Trim29 (BC027353) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	1110047J21Rik; 2810431N19Rik; 4732461M22Rik; AI119726
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG209159 representing BC027353 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGGTGCCGATGCCTGCAGGAGCAATGGGGCCAGCCCGGAAGCCAGGGACACCCGCAGCCCACCGG GTCCCAGCGGCAGCCTAGAGAATGGCACTAAAGCAGACAGTAAGGACACCAAGACAACCAACGGGCACAG TGGGGAGGTGACAGAGGGCAAGACCCTGGGCAGTGCCCTGAAGTCAGGGGAGGGGAAGAGTGGCCTATTT TCCAGCAATGAGTGGCGCAGGCCTATCATCCAGTTTGTGGAGTCTGTCGACGACAAAGGCTCCAGTTACT TCAGTATGGACTCTGCGGAAGGCAGGAGGTCCCCTTATGCAGGGCTCCAGTTGGGGGCTTCCAAGAAACC TCCTGTGACCTTTGCTGAGAAGGGGGAGCTGCGAAAGTCCATTTTCTCCGAGCCCCGCAAACCCACGGTG ACCATCGTGGAGCCCGGGGAAGTCCGCAGGAATAGCTACCCCAGGGCTGACTCCAGCCTCCTGGCGCGGG CAAAGTCGGGCTCCGAGGAGGTGCTGTGCGACTCCTGCATCGGCAACAAGCAGAAGGCCGTCAAGTCCTG CCTGGTGTGCCAGGCTTCCTTCTGTGAGCTGCATCTGAAGCCCCACCTGGAGGGCGCTGCCTTCCGGGAC CACCAGCTGCTCGAGCCCATCCGAGACTTCGAGGCCCGAAAATGTCCCTTGCACGGCAAGACTATGGAGC TCTTCTGTCAGACCGACCAGACCTGCATCTGTTATCTCTGCATGTTCCAGGAGCACAAGAACCACAGCAC CGTGACGGTGGAGGAGGCCAAGGCAGAGAAGGAGACCGAGCTGTCACTGCAAAAGGAACAGCTGCAACTG AAGATCATTGAGATTGAAGATGATGTTGAGAAGTGGCAAAAGGAGAAAGACCGGATCAAGAGCTTTACCA CCAATGAGAAGGCGATCCTGGAGCAGAACTTCAGGGACCTGGTGAGGGAGCTGGAGAAGCAGAAGGAGGA AGTACGGGCTGCACTGGAGCAGCGGGAGCAGGATGCTGTGGACCAAGTGAAGGTGATCGTGGATGCTCTG GATGAGAGGGCCAAGGTGCTGCACGAGGACAAGCAGACCCGAGAGCAGCTGCACAACATCAGTGACTCGG TGCTGTTCCTGCAGGAATTTGGTGCATTGATGAGCAATTACTCTCTCCCTCCACCTCTGCCCACCTACCA TGTCCTGCTGGAGGGAGAGGGGCTGGGACAGTCTCTTGGCAACTGCAAGGATGACCTGCTCAATGTGTGC ATGCGTCATGTTGAGAAGATGTGCAAGGCCGATTTGAGCCGCAACTTCATTGAGAGGAACCATATGGAAA ATGGTGGGGACCATCGCTACATGAACAGCTACACCAGCAGCTATGGGAACGAGTGGAGCACGCCTGACAC CATGAAAAGATACTCCATGTACCTCACGCCCAAGGGTGGGGGCCGGACATCCTACCAGCCATCCTCGCCT AGCCGCCTCTCCAAGGAGACCAACCAGAAGAATTTCAACAATCTGTATGGCACAAAAGGCAACTATACCT CCAGGGTCTGGGAGTACACATCCACGGTTCAGAATTCTGAGGACATGCCCACGGTGCAAGGCAACTCCTC CTTCTCCCTGAAAGGCTTTCCCTCCCTCCTCCGGAGCCAAGTTCCCAAGGCCCAGCCTCAGACTTGGAAA TCTGGCAAGCAGACTCTGCTGTCTCACTACCGGCCATTCTACGTCAACAAGGGCAGCGGCATCGGATCCA ACGAGGCGCCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG209159 representing BC027353 Red=Cloning site Green=Tags(s) MEGADACRSNGASPEARDTRSPPGPSGSLENGTKADSKDTKTTNGHSGEVTEGKTLGSALKSGEGKSGLF SSNEWRRPIIQFVESVDDKGSSYFSMDSAEGRRSPYAGLQLGASKKPPVTFAEKGELRKSIFSEPRKPTV TIVEPGEVRRNSYPRADSSLLARAKSGSEEVLCDSCIGNKQKAVKSCLVCQASFCELHLKPHLEGAAFRD HQLLEPIRDFEARKCPLHGKTMELFCQTDQTCICYLCMFQEHKNHSTVTVEEAKAEKETELSLQKEQLQL KIIEIEDDVEKWQKEKDRIKSFTTNEKAILEQNFRDLVRELEKQKEEVRAALEQREQDAVDQVKVIVDAL DERAKVLHEDKQTREQLHNISDSVLFLQEFGALMSNYSLPPPLPTYHVLLEGEGLGQSLGNCKDDLLNVC MRHVEKMCKADLSRNFIERNHMENGGDHRYMNSYTSSYGNEWSTPDTMKRYSMYLTPKGGGRTSYQPSSP SRLSKETNQKNFNNLYGTKGNYTSRVWEYTSTVQNSEDMPTVQGNSSFSLKGFPSLLRSQVPKAQPQTWK SGKQTLLSHYRPFYVNKGSGIGSNEAP TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC027353
ORF Size	1763 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC027353, AAH27353
RefSeq Size	2763 bp
RefSeq ORF	1763 bp
Locus ID	72169
Gene Summary	Plays a crucial role in the regulation of macrophage activation in response to viral or bacterial infections within the respiratory tract. Mechanistically, TRIM29 interacts with IKBKG/NEMO in the lysosome where it induces its 'Lys-48' ubiquitination and subsequent degradation. In turn, the expression of type I interferons and the production of proinflammatory cytokines are inhibited. Additionally, induces the 'Lys-48' ubiquitination of TMEM173/STING in a similar way, leading to its degradation.[UniProtKB/Swiss-Prot Function]

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