Cenpb (BC053333) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	CENP-B
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG209297 representing BC053333, codon optimized. Due to the complexity of BC053333, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGCCTAAAAGGAGACAACTTACCTTCAGAGAGAAGTCTAGGATCATTCAGGAGGTTGAGGAGAACC CTGACCTCCGCAAGGGTGAGATCGCTAGACGATTTAATATCCCACCCTCCACCCTGAGCACTATACTCAA AAATAAGCGGGCAATTCTGGCTTCCGAGCGCAAGTACGGAGTTGCTAGTACGTGTCGGAAGACGAACAAG CTGAGCCCATACGACAAACTGGAGGGGCTGTTGATCGCCTGGTTTCAACAGATTCGGGCTGCTGGACTTC CAGTTAAGGGAATTATTTTGAAGGAAAAGGCACTGCGCATCGCTGAGGAGCTCGGGATGGATGACTTCAC AGCCAGCAACGGTTGGCTGGATAGATTTAGGAGAAGGCACGGCGTGGTGGCTTGCTCAGGCGTGACTAGG AGTAGGGCACGGTCTAGCGCTCCGCGCGCTCCTGCTGCCCCGGCCGGACCCGCAACTGTCCCATCAGAAG GGTCCGGCGGGTCTACCCCGGGGTGGCATACCCGCGAGGAACAGCCTCCGTCAGTGGCAGAGGGGTATGC ATCTCAAGATGTGTTTTCAGCCACCGAGACATCTTTGTGGTACGATTTTCTGTCTGACCAAGCTTCAGGC CTCTGGGGCGGTGACGGCCCGGCCAGACAGGCTACTCAGAGACTGTCTGTCTTGCTTTGTGCCAATGCAG ACGGGAGCGAGAAGTTGCCTCCACTGGTGGCCGGTAAGAGTGCGAAACCAAGGGCAGGGCAAGGCGGGCT CCCATGTGACTACACCGCCAATTCCAAAGGGGGTGTGACCACGCAGGCTCTCGCTAAGTACTTGAAGGCG CTCGACACTAGAATGGCAGCTGAGTCCAGGAGAGTCCTGCTGCTGGCTGGACGCCTGGCAGCCCAGAGTT TGGATACAAGTGGACTCAGACACGTCCAGCTCGCGTTTTTTCCCCCCGGCACGGTTCACCCCCTTGAACG GGGGGTGGTTCAACAGGTCAAAGGGCACTACCGGCAGGCAATGCTCTTGAAGGCCATGGCCGCACTGGAG GGGCAGGATCCTTCAGGACTCCAGCTGGGATTGGTTGAGGCCCTCCACTTTGTGGCAGCAGCCTGGCAGG CCGTGGAGCCATCTGATATTGCAACATGTTTTCGCGAGGCCGGCTTCGGAGGAGGCCTTAACGCGACAAT TACCACCAGTTTTAAGTCCGAAGGAGAGGAGGAAGAGGAGGAGGAGGAGGAGGAAGAGGAAGAAGAAGAG GAGGAAGGTGAAGGAGAGGAAGAGGAGGAAGAGGAGGAGGAGGGCGAGGAGGAGGGAGGCGAAGGAGAAG AAGAGGGCGAGGAAGAGGTCGAAGAAGAGGGTGAAGTTGACGACAGTGACGAGGAAGAGGAGGAGAGCAG CAGTGAGGGCCTGGAGGCAGAGGATTGGGCTCAGGGCGTGGTCGAGGCAAGCGGTGGGTTCGGGGGCTAT AGTGTCCAAGAGGAGGCCCAGTTCCCAACCCTGCACTTTCTCGAGGGCGGCGAGGATTCTGATTCAGATT CAGATGAGGAAGAGGATGATGAGGAAGAGGATGAAGAGGATGAGGATGAGGAGGATGATGAGGATGGAGA TGAGGTTCCAGTCCCCAGCTTCGGAGAGGCGATGGCATACTTCGCTATGGTGAAGCGCTATCTCACCAGC TTCCCAATAGATGACCGGGTGCAGTCTCACATCCTGCACCTCGAGCACGACCTGGTCCACGTGACTAGGA AGAACCATGCCCGGCAAGCAGGCGTACGCGGGCTGGGTCATCAGAGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG209297 representing BC053333 Red=Cloning site Green=Tags(s) MGPKRRQLTFREKSRIIQEVEENPDLRKGEIARRFNIPPSTLSTILKNKRAILASERKYGVASTCRKTNK LSPYDKLEGLLIAWFQQIRAAGLPVKGIILKEKALRIAEELGMDDFTASNGWLDRFRRRHGVVACSGVTR SRARSSAPRAPAAPAGPATVPSEGSGGSTPGWHTREEQPPSVAEGYASQDVFSATETSLWYDFLSDQASG LWGGDGPARQATQRLSVLLCANADGSEKLPPLVAGKSAKPRAGQGGLPCDYTANSKGGVTTQALAKYLKA LDTRMAAESRRVLLLAGRLAAQSLDTSGLRHVQLAFFPPGTVHPLERGVVQQVKGHYRQAMLLKAMAALE GQDPSGLQLGLVEALHFVAAAWQAVEPSDIATCFREAGFGGGLNATITTSFKSEGEEEEEEEEEEEEEEE EEGEGEEEEEEEEEGEEEGGEGEEEGEEEVEEEGEVDDSDEEEEESSSEGLEAEDWAQGVVEASGGFGGY SVQEEAQFPTLHFLEGGEDSDSDSDEEEDDEEEDEEDEDEEDDEDGDEVPVPSFGEAMAYFAMVKRYLTS FPIDDRVQSHILHLEHDLVHVTRKNHARQAGVRGLGHQS TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC053333
ORF Size	1797 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC053333, AAH53333
RefSeq Size	2809 bp
RefSeq ORF	1799 bp
Locus ID	12616
Gene Summary	Interacts with centromeric heterochromatin in chromosomes and binds to a specific 17 bp subset of alphoid satellite DNA, called the CENP-B box. May organize arrays of centromere satellite DNA into a higher-order structure which then directs centromere formation and kinetochore assembly in mammalian chromosomes.[UniProtKB/Swiss-Prot Function]

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