Acsm3 (BC015248) Mouse Tagged ORF Clone
CAT#: MG209073
- TrueORF®
Acsm3 (tGFP-tagged) - Mouse acyl-CoA synthetase medium-chain family member 3 (cDNA clone MGC:18326 IMAGE:4239288)
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CNY 5,040.00
CNY 1,000.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | Sa; Sah |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG209073 representing BC015248
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTACTCCGTGCTAGGTGTTTTCAGCGCCTAGCAATTCCTGATCCTATGAGAGTCTTGTATAAAGATT ACAGAACAGCGACCCCTCAGAACTTTTCCAACTATGAGTCCATGAAACAAGACTTCAAAATAGAGATTCC AGAGTATTTCAATTTTGCAAAAGATGTCCTGGACCAATGGACCAATATGGAAAAGGCTGGAAAGAGACTT TCCAATCCAGCCTTCTGGTGGATAGATGGGAATGGAGAAGAGCTGAGATGGAGTTTTGAAGAACTTGGGT TGTTATCCAGGAAATTTGCCAACATACTCACAGAAGCCTGCTCCCTGCAAAGAGGAGACAGAGTAATGGT GATACTGCCCAAGATCCCAGAGTGGTGGCTTGCAAATGTGGCCTGTCTGCGAACAGGGACAGTTTTAATT CCAGGAACCACTCAGCTGACCCAGAAAGACATCCTCTATAGACTACAATCTTCAAAAGCTAAGTGCATTA TTACCGATGATACTTTGGCCCCAGCAGTAGATGCCGTGGCAGCTAAATGTGAAAATCTCCACTCCAAATT AATTGTGTCTCAGCACTCCAGAGAAGGCTGGGGGAACCTCAAGGAGATGATGAAATATGCCAGTGACAGC CACACTTGTGTGGACACAAAACACGACGAGATGATGGCCATCTACTTCACCAGTGGGACAACTGGGCCTC CTAAGATGATTGGACACACCCACAGCAGCTTTGGTTTAGGATTGTCTGTCAATGGAAGGTTCTGGCTGGA TTTGATAGCCTCCGATGTGATGTGGAATACTTCAGATACAGGCTGGGCAAAGTCTGCATGGAGTAGTGTT TTTTCTCCATGGACCCAAGGAGCATGTGTTTTTGCACACTATTTGCCCCGTTTTGAATCAACTTCCATCT TGCAAACCCTCTCCAAGTTCCCCATCACTGTCTTCTGTTCTGCACCAACTGCCTACCGGATGCTTGTTCA GAATGACATGAGCAGCTATAAGTTCAACAGTTTGAAGCACTGTGTCAGTGCTGGAGAACCTATTAACCCT GAAGTGATGGAACAATGGAGAAAGAAGACGGGCCTAGACATCTATGAAGGATATGGACAGACAGAAACGG TGCTGATCTGTGGAAATTTCAAGGGGATGAAAATTAAGCCCGGCTCAATGGGAAAGCCTTCTCCTGCTTT TGATGTGAAGATTTTAGATGAAAATGGTGCCACTCTTCCTCCTGGACAAGAAGGGGATATTGCTCTTCAA GTTCTTCCTGAGCGACCATTTGGCCTTTTTACTCATTATGTAGATAATCCTTCCAAAACGGCTTCAACTC TACGAGGGAGTTTCTACATTACTGGGGACAGAGGATATATGGATGAAGATGGCTATTTCTGGTTTGTTGC AAGATCAGATGATATCATATTATCTTCTGGTTACCGAATTGGACCATTTGAGGTAGAAAGTGCCCTCATA GAACATCCTTCCATCGCAGAGTCAGCTGTTGTCAGCAGTCCAGACCCCATCAGAGGAGAGGTAGTAAAGG CTTTTATTGTCCTGAATCCTGATTACAAGTCCCATGATCAAGAGCAACTGAAAAAGGAGATCCAGGAGCA CGTGAAGAAGACTACAGCGCCTTACAAATACCCCAGAAAGGTAGAATTTATTGAAGAGCTGCCGAAGACT GTCAGTGGGAAGGTCAAAAGAAATGAACTGAGGAAAAAGGAATGGGTAACAACT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG209073 representing BC015248
Red=Cloning site Green=Tags(s) MLLRARCFQRLAIPDPMRVLYKDYRTATPQNFSNYESMKQDFKIEIPEYFNFAKDVLDQWTNMEKAGKRL SNPAFWWIDGNGEELRWSFEELGLLSRKFANILTEACSLQRGDRVMVILPKIPEWWLANVACLRTGTVLI PGTTQLTQKDILYRLQSSKAKCIITDDTLAPAVDAVAAKCENLHSKLIVSQHSREGWGNLKEMMKYASDS HTCVDTKHDEMMAIYFTSGTTGPPKMIGHTHSSFGLGLSVNGRFWLDLIASDVMWNTSDTGWAKSAWSSV FSPWTQGACVFAHYLPRFESTSILQTLSKFPITVFCSAPTAYRMLVQNDMSSYKFNSLKHCVSAGEPINP EVMEQWRKKTGLDIYEGYGQTETVLICGNFKGMKIKPGSMGKPSPAFDVKILDENGATLPPGQEGDIALQ VLPERPFGLFTHYVDNPSKTASTLRGSFYITGDRGYMDEDGYFWFVARSDDIILSSGYRIGPFEVESALI EHPSIAESAVVSSPDPIRGEVVKAFIVLNPDYKSHDQEQLKKEIQEHVKKTTAPYKYPRKVEFIEELPKT VSGKVKRNELRKKEWVTT TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | BC015248 |
ORF Size | 1734 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | BC015248, AAH15248 |
RefSeq Size | 2737 bp |
RefSeq ORF | 1736 bp |
Locus ID | 20216 |
Gene Summary | Has medium-chain fatty acid:CoA ligase activity with broad substrate specificity (in vitro). Acts on acids from C(4) to C(11) and on the corresponding 3-hydroxy- and 2,3- or 3,4-unsaturated acids (in vitro).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |