGSGTSVYTNTALPPANYQGNNYRPLLRGQAQIPKLMSNMRPQDSWRGPPPLFQQHR
FERSVGAEPLLPWNRMIQNQNAAFQPNQYQMLGGPGGYPPRRDDHRGGRQGYPREGRKYPLPPPSGRYSW
N

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC004028
ORF Size 1686 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC004028.1
RefSeq Size 2552 bp
RefSeq ORF 1685 bp
Locus ID 24128
Gene Summary Possesses 5'->3' exoribonuclease activity. May promote the termination of transcription by RNA polymerase II. During transcription termination, cleavage at the polyadenylation site liberates a 5' fragment which is subsequently processed to form the mature mRNA and a 3' fragment which remains attached to the elongating polymerase. The processive degradation of this 3' fragment by this protein may promote termination of transcription. Binds to RNA polymerase II (RNAp II) transcription termination R-loops formed by G-rich pause sites (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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