Tdp1 (BC065162) Mouse Tagged ORF Clone

CAT#: MG209388

  • TrueORF®

Tdp1 (tGFP-tagged) - Mouse tyrosyl-DNA phosphodiesterase 1 (cDNA clone MGC:90084 IMAGE:6402921)



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Mouse Protein / Lysate Request

CNY 5,040.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms 2810481F14Rik; 4921509N21Rik; AI838772; AW493413; E430034L06Rik; MLZ-501; SCAN1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG209388 representing BC065162
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCCAAGAAAGCAGCTACGGGAAGTGGACAATATCCAGTAGTGACGAAAGCGAGGATGAGAAGCCCA
AACCTGATAAGCCGTCAGCATCCTCTCACCCCCAGGCTGGGCAGGGAGTGTCGAAGGAGCTCATATACAC
CTGCTCAGAGGCCCGCAAGGTTGCACACAAGAGACAGATCTCACCTGTGAAGTTCAACGACGCAGACTCG
GTGCTACCCCACAAGAAGCAGAAGAGTGACTCCCCGGAAGGTCTCGGCTGGTGTCTCTCCAGCAGCGATG
ATGACCAGCAGCCAGATGTGACACAGCAGGAACAGCCTAAGAGAGTGCTGCCTCAAGAGAAAAAACATGT
CTCCTCATCCGATGTGACTACTGCCCAGAAAGTTGTGGATAGAAGCCCTCCTGCCAGCCTTAGGCCCCAA
AGGGCAGACGATGAGTACGAGACCTCAGGGGAAGGCCAGGACATCTGGGACATGCTGGATAAGGGGAACC
CCTTCCAGTTTTATCTCACTAGAGTGTCGGGGATTAAGGCAAAGTACAACTCCAAAGCCCTCCACATCAA
GGATATTTTATCCCCTCTGTTTGGGACACTTGTCTCTTCAGCTCAGTTTAACTACTGCTTTGACGTGGAC
TGGCTCATAAAGCAATATCCACCGGAATTCAGGAAGAACCCGATCCTGCTTGTGCACGGTGATAAGAGGG
AAGCCAAGGCAGACTTACATGCCCAGGCGAAGCCCTATGCGAACATTTCCCTCTGCCAGGCAAAGTTGGA
TATTGCATTTGGAACACACCACACGAAAATGATGCTCTTGCTCTATGAAGAAGGCCTCCGAGTTGTCATC
CACACCTCCAACCTCATCCGGGAAGACTGGCACCAGAAAACCCAAGGAATATGGTTGAGCCCTTTGTACC
CAAGAATTGACCAGGGAAGCCACACAGCTGGAGAGTCAAGCACCCGTTTCAAAGCTGACCTCACCAGTTA
TCTAACGGCGTATAACGCTCCCCCTCTGCAGGAGTGGATAGACATCATCCAAGAGCATGACCTCTCTGAG
ACCAATGTTTACCTCATTGGTTCAACCCCTGGACGCTTTCAAGGAAGTCACAGAGATAACTGGGGACATT
TTAGGCTTAGAAAGCTTCTGCAAGCGCATGCCCCGTCCACACCCAAGGGCGAGTGCTGGCCTATCGTGGG
TCAGTTCTCAAGCATCGGCTCCCTGGGGCCCGATGAATCCAAGTGGCTGTGCTCGGAGTTTAAAGACAGC
TTGCTGGCACTGAGAGAGGAAGGCCGACCTCCGGGCAAGAGTGCAGTTCCTCTTCATCTGATCTATCCCT
CTGTGGAAAATGTGCGGACCAGCTTAGAAGGATACCCTGCTGGGGGCTCTCTTCCCTATAGCATCCAGAC
GGCTGAAAAGCAGAGGTGGCTGCACTCCTACTTCCACAAGTGGTCAGCAGAGACATCCGGCCGAAGTAAT
GCCATGCCACACATTAAGACGTACATGAGGCCCTCGCCAGACTTCAGTAAACTGGCTTGGTTCCTGGTCA
CAAGTGCGAATCTGTCCAAGGCTGCCTGGGGGGCACTGGAGAAGAACGGCACCCAGCTGATGATACGCTC
CTACGAGCTCGGGGTCCTCTTCCTCCCGTCTGCGTTTGGCCTGGACACCTTCAAAGTGAAACAGAAATTC
TTCTCCAGCAGCTGTGAGCCGACGGCCTCCTTTCCTGTGCCCTATGACCTGCCCCCAGAGCTGTACGGGA
GTAAAGACCGGCCTTGGATTTGGAACATTCCTTATGTCAAAGCACCTGACACGCACGGCAACATGTGGGT
GCCCTCC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG209388 representing BC065162
Red=Cloning site Green=Tags(s)

MSQESSYGKWTISSSDESEDEKPKPDKPSASSHPQAGQGVSKELIYTCSEARKVAHKRQISPVKFNDADS
VLPHKKQKSDSPEGLGWCLSSSDDDQQPDVTQQEQPKRVLPQEKKHVSSSDVTTAQKVVDRSPPASLRPQ
RADDEYETSGEGQDIWDMLDKGNPFQFYLTRVSGIKAKYNSKALHIKDILSPLFGTLVSSAQFNYCFDVD
WLIKQYPPEFRKNPILLVHGDKREAKADLHAQAKPYANISLCQAKLDIAFGTHHTKMMLLLYEEGLRVVI
HTSNLIREDWHQKTQGIWLSPLYPRIDQGSHTAGESSTRFKADLTSYLTAYNAPPLQEWIDIIQEHDLSE
TNVYLIGSTPGRFQGSHRDNWGHFRLRKLLQAHAPSTPKGECWPIVGQFSSIGSLGPDESKWLCSEFKDS
LLALREEGRPPGKSAVPLHLIYPSVENVRTSLEGYPAGGSLPYSIQTAEKQRWLHSYFHKWSAETSGRSN
AMPHIKTYMRPSPDFSKLAWFLVTSANLSKAAWGALEKNGTQLMIRSYELGVLFLPSAFGLDTFKVKQKF
FSSSCEPTASFPVPYDLPPELYGSKDRPWIWNIPYVKAPDTHGNMWVPS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC065162
ORF Size 1829 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC065162, AAH65162
RefSeq Size 2043 bp
RefSeq ORF 1829 bp
Locus ID 104884
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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