Chuk (BC018243) Mouse Tagged ORF Clone

CAT＃: MG209133

  • TrueORF®

Chuk (tGFP-tagged) - Mouse conserved helix-loop-helix ubiquitous kinase (cDNA clone MGC:25325 IMAGE:4511275)

ORF Plasmid: DDK tGFP

Datasheet
SDS
Plasmid Map

  "BC018243" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 6,370.00

货期*
3周
规格
    • 10 ug

Product images

经常一起买 (4)
PS100010
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00

添加到购物车
TA150041
Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00

添加到购物车

【特别福利】购一抗正装产品（≥50ul或≥50ug），免费领10ul抗体试用装

CC100003
DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00

暂停销售
PRIMER
Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

添加到购物车

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms IKK1, Chuk1, IKK-1, IKK[a], IKK-alpha, IKKalpha
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG209133 representing BC018243
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCGGCCCCCGGGGCTGCGGCCGGGCGCGGGCGGCCCCTGGGAGATGCGGGAACGGCTTGGCACCG
GCGGTTTCGGGAACGTCAGTCTGTACCAGCACCGGGAACTTGATCTCAAAATAGCAATTAAGTCTTGTCG
TTTAGAGCTAAGTTCCAAAAACAGAGAGCGATGGTGCCATGAAATCCAGATCATGAAAAAGTTGGACCAT
GCGAATGTTGTAAAGGCCTGTGATGTCCCTGAGGAATTGAACTTTTTAATTAACGATGTGCCTCTTCTGG
CAATGGAGTACTGTTCTGGAGGGGACCTCCGGAAGCTACTCAACAAACCAGAAAATTGTTGTGGACTTAA
AGAAAGCCAGATACTTTCTTTACTGAGTGACATAGGATCTGGGATCCGATATCTGCATGAAAACAAAATT
ATACATCGAGATCTAAAACCTGAAAATATAGTTCTTCAAGATGTTGGTGGGAAGACAATACATAAAATAA
TTGATTTGGGTTATGCCAAAGATGTTGATCAAGGAAGTCTCTGTACATCTTTTGTGGGAACATTGCAGTA
TTTGGCCCCAGAGCTCTTTGAAAATAAGCCGTACACAGCCACTGTGGATTATTGGAGCTTTGGGACCATG
GTGTTTGAATGTATTGCTGGATATAGGCCTTTTTTGCATCATCTGCAGCCATTTACCTGGCATGAGAAGA
TTAAGAAGAAAGATCCAAAGTGTATATTTGCATGTGAAGAGATGACTGGAGAAGTTCGGTTTAGTAGCCA
TTTACCTCAGCCAAACAGCCTTTGTAGTTTAATAGTAGAGCCAATGGAAAGCTGGCTCCAATTGATGCTG
AATTGGGACCCACAGCAGAGAGGGGGACCTATTGATCTTACTTTGAAGCAGCCAAGATGTTTTGCATTAA
TGGATCACATTCTCAATTTAAAGATAGTGCACATCCTAAATATGACTTCTGCAAAAATCATTTCTTTTCT
GTTACCATGTGATGAAAGTCTTCATTCACTACAGTCTCGAATTGAGCGTGAAACAGGAATAAATACAGGT
TCTCAGGAGCTTCTGTCAGAGACAGGGATTTCTCTGGATCCTCGGAAACCAGCCTCTCAGTGTGTTCTAG
ATGGAGTTAGAGGCTGTGATAGCTACATGGTTTATTTGTTTGATAAAAGTAAGACTGTATATGAAGGACC
ATTTGCATCCAGAAGTTTATCTGATTGTGTAAATTATATTGTACAAGACAGCAAAATACAACTGCCAATT
ATACAGCTGCGGAAAGTATGGGCTGAAGCAGTGCACTACGTATCTGGGCTAAAGGAAGACTACAGCAGGC
TCTTCCAGGGACAAAGAGCAGCAATGTTAAGTCTTCTTAGATATAATGCTAACTTGACAAAAATGAAGAA
TACTTTGATCTCAGCATCACAGCAACTCAAAGCTAAATTGGAGTTTTTTCGAAAAAGCATTCAGCTTGAC
TTGGAGAGATATAGTGAGCAGATGACTTATGGGATATCTTCAGAAAAAATGTTAAAAGCATGGAAAGAAA
TGGAAGAAAAGGCCATTCACTATTCTGAGGTTGGTGTCATTGGTTATCTTGAGGATCAAATTATGTCTTT
GCACACTGAAATCATGGAGCTGCAGAAGAGCCCCTATGGACGACGCCAGGGAGACTTGATGGAGTCTCTG
GAGCAGCGTGCCATTGATCTCTATAAGCAGCTAAAGCACAGACCTCCTGGTAAGACACTTCAGTCACAGT
AT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG209133 representing BC018243
Red=Cloning site Green=Tags(s)

MERPPGLRPGAGGPWEMRERLGTGGFGNVSLYQHRELDLKIAIKSCRLELSSKNRERWCHEIQIMKKLDH
ANVVKACDVPEELNFLINDVPLLAMEYCSGGDLRKLLNKPENCCGLKESQILSLLSDIGSGIRYLHENKI
IHRDLKPENIVLQDVGGKTIHKIIDLGYAKDVDQGSLCTSFVGTLQYLAPELFENKPYTATVDYWSFGTM
VFECIAGYRPFLHHLQPFTWHEKIKKKDPKCIFACEEMTGEVRFSSHLPQPNSLCSLIVEPMESWLQLML
NWDPQQRGGPIDLTLKQPRCFALMDHILNLKIVHILNMTSAKIISFLLPCDESLHSLQSRIERETGINTG
SQELLSETGISLDPRKPASQCVLDGVRGCDSYMVYLFDKSKTVYEGPFASRSLSDCVNYIVQDSKIQLPI
IQLRKVWAEAVHYVSGLKEDYSRLFQGQRAAMLSLLRYNANLTKMKNTLISASQQLKAKLEFFRKSIQLD
LERYSEQMTYGISSEKMLKAWKEMEEKAIHYSEVGVIGYLEDQIMSLHTEIMELQKSPYGRRQGDLMESL
EQRAIDLYKQLKHRPPGKTLQSQY

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC018243
ORF Size 1754 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC018243, AAH18243
RefSeq Size 2056 bp
RefSeq ORF 1754 bp
Locus ID 12675
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

SKU Description Size Price
MC218087 Chuk (untagged) - Mouse conserved helix-loop-helix ubiquitous kinase (cDNA clone MGC:25325 IMAGE:4511275), (10ug)
    • 10 ug

CNY 6,940.00

MR209133 Chuk (Myc-DDK-tagged) - Mouse conserved helix-loop-helix ubiquitous kinase (cDNA clone MGC:25325 IMAGE:4511275)
    • 10 ug

CNY 4,352.00
CNY 5,800.00

MR209133L3 Lenti ORF clone of Chuk (Myc-DDK-tagged) - Mouse conserved helix-loop-helix ubiquitous kinase (cDNA clone MGC:25325 IMAGE:4511275)
    • 10 ug

CNY 6,752.00
CNY 7,700.00

MR209133L4 Lenti ORF clone of Chuk (mGFP-tagged) - Mouse conserved helix-loop-helix ubiquitous kinase (cDNA clone MGC:25325 IMAGE:4511275)
    • 10 ug

CNY 7,700.00

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    • 100 ul

CNY 1,000.00

MC218087

Chuk (untagged) - Mouse conserved helix-loop-helix ubiquitous kinase (cDNA clone MGC:25325 IMAGE:4511275), (10ug)

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CNY 6,940.00

MR209133L4V

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CNY 11,115.00 暂停销售

MR209133L3V

Lenti ORF particles, Chuk (Myc-DDK-tagged) - Mouse conserved helix-loop-helix ubiquitous kinase (cDNA clone MGC:25325 IMAGE:4511275), 200ul, >10^7 TU/mL

    • 200 ul

CNY 9,975.00 暂停销售

MR209133L4

Lenti ORF clone of Chuk (mGFP-tagged) - Mouse conserved helix-loop-helix ubiquitous kinase (cDNA clone MGC:25325 IMAGE:4511275)

    • 10 ug

CNY 7,700.00

MR209133L3

Lenti ORF clone of Chuk (Myc-DDK-tagged) - Mouse conserved helix-loop-helix ubiquitous kinase (cDNA clone MGC:25325 IMAGE:4511275)

    • 10 ug

CNY 6,752.00 CNY 7,700.00

MR209133

Chuk (Myc-DDK-tagged) - Mouse conserved helix-loop-helix ubiquitous kinase (cDNA clone MGC:25325 IMAGE:4511275)

    • 10 ug

CNY 4,352.00 CNY 5,800.00

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