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Cenpb (BC006628) Mouse Tagged ORF Clone
CAT＃: MG209504
- TrueORF®
Cenpb (tGFP-tagged) - Mouse centromere protein B (cDNA clone MGC:11737 IMAGE:3968847)
ORF Plasmid: DDK
"BC006628" in other vectors (4)
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CNY 6,840.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CENP-B |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG209504 representing BC006628
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCCCCAAGCGGCGGCAGCTGACGTTCCGGGAGAAGTCGCGGATCATCCAGGAGGTGGAGGAGAACC CGGACCTACGCAAGGGCGAGATCGCGCGGCGCTTCAACATCCCGCCGTCCACGCTGAGCACCATCCTGAA GAACAAGCGCGCCATCCTGGCGTCGGAGCGCAAGTACGGAGTGGCCTCCACCTGCCGCAAGACCAACAAG CTGTCCCCGTACGACAAGCTGGAGGGGCTTCTCATCGCTTGGTTCCAGCAGATCCGCGCCGCCGGGCTGC CGGTCAAGGGCATCATCCTGAAAGAGAAGGCGCTGCGGATAGCGGAGGAGCTGGGCATGGACGACTTCAC TGCTTCCAACGGCTGGCTGGATCGCTTCCGCAGGCGCCACGGTGTAGTGGCCTGCAGCGGCGTGACCCGT TCTCGGGCGCGAAGCTCTGCCCCTCGGGCTCCAGCGGCACCTGCCGGCCCAGCCACGGTGCCCTCCGAGG GCAGCGGTGGGAGCACACCCGGCTGGCACACGAGGGAGGAGCAGCCGCCGTCGGTAGCTGAGGGCTACGC CTCGCAGGACGTGTTCAGCGCCACCGAGACCAGCCTGTGGTACGACTTTCTGTCGGACCAGGCCTCAGGG CTCTGGGGAGGTGATGGACCGGCGCGCCAGGCCACCCAGCGCCTTAGCGTTTTGCTGTGCGCCAACGCCG ATGGCAGCGAGAAGCTTCCCCCACTGGTCGCAGGCAAATCTGCCAAGCCCCGTGCAGGGCAAGGTGGTCT GCCCTGCGACTACACTGCCAACTCGAAGGGTGGAGTCACCACCCAGGCTCTGGCTAAGTACTTGAAAGCT CTGGACACTCGAATGGCAGCAGAATCTCGTCGGGTCCTTCTGCTGGCCGGCCGTCTGGCTGCCCAGTCCT TGGACACCTCGGGCCTGCGGCACGTGCAGCTGGCCTTCTTCCCTCCTGGCACCGTGCATCCTTTGGAGCG AGGAGTGGTCCAACAGGTGAAGGGCCACTACCGCCAGGCGATGTTGCTCAAGGCCATGGCAGCACTCGAG GGCCAGGATCCCTCAGGCTTGCAACTGGGCCTTGTGGAGGCCCTGCACTTTGTGGCTGCAGCCTGGCAGG CAGTGGAGCCCTCGGACATAGCAACTTGCTTTCGTGAGGCGGGTTTTGGAGGTGGCCTTAATGCCACTAT CACCACTTCCTTCAAGAGTGAGGGAGAGGAAGAGGAGGAAGAGGAGGAGGAAGAAGAGGAGGAGGAGGAA GAGGAGGGTGAAGGGGAAGAGGAGGAGGAAGAGGAAGAAGAAGGGGAGGAGGAAGGAGGGGAAGGAGAGG AGGAAGGAGAGGAAGAGGTAGAAGAGGAAGGTGAGGTTGATGATAGTGATGAAGAGGAGGAAGAAAGTTC CTCTGAGGGTTTAGAGGCTGAAGACTGGGCCCAGGGAGTAGTGGAAGCCAGTGGTGGTTTTGGGGGTTAT AGTGTCCAGGAAGAGGCCCAGTTCCCCACTCTCCATTTCCTGGAAGGAGGAGAGGACTCTGACTCAGACA GTGATGAAGAGGAGGACGATGAAGAGGAGGATGAGGAGGATGAAGATGAGGAGGATGATGAGGATGGTGA TGAGGTCCCTGTGCCCAGCTTTGGGGAGGCCATGGCTTACTTTGCCATGGTCAAGAGGTACCTGACCTCC TTCCCCATCGATGACCGAGTGCAGAGTCACATCCTTCATTTGGAACACGATCTGGTCCACGTGACTAGGA AGAACCGAGCCTGCCTCTGCCTGCTTTCCACCTCCCCAGATCCCTTTCTCTGGCCCAGTCTTTGGCCCTT GGTTTTCTTTCCAGATTGGAGGTTTCCAGGAGGCCCTCCAGGGGCG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG209504 representing BC006628
Red=Cloning site Green=Tags(s) MGPKRRQLTFREKSRIIQEVEENPDLRKGEIARRFNIPPSTLSTILKNKRAILASERKYGVASTCRKTNK LSPYDKLEGLLIAWFQQIRAAGLPVKGIILKEKALRIAEELGMDDFTASNGWLDRFRRRHGVVACSGVTR SRARSSAPRAPAAPAGPATVPSEGSGGSTPGWHTREEQPPSVAEGYASQDVFSATETSLWYDFLSDQASG LWGGDGPARQATQRLSVLLCANADGSEKLPPLVAGKSAKPRAGQGGLPCDYTANSKGGVTTQALAKYLKA LDTRMAAESRRVLLLAGRLAAQSLDTSGLRHVQLAFFPPGTVHPLERGVVQQVKGHYRQAMLLKAMAALE GQDPSGLQLGLVEALHFVAAAWQAVEPSDIATCFREAGFGGGLNATITTSFKSEGEEEEEEEEEEEEEEE EEGEGEEEEEEEEEGEEEGGEGEEEGEEEVEEEGEVDDSDEEEEESSSEGLEAEDWAQGVVEASGGFGGY SVQEEAQFPTLHFLEGGEDSDSDSDEEEDDEEEDEEDEDEEDDEDGDEVPVPSFGEAMAYFAMVKRYLTS FPIDDRVQSHILHLEHDLVHVTRKNRACLCLLSTSPDPFLWPSLWPLVFFPDWRFPGGPPGA TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC006628 |
| ORF Size | 1866 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC006628.1 |
| RefSeq Size | 2090 bp |
| RefSeq ORF | 1868 bp |
| Locus ID | 12616 |
| Gene Summary | Interacts with centromeric heterochromatin in chromosomes and binds to a specific 17 bp subset of alphoid satellite DNA, called the CENP-B box. May organize arrays of centromere satellite DNA into a higher-order structure which then directs centromere formation and kinetochore assembly in mammalian chromosomes.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC200749 | Cenpb (untagged) - Mouse centromere protein B (cDNA clone MGC:11737 IMAGE:3968847), (10ug) |
CNY 4,640.00 |
|
| MR209504 | Cenpb (Myc-DDK-tagged) - Mouse centromere protein B (cDNA clone MGC:11737 IMAGE:3968847) |
CNY 6,180.00 |
|
| MR209504L3 | Lenti ORF clone of Cenpb (Myc-DDK-tagged) - Mouse centromere protein B (cDNA clone MGC:11737 IMAGE:3968847) |
CNY 8,080.00 |
|
| MR209504L4 | Lenti ORF clone of Cenpb (mGFP-tagged) - Mouse centromere protein B (cDNA clone MGC:11737 IMAGE:3968847) |
CNY 8,080.00 |
