Mtmr2 (BC063050) Mouse Tagged ORF Clone

CAT#: MG208990

  • TrueORF®

Mtmr2 (tGFP-tagged) - Mouse myotubularin related protein 2 (cDNA clone MGC:66658 IMAGE:5716297)

ORF Plasmid: DDK tGFP



  "BC063050" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 3,140.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms 6030445P13Rik
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG208990 representing BC063050
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGAACCAGCCCTGCTTCCAGGAGAGAACATTAAAGATATGGCCAAAGATGTGACTTACATATGTC
CATTCACTGGTGCTGTGCGAGGAACGCTGACTGTCACAAGTTACAGGTTATATTTCAAGAGCATGGAGCG
GGACCCCCCATTTGTTCTAGATGCTTCTCTTGGAGTGATAAGTAGAGTAGAAAAAATTGGTGGTGCATCT
AGCCGAGGTGAAAACTCCTATGGCCTGGAAACTGTGTGCAAGGATATCAGGAACTTGCGATTTGCTCATA
AACCGGAGGGGCGAACAAGAAGATCCATATTTGAGAATCTAATGAAATATGCATTTCCTGTGTCTAATGG
CTTGCCTCTTTTTGCTTTTGAGTATAAAGAAGTATTCCCCGAAAATGGGTGGAAACTGTATGACCCCCTT
CTAGAGTACAGAAGGCAGGGAATTCCAAATGAAAGCTGGAGAATAACAAAGATAAATGAACGCTATGAAC
TCTGTGACACATATCCTGCCCTCTTGGTTGTGCCAGCAAACATTCCTGATGAGGAGCTAAAGAGAGTGGC
ATCCTTTAGGTCACGGGGCCGGATCCCAGTTTTATCCTGGATTCATCCTGAAAGTCAAGCCACAGTCACT
CGCTGCAGCCAGCCCATGGTTGGAGTGAGTGGGAAGCGCAGCAAAGAAGATGAGAAGTACCTTCAGGCCA
TCATGGACTCCAATGCCCAGTCTCACAAAATATTTATCTTTGATGCTCGGCCAAGTGTCAATGCGGTTGC
CAATAAGGCAAAGGGGGGAGGTTATGAAAGTGAAGATGCCTATCAAAATGCAGAACTCGTTTTTCTGGAT
ATTCATAATATCCATGTTATGAGAGAATCATTACGGAAGCTTAAGGAGATTGTGTACCCCACCATTGAAG
AAACTCACTGGTTGTCTAACTTAGAATCTACACACTGGCTGGAACACATTAAGCTCATTCTTGCAGGGGC
TCTTAGGATTGCTGACAAGGTCGAGTCTGGGAAGACGTCTGTGGTGGTACACTGCAGTGATGGATGGGAT
CGCACAGCTCAGCTCACTTCCCTGGCCATGCTCATGTTGGATGGATACTACCGAACCATACGAGGATTTG
AAGTCCTTGTGGAGAAAGAATGGCTGAGCTTTGGACATAGATTCCAACTAAGAGTTGGCCATGGAGATAA
GAACCATGCTGACGCAGACAGGTCACCTGTTTTCCTTCAGTTTATTGACTGTGTCTGGCAAATGACAAGA
CAGTTCCCTACCGCATTTGAATTCAATGAGTATTTTCTCATTACCATCCTGGACCACTTGTACAGCTGTT
TATTTGGAACATTCCTTTGTAACAGCGAACAGCAGAGGGGGAAAGAGAATCTTCCTAAGAAGACAGTGTC
ACTGTGGTCCTATATAAACAGCCAGCTGGAAGACTTTACTAATCCTCTTTATGGAAGCTACTCCAATCAT
GTCCTTTACCCAGTAGCCAGCATGCGCCACCTAGAGCTCTGGGTGGGATATTATATAAGGTGGAACCCCC
GGATGAAACCTCAGGAACCTATCCACAGCAGATACAAAGAACTTCTTGCCAAAAGAGCAGAGCTTCAAAG
AAAAGTAGAGGAACTTCAGAGAGAAATTTCCAACCGCTCAACCTCCTCTTCAGAGAGAGCCAGCTCTCCT
GCACAGTGTGTGACTCCTGTCCAAACTGTTGTA


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG208990 representing BC063050
Red=Cloning site Green=Tags(s)

MEEPALLPGENIKDMAKDVTYICPFTGAVRGTLTVTSYRLYFKSMERDPPFVLDASLGVISRVEKIGGAS
SRGENSYGLETVCKDIRNLRFAHKPEGRTRRSIFENLMKYAFPVSNGLPLFAFEYKEVFPENGWKLYDPL
LEYRRQGIPNESWRITKINERYELCDTYPALLVVPANIPDEELKRVASFRSRGRIPVLSWIHPESQATVT
RCSQPMVGVSGKRSKEDEKYLQAIMDSNAQSHKIFIFDARPSVNAVANKAKGGGYESEDAYQNAELVFLD
IHNIHVMRESLRKLKEIVYPTIEETHWLSNLESTHWLEHIKLILAGALRIADKVESGKTSVVVHCSDGWD
RTAQLTSLAMLMLDGYYRTIRGFEVLVEKEWLSFGHRFQLRVGHGDKNHADADRSPVFLQFIDCVWQMTR
QFPTAFEFNEYFLITILDHLYSCLFGTFLCNSEQQRGKENLPKKTVSLWSYINSQLEDFTNPLYGSYSNH
VLYPVASMRHLELWVGYYIRWNPRMKPQEPIHSRYKELLAKRAELQRKVEELQREISNRSTSSSERASSP
AQCVTPVQTVV

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC063050
ORF Size 1713 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC063050, AAH63050
RefSeq Size 3125 bp
RefSeq ORF 1715 bp
Locus ID 77116
Gene Summary Phosphatase that acts on lipids with a phosphoinositol headgroup (PubMed:12045210, PubMed:16399794). Has phosphatase activity towards pho sphatidylinositol 3-phosphate and phosphatidylinositol 3,5-bisphosphate (PubMed:12045210, PubMed:16399794). Binds phosphatidylinositol 4-phosphate, phosphatidylinositol 5-phosphate, phosphatidylinositol 3,5-bisphosphate and phosphatidylinositol 3,4,5-trisphosphate (PubMed:12045210, PubMed:16399794). Stabilizes SBF2/MTMR13 at the membranes (PubMed:23297362). Specifically in peripheral nerves, stabilizes SBF2/MTMR13 protein (PubMed:23297362).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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