Mbd1 (BC069837) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	PCM1, Cxxc3
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG209180 representing BC069837 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGAGTCCTGGCAGGACTGCCCAGCCCTGGGCCCTGGCTGGAAACGCCGAGAGTCCTTTCGAAAGT CAGGGGCCTCGTTTGGACGCTCAGACATCTATTACCAGAGCCCCACAGGAGAAAAGATCCGAAGCAAAGT TGAGCTGACTCGGTACTTGGGCCCTGCATGTGATCTTACCCTGTTTGACTTCAGACAAGGCACCTTATGC CATCCCATTCCCAAGACTCATCCCTTGGCTGTCCCCAGCAAGAAGAAAAAGAAGCCTTCTAAACCAGCCA AGACTAAGAAACAGCAGGTTGGGCTGCAGCGAAGTGAGGTCAGGAGAGAGACTCCGCAGGGGGAATATAA GGCCCCTACTGCCACAGCTCTAGCTTCACTATCTGTGTCTGCATCTGCGTCTTCGTCTGCATCTGCATCT GCGTCTTCGTTTGCATCTGCATCTGCATCTGCGTCTTCACGTGCACCTGTGTGCTGTGAGAATTGTGGAA TCCACTTTTCATGGGATGGTGTCAAAAGGCAGAGACTTAAGACGTTGTGCAAAGATTGTCGAGCACAGAG AATCGCCTTCAACCGCGAACAGAGAATGTTTAAGCGAGTCGGCTGTGGGGACTGTGCAGCTTGCCTGGTG AAAGAGGACTGTGGGGTGTGCTCCACCTGTCGCCTGCAACTGCCCAGTGATGTGGCCTCAGGGCTTTACT GCAAGTGTGAGCGGAGACGATGCCTGCGGATTATGGAGAAGAGCCGAGGGTGTGGCGTGTGTCGGGGTTG TCAGACCCAAGAGGACTGTGGCCATTGCTGCATCTGTCTTCGCTCTCCCCGCCCTGGTCTCAAGCGCCAG TGGAGGTGTTTGCAACGGCGCTGTTTTTGGGGTAAACGTGATTCTAGTAAGAGAGGCTCGAAGGTAGCGT CTCAGCGTCACTCCCAAGCCCCGCCACTTCCTCCACATCCTGCATCACAGTACACAGAGCCCACAGAGCT GCACATCAGCGACATAGCACCCACATCACCTGCTGAGTTCATCTATTACTGTGTAGACGAGGACGAGGAC GAGCTAAAGCGGCTGCTGCCCAGTGCTGGGTCAGGGTCTGGGGAGGGAGCAGGATTGCGTCCATATCAGA CCCATCAGACCCATCAAAAGAGGCCTGCTTCTGCTCGACAACTCCAACTGAGCTCTCCCTTAAAGGCCCC TTGGGCTGTGGTCACAGCACCACCAGGCCCTGTCCGAGATTCAAGAAAGCAGCAAGCAGGTAGAGGCTCT GTACTGCCCCAGCCTGACACAGACTTTGTGTTTTTACAAGAGGGTACCAGCAGTGCCATGCAGATGCCTG GCACTGCTGCAGCTTGCACAGAAGTCCCAGTACAGGCGGCCCAGTGCTCTGCCCCGAGCTGGGTTGTGGC CTTACCCCAGGTGAAGCAAGAGACGGCAGATGCTCCAGAGGAGTGGACAGCAGTCACGACCTTCCTGACT TCCTCCACGCTGCAGTCTGGCTTCCCTAGCAAGGCTGCAGATCCAGACCTTTCACCTGTGAAACAAGAGC CCCCTGGCCCTGAAGAGGATGGAGAAGAGAAAAAAGATGATGTCTCTGAAACAACCCCAGCAGAGGAGAT AGGAGGGGTTGGCACGCCAGTGATCACGGAGATTTTCAGCCTGGGTGGAACCCGCTTCCGAGATGCAGAA GCCTGGTTGCCAAGGCTACATAAACTGTTAGCAGTAAATGAAAAAGAGTATTTTACTGAGCTGCAGTTGA AAGAAGAAGTTTTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG209180 representing BC069837 Red=Cloning site Green=Tags(s) MAESWQDCPALGPGWKRRESFRKSGASFGRSDIYYQSPTGEKIRSKVELTRYLGPACDLTLFDFRQGTLC HPIPKTHPLAVPSKKKKKPSKPAKTKKQQVGLQRSEVRRETPQGEYKAPTATALASLSVSASASSSASAS ASSFASASASASSRAPVCCENCGIHFSWDGVKRQRLKTLCKDCRAQRIAFNREQRMFKRVGCGDCAACLV KEDCGVCSTCRLQLPSDVASGLYCKCERRRCLRIMEKSRGCGVCRGCQTQEDCGHCCICLRSPRPGLKRQ WRCLQRRCFWGKRDSSKRGSKVASQRHSQAPPLPPHPASQYTEPTELHISDIAPTSPAEFIYYCVDEDED ELKRLLPSAGSGSGEGAGLRPYQTHQTHQKRPASARQLQLSSPLKAPWAVVTAPPGPVRDSRKQQAGRGS VLPQPDTDFVFLQEGTSSAMQMPGTAAACTEVPVQAAQCSAPSWVVALPQVKQETADAPEEWTAVTTFLT SSTLQSGFPSKAADPDLSPVKQEPPGPEEDGEEKKDDVSETTPAEEIGGVGTPVITEIFSLGGTRFRDAE AWLPRLHKLLAVNEKEYFTELQLKEEVL TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC069837
ORF Size	1764 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC069837, AAH69837
RefSeq Size	2736 bp
RefSeq ORF	1766 bp
Locus ID	17190
Gene Summary	Transcriptional repressor that binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binding is abolished by the presence of 7-mG that is produced by DNA damage by methylmethanesulfonate (MMS). Acts as transcriptional repressor and plays a role in gene silencing by recruiting AFT7IP, which in turn recruits factors such as the histone methyltransferase SETDB1. Probably forms a complex with SETDB1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Isoform 1 can also repress transcription from unmethylated promoters.[UniProtKB/Swiss-Prot Function]

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