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Ythdf3 (BC057158) Mouse Tagged ORF Clone
CAT＃: MG209195
- TrueORF®
Ythdf3 (tGFP-tagged) - Mouse YTH domain family 3 (cDNA clone MGC:67692 IMAGE:5348717)
ORF Plasmid: DDK
"BC057158" in other vectors (4)
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CNY 6,460.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 9130022A11Rik |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG209195 representing BC057158
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCTATCTTGATTTGACTTTGCTTCATAGGGCAACAGAGGAAACAGGCGAAGAATCATTTTCAGTAC AAAATGGTTCGATTCATCAAAAAGATGCTGTAAATGATGATGATTTTGAGCCATACTTAAGTAGCCAGAC AAATCAGAATAACAGCTATCCACCAATGTCAGATCCATATATGCCTAGTTACTATGCTCCGTCCATTGGA TTTCCATATTCTCTTGGGGAAGCAGCATGGTCCACGGCTGGAGATCAGCCTATGCCATATCTGACAACCT ATGGACAAATGAGTAATGGAGAACATCATTATATACCAGATGGTGTTTTTAGTCAACCAGGGGCATTAGG AAATACCCCTCCATTTCTTGGTCAACATGGATTTAACTTTTTTCCTGGTAATGCTGATTTCTCTACATGG GGAACAAGTGGATCTCAGGGACAATCAACACAAAATTCTGCTTATAGTAGCAGTTACGGCTATCCACCTA GTTCTCTTGGGAGAGCTATTACTGATGGACAGGCCGGATTTGGCAATGATACTTTGAGTAAGGTGCCTGG CATTAGTAGTATTGAGCAAGGCATGACTGGACTGAAAATTGGTGGTGACCTGACAGCTGCGGTGACGAAA ACTGTAGGTACAGCCTTGAGCAGCAGTGGTATGACTAGCATTGCAACCAATAATGTGCCCCCTGTTAGCA GTGCAGCACCTAAACCAACTTCTTGGGCTGCTATTGCCAGAAAGCCTGCAAAACCTCAGCCGAAACTTAA ACCCAAGGGCAATGTGGGAATTGGGGGTTCTGCTGTGCCACCACCTCCTATAAAACACAACATGAATATT GGAACTTGGGATGAAAAGGGGTCAGTGGTAAAGGCTCCACCAACCCAACCAGTTCTGCCTCCTCAAACTA TAATCCAGCAGCCTCAGCCATTAATTCAACCACCACCATTGGTGCAAAGCCAACTGCCTCAACAGCAGCC TCAGCCACCACAACCACAGCAGCAACAAGGACCTCAGCCACAGGCCCAGCCTCACCAAGTGCAGTCTCAA CAGCCACAGTTGCAGAATCGTTGGGTAGCTCCTCGGAATAGGGGAACCGGCTTCAACCAGAACAATGGAA CAGGCAGTGAAAACTTTGGTTTAGGTGTTGTGCCTGTTAGTGCTTCACCTTCTAGTGTAGAGGTGCATCC AGTGCTGGAAAAACTAAAGGCCATAAACAATTATAATCCCAAAGACTTTGATTGGAACCTGAAGAATGGA CGTGTGTTTATAATTAAGAGCTATTCTGAGGATGATATACATCGTTCCATCAAGTACTCTATCTGGTGTA GTACTGAGCATGGTAATAAGCGTTTGGATGCAGCTTATCGTTCCCTGAATGGGAAAGGCCCACTCTATTT ACTCTTCAGTGTGAATGGCAGTGGACATTTTTGTGGAGTGGCTGAAATGAAGTCTGTTGTAGACTATAAT GCTTATGCTGGTGTCTGGTCTCAGGATAAGTGGAAGGGCAAATTTGAAGTTAAATGGATCTTTGTCAAAG ATGTTCCCAATAACCAATTACGACATATTCGCTTAGAAAATAATGACAACAAACCAGTAACCAATTCAAG GGACACTCAAGAAGTACCCCTAGAAAAAGCAAAGCAAGTGCTTAAAATAATCGCTACTTTCAAGCATACC ACCTCAATCTTTGATGACTTTGCACATTATGAAAAGCGTCAAGAGGAGGAGGAAGCCATGCGAAGGGAGA GAAATAGAAACAAGCAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG209195 representing BC057158
Red=Cloning site Green=Tags(s) MFYLDLTLLHRATEETGEESFSVQNGSIHQKDAVNDDDFEPYLSSQTNQNNSYPPMSDPYMPSYYAPSIG FPYSLGEAAWSTAGDQPMPYLTTYGQMSNGEHHYIPDGVFSQPGALGNTPPFLGQHGFNFFPGNADFSTW GTSGSQGQSTQNSAYSSSYGYPPSSLGRAITDGQAGFGNDTLSKVPGISSIEQGMTGLKIGGDLTAAVTK TVGTALSSSGMTSIATNNVPPVSSAAPKPTSWAAIARKPAKPQPKLKPKGNVGIGGSAVPPPPIKHNMNI GTWDEKGSVVKAPPTQPVLPPQTIIQQPQPLIQPPPLVQSQLPQQQPQPPQPQQQQGPQPQAQPHQVQSQ QPQLQNRWVAPRNRGTGFNQNNGTGSENFGLGVVPVSASPSSVEVHPVLEKLKAINNYNPKDFDWNLKNG RVFIIKSYSEDDIHRSIKYSIWCSTEHGNKRLDAAYRSLNGKGPLYLLFSVNGSGHFCGVAEMKSVVDYN AYAGVWSQDKWKGKFEVKWIFVKDVPNNQLRHIRLENNDNKPVTNSRDTQEVPLEKAKQVLKIIATFKHT TSIFDDFAHYEKRQEEEEAMRRERNRNKQ TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC057158 |
| ORF Size | 1769 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | BC057158, AAH57158 |
| RefSeq Size | 3173 bp |
| RefSeq ORF | 1769 bp |
| Locus ID | 229096 |
| Gene Summary | Specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs and promotes RNA translation efficiency (By similarity). M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability (By similarity). Shares m6A-containing mRNAs targets with YTHDF1 and YTHDF2, and regulates different processes depending on the context (By similarity). Facilitates the translation of targeted mRNAs in cooperation with YTHDF1 by binding to m6A-containing mRNAs and interacting with 40S and 60S ribosome subunits (By similarity). Acts as a negative regulator of type I interferon response by down-regulating interferon-stimulated genes (ISGs) expression: acts by binding to FOXO3 mRNAs and promoting their translation (PubMed:30591559). Binds to FOXO3 mRNAs independently of METTL3-mediated m6A modification (PubMed:30591559). Can also act as a regulator of mRNA stability in cooperation with YTHDF2 by binding to m6A-containing mRNA and promoting their degradation (By similarity). Recognizes and binds m6A-containing circular RNAs (circRNAs) and promotes their translation (By similarity). circRNAs are generated through back-splicing of pre-mRNAs, a non-canonical splicing process promoted by dsRNA structures across circularizing exons (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218605 | Ythdf3 (untagged) - Mouse YTH domain family 3 (cDNA clone MGC:67692 IMAGE:5348717), (10ug) |
CNY 6,940.00 |
|
| MR209195 | Ythdf3 (Myc-DDK-tagged) - Mouse YTH domain family 3 (cDNA clone MGC:67692 IMAGE:5348717) |
CNY 5,890.00 |
|
| MR209195L3 | Lenti ORF clone of Ythdf3 (Myc-DDK-tagged) - Mouse YTH domain family 3 (cDNA clone MGC:67692 IMAGE:5348717) |
CNY 7,790.00 |
|
| MR209195L4 | Lenti ORF clone of Ythdf3 (mGFP-tagged) - Mouse YTH domain family 3 (cDNA clone MGC:67692 IMAGE:5348717) |
CNY 7,790.00 |
