Scfd1 (BC027793) Mouse Tagged ORF Clone

CAT#: MG209036

  • TrueORF®

Scfd1 (tGFP-tagged) - Mouse sec1 family domain containing 1 (cDNA clone MGC:38021 IMAGE:5151118)

ORF Plasmid: DDK tGFP



  "BC027793" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 3,710.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms RA410, STXBP1L2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG209036 representing BC027793
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTGAATTTCAATGTGCCTCATGTTAAAAACAGTACTGGAGAACCAGTATGGAAGGTACTCATTTATG
ACAGATTTGGCCAAGATATCATCTCTCCTCTGCTGTCTGTGAAGGAACTGAGAGACATGGGCATCACTCT
GCATCTCCTTTTGCACTCAGACCGAGATCCGATTCCAGATGTTCCTGCAGTGTACTTTGTGATGCCAACC
GAAGAAAATATTGACAGACTGTGCCAGGATCTTCGAAATCAGCTCTATGAATCCTATTATTTAAATTTTA
TTTCTGCGATTTCAAGAAGTAAACTGGAAGACATTGCGAATGCAGCATTGGCCGCTAGTGCAGTCACACA
AGTTGCCAAGGTTTTTGACCAGTATCTCAATTTTATTACTTTGGAAGATGACATGTTTGTATTATGTAAT
CAAAATAAGGAACTTGTTTCATATCGTGCCATTAACAGGCCAGACATCACAGACACAGAGATGGAAACTG
TTATGGACACTATTGTGGACAGCCTCTTCTGCTTTTTTGTTACATTAGGTGCTGTTCCCATCATCCGATG
CTCAAGAGGAACGGCGGCAGAAATGGTGGCAGTGAAACTAGATAAAAAACTTCGGGAAAATCTAAGAGAT
GCAAGAAACAGCCTTTTCACCGGTGATCCACTTGGAACTGGCCAGTTCAGCTTCCAAAGGCCTTTATTAG
TCCTTGTTGACAGAAACATTGACCTGGCAACGCCTTTACACCATACGTGGACATACCAAGCGCTGGTACA
CGATGTGCTGGATTTCCACTTAAACAGAGTAAATTTGGAAGAATCTACAGGAGTGGAAAACTCTCCAGCT
GGTGCTAGACCAAAGAGGAAAAACAAGAAGTCTTACGACTTAACTCCAGTTGATAAATTCTGGCAGAAGC
ATAAAGGAAGTCCATTCCCAGAAGTCGCAGAATCAGTCCAACAAGAACTAGAATCTTACAGAGCACAAGA
AGATGAGGTCAAACGACTGAAGAGCATTATGGGACTAGAAGGAGAGGATGAAGGGGCCATCAGCATGCTT
TCTGATAACACTGCTAAGCTCACATCAGCTGTCAGTTCTTTGCCAGAACTCCTTGAAAAAAAAAGACTTA
TTGATCTGCACACAAATGTTGCCACTGCTGTTTTAGAACACATAAAGGCAAGAAAACTGGATGTATATTT
TGAATATGAAGAAAAAATAATGAGCAAGACTACTCTGGATAAGTCCCTCCTAGACGTCATATCCGACCCT
GATGCAGGGACTCCGGAAGACAAAATGAGGCTGTTTCTTATCTATTACATAAGCGCACAGCAAGCACCGT
CCGAGGTTGATTTGGAGCAGTATAAAAAGGCTTTAACAGATGCAGGCTGCAACCTTAGCCCTTTACAGTA
TATCAAGCAGTGGAAGGCTTTTGCCAAGATGGCCTCAACTCCTGCCAGCTACGGAAACACTACCACTAAA
CCAATGGGTCTCCTGTCCCGAGTCATGAATACAGGATCACAGTTTGTGATGGAAGGTGTGAAGAACCTGG
TATTGAAGCAGCAGAATCTACCTGTTACTCGGATTTTAGACAATCTCATGGAGATGAAGTCAAACCCTGA
AACTGATGATTATAGATATTTTGATCCCAAAATGCTGCGGAGTAATGACAGCTCCTTCCTCTACAAAACT
GAAGAACCCCCTGCTTCCCAGGAAGGTGGACCTGGAATACCTTCT


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG209036 representing BC027793
Red=Cloning site Green=Tags(s)

MLNFNVPHVKNSTGEPVWKVLIYDRFGQDIISPLLSVKELRDMGITLHLLLHSDRDPIPDVPAVYFVMPT
EENIDRLCQDLRNQLYESYYLNFISAISRSKLEDIANAALAASAVTQVAKVFDQYLNFITLEDDMFVLCN
QNKELVSYRAINRPDITDTEMETVMDTIVDSLFCFFVTLGAVPIIRCSRGTAAEMVAVKLDKKLRENLRD
ARNSLFTGDPLGTGQFSFQRPLLVLVDRNIDLATPLHHTWTYQALVHDVLDFHLNRVNLEESTGVENSPA
GARPKRKNKKSYDLTPVDKFWQKHKGSPFPEVAESVQQELESYRAQEDEVKRLKSIMGLEGEDEGAISML
SDNTAKLTSAVSSLPELLEKKRLIDLHTNVATAVLEHIKARKLDVYFEYEEKIMSKTTLDKSLLDVISDP
DAGTPEDKMRLFLIYYISAQQAPSEVDLEQYKKALTDAGCNLSPLQYIKQWKAFAKMASTPASYGNTTTK
PMGLLSRVMNTGSQFVMEGVKNLVLKQQNLPVTRILDNLMEMKSNPETDDYRYFDPKMLRSNDSSFLYKT
EEPPASQEGGPGIPS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC027793
ORF Size 1725 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC027793.1
RefSeq Size 3135 bp
RefSeq ORF 1727 bp
Locus ID 76983
Gene Summary Plays a role in SNARE-pin assembly and Golgi-to-ER retrograde transport via its interaction with COG4. Involved in vesicular transport between the endoplasmic reticulum and the Golgi (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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