Mbd1 (BC069837) Mouse Tagged ORF Clone

CAT#: MG209180

  • TrueORF®

Mbd1 (tGFP-tagged) - Mouse methyl-CpG binding domain protein 1 (cDNA clone MGC:76676 IMAGE:30435760)



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Mouse Protein / Lysate Request

CNY 5,800.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms PCM1, Cxxc3
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG209180 representing BC069837
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGAGTCCTGGCAGGACTGCCCAGCCCTGGGCCCTGGCTGGAAACGCCGAGAGTCCTTTCGAAAGT
CAGGGGCCTCGTTTGGACGCTCAGACATCTATTACCAGAGCCCCACAGGAGAAAAGATCCGAAGCAAAGT
TGAGCTGACTCGGTACTTGGGCCCTGCATGTGATCTTACCCTGTTTGACTTCAGACAAGGCACCTTATGC
CATCCCATTCCCAAGACTCATCCCTTGGCTGTCCCCAGCAAGAAGAAAAAGAAGCCTTCTAAACCAGCCA
AGACTAAGAAACAGCAGGTTGGGCTGCAGCGAAGTGAGGTCAGGAGAGAGACTCCGCAGGGGGAATATAA
GGCCCCTACTGCCACAGCTCTAGCTTCACTATCTGTGTCTGCATCTGCGTCTTCGTCTGCATCTGCATCT
GCGTCTTCGTTTGCATCTGCATCTGCATCTGCGTCTTCACGTGCACCTGTGTGCTGTGAGAATTGTGGAA
TCCACTTTTCATGGGATGGTGTCAAAAGGCAGAGACTTAAGACGTTGTGCAAAGATTGTCGAGCACAGAG
AATCGCCTTCAACCGCGAACAGAGAATGTTTAAGCGAGTCGGCTGTGGGGACTGTGCAGCTTGCCTGGTG
AAAGAGGACTGTGGGGTGTGCTCCACCTGTCGCCTGCAACTGCCCAGTGATGTGGCCTCAGGGCTTTACT
GCAAGTGTGAGCGGAGACGATGCCTGCGGATTATGGAGAAGAGCCGAGGGTGTGGCGTGTGTCGGGGTTG
TCAGACCCAAGAGGACTGTGGCCATTGCTGCATCTGTCTTCGCTCTCCCCGCCCTGGTCTCAAGCGCCAG
TGGAGGTGTTTGCAACGGCGCTGTTTTTGGGGTAAACGTGATTCTAGTAAGAGAGGCTCGAAGGTAGCGT
CTCAGCGTCACTCCCAAGCCCCGCCACTTCCTCCACATCCTGCATCACAGTACACAGAGCCCACAGAGCT
GCACATCAGCGACATAGCACCCACATCACCTGCTGAGTTCATCTATTACTGTGTAGACGAGGACGAGGAC
GAGCTAAAGCGGCTGCTGCCCAGTGCTGGGTCAGGGTCTGGGGAGGGAGCAGGATTGCGTCCATATCAGA
CCCATCAGACCCATCAAAAGAGGCCTGCTTCTGCTCGACAACTCCAACTGAGCTCTCCCTTAAAGGCCCC
TTGGGCTGTGGTCACAGCACCACCAGGCCCTGTCCGAGATTCAAGAAAGCAGCAAGCAGGTAGAGGCTCT
GTACTGCCCCAGCCTGACACAGACTTTGTGTTTTTACAAGAGGGTACCAGCAGTGCCATGCAGATGCCTG
GCACTGCTGCAGCTTGCACAGAAGTCCCAGTACAGGCGGCCCAGTGCTCTGCCCCGAGCTGGGTTGTGGC
CTTACCCCAGGTGAAGCAAGAGACGGCAGATGCTCCAGAGGAGTGGACAGCAGTCACGACCTTCCTGACT
TCCTCCACGCTGCAGTCTGGCTTCCCTAGCAAGGCTGCAGATCCAGACCTTTCACCTGTGAAACAAGAGC
CCCCTGGCCCTGAAGAGGATGGAGAAGAGAAAAAAGATGATGTCTCTGAAACAACCCCAGCAGAGGAGAT
AGGAGGGGTTGGCACGCCAGTGATCACGGAGATTTTCAGCCTGGGTGGAACCCGCTTCCGAGATGCAGAA
GCCTGGTTGCCAAGGCTACATAAACTGTTAGCAGTAAATGAAAAAGAGTATTTTACTGAGCTGCAGTTGA
AAGAAGAAGTTTTG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG209180 representing BC069837
Red=Cloning site Green=Tags(s)

MAESWQDCPALGPGWKRRESFRKSGASFGRSDIYYQSPTGEKIRSKVELTRYLGPACDLTLFDFRQGTLC
HPIPKTHPLAVPSKKKKKPSKPAKTKKQQVGLQRSEVRRETPQGEYKAPTATALASLSVSASASSSASAS
ASSFASASASASSRAPVCCENCGIHFSWDGVKRQRLKTLCKDCRAQRIAFNREQRMFKRVGCGDCAACLV
KEDCGVCSTCRLQLPSDVASGLYCKCERRRCLRIMEKSRGCGVCRGCQTQEDCGHCCICLRSPRPGLKRQ
WRCLQRRCFWGKRDSSKRGSKVASQRHSQAPPLPPHPASQYTEPTELHISDIAPTSPAEFIYYCVDEDED
ELKRLLPSAGSGSGEGAGLRPYQTHQTHQKRPASARQLQLSSPLKAPWAVVTAPPGPVRDSRKQQAGRGS
VLPQPDTDFVFLQEGTSSAMQMPGTAAACTEVPVQAAQCSAPSWVVALPQVKQETADAPEEWTAVTTFLT
SSTLQSGFPSKAADPDLSPVKQEPPGPEEDGEEKKDDVSETTPAEEIGGVGTPVITEIFSLGGTRFRDAE
AWLPRLHKLLAVNEKEYFTELQLKEEVL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC069837
ORF Size 1766 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC069837, AAH69837
RefSeq Size 2736 bp
RefSeq ORF 1766 bp
Locus ID 17190
Gene Summary Transcriptional repressor that binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binding is abolished by the presence of 7-mG that is produced by DNA damage by methylmethanesulfonate (MMS). Acts as transcriptional repressor and plays a role in gene silencing by recruiting AFT7IP, which in turn recruits factors such as the histone methyltransferase SETDB1. Probably forms a complex with SETDB1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Isoform 1 can also repress transcription from unmethylated promoters.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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