Pde1c (BC032277) Mouse Tagged ORF Clone

CAT#: MG209609

  • TrueORF®

Pde1c (tGFP-tagged) - Mouse phosphodiesterase 1C (cDNA clone MGC:40631 IMAGE:5400689)



Need custom modification / cloning service?
Get a free quote

Mouse Protein / Lysate Request

CNY 5,040.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG209609 representing BC032277
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGTCTCCAACCAAGGAAATTGAAGAATTCGAGAGCAACTCTCTGAAACACCTGCAACCTGAGCAGA
TCGAGAAAATCTGGCTTCGGCTCCGCGGGCTGAGGAAATATAAGAAAACGTCCCAGAGGTTACGGTCTTT
GGTCAAGCAATTAGAACGAGGAGAGGCCTCTGTGGTAGACCTTAAGAAGAATCTGGAATATGCAGCCACA
GTGCTTGAATCTGTATATATTGATGAAACAAGGCGACTGCTGGATACAGAGGATGAGCTTAGTGACATTC
AATCAGATGCTGTGCCTTCAGAAGTTCGGGATTGGCTGGCCTCCACCTTCACACGGCAGATGGGGATGAT
GCTTAGGAGAAGTGATGAGAAGCCCAGGTTCAAGAGCATCGTCCATGCGGTGCAAGCTGGGATATTTGTG
GAAAGAATGTACAGACGGACATCAAACATGGTTGGGCTGAGCTATCCACCGGCTGTAATCGATGCATTGA
AGGATGTGGATACGTGGTCCTTCGATGTCTTTTCCCTCAATGAGGCCAGTGGAGATCATGCACTGAAGTT
CATTTTCTATGAATTACTCACACGTTATGACCTGATCAGCCGTTTTAAGATACCGATTTCTGCACTTGTC
TCATTTGTGGAGGCCCTCGAAGTGGGTTACAGCAAGCACAAAAATCCTTACCATAACCTGATGCATGCAG
CTGACGTCACACAGACTGTGCATTACCTCCTTTATAAGACAGGAGTAGCAAACTGGCTGACAGAGCTGGA
GATCTTTGCAATAATCTTTTCGGCTGCCATCCATGACTATGAACATACTGGAACTACAAACAATTTCCAC
ATTCAGACTCGGTCAGATCCAGCTATCCTGTACAATGACAGATCTGTCCTGGAGAACCACCACCTGAGTG
CAGCCTACCGCCTTCTGCAGGAAGACGAAGAGATGAATATTCTGGTTAACCTCTCAAAGGATGACTGGAG
GGAGTTTCGAACTTTGGTTATTGAGATGGTAATGGCCACAGATATGTCCTGTCATTTCCAGCAAATCAAA
GCCATGAAGACAGCCCTGCAGCAACCAGAAGCAATTGAGAAGCCGAAAGCCTTATCCCTCATGTTACACA
CAGCAGACATCAGTCATCCTGCGAAAGCATGGGACCTGCACCACCGCTGGACCATGTCTCTCCTGGAGGA
GTTCTTTAGACAGGGTGACAGAGAAGCAGAGCTGGGGCTGCCATTTTCTCCTCTTTGTGACAGAAAGTCA
ACCATGGTTGCTCAGTCACAAGTGGGTTTTATTGACTTCATTGTGGAGCCCACCTTCACTGTGCTCACGG
ACATGACCGAGAAGATTGTGAGTCCATTAATTGACGAAAGCTCCCAGACTGGTGGGACAGGGCAGAGGAG
ATCAAGTTTGAACAGCATCAACTCATCAGATGCAAAGCGATCCGGTGTCAAGAGTTCTGGGTCAGATGGA
AGTGCTCCCATCAACAATTCTGTCATTCCTGTTGACTATAAGAGTTTTAAAGCCACTTGGACTGAGGTGG
TGCAGATCAATCGGGAGCGGTGGCGAGCCAAGGTACCCAAAGAAGAAAAAGCCAAGAAGGAAGCTGAAGA
GAAGGCTCGCCTGGCTGCTGAGGAAAAGCAAAAGGAAATGGAAGCCAAAAGCCAAGCTGAACAAGGCACA
ACCAGCAAAGGCGAGAAAAAGACATCAGGAGAAGCCAAAAGTCAAGTCAATGGAACACGCAAGGGTGATA
ACCCTCGTGGGAAGAACTCCAAAGGAGAAAAGGCAGGCGAAAAGCAGCAGAACGGTGACTTGAAAGACGG
TAAAAATAAGGCAGACAAGAAGGATCACTCCAACACCGGAAATGAGTCAAAGAAAACAGATGATCCAGAG
GAG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG209609 representing BC032277
Red=Cloning site Green=Tags(s)

MESPTKEIEEFESNSLKHLQPEQIEKIWLRLRGLRKYKKTSQRLRSLVKQLERGEASVVDLKKNLEYAAT
VLESVYIDETRRLLDTEDELSDIQSDAVPSEVRDWLASTFTRQMGMMLRRSDEKPRFKSIVHAVQAGIFV
ERMYRRTSNMVGLSYPPAVIDALKDVDTWSFDVFSLNEASGDHALKFIFYELLTRYDLISRFKIPISALV
SFVEALEVGYSKHKNPYHNLMHAADVTQTVHYLLYKTGVANWLTELEIFAIIFSAAIHDYEHTGTTNNFH
IQTRSDPAILYNDRSVLENHHLSAAYRLLQEDEEMNILVNLSKDDWREFRTLVIEMVMATDMSCHFQQIK
AMKTALQQPEAIEKPKALSLMLHTADISHPAKAWDLHHRWTMSLLEEFFRQGDREAELGLPFSPLCDRKS
TMVAQSQVGFIDFIVEPTFTVLTDMTEKIVSPLIDESSQTGGTGQRRSSLNSINSSDAKRSGVKSSGSDG
SAPINNSVIPVDYKSFKATWTEVVQINRERWRAKVPKEEKAKKEAEEKARLAAEEKQKEMEAKSQAEQGT
TSKGEKKTSGEAKSQVNGTRKGDNPRGKNSKGEKAGEKQQNGDLKDGKNKADKKDHSNTGNESKKTDDPE
E

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC032277
ORF Size 1895 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC032277, AAH32277
RefSeq Size 6154 bp
RefSeq ORF 1895 bp
Locus ID 18575
Gene Summary Calmodulin-dependent cyclic nucleotide phosphodiesterase with a dual-specificity for cAMP and cGMP, which are key regulators of many important physiological processes (PubMed:8810348). Exhibits high affinity for both cAMP and cGMP (By similarity). Modulates the amplitude and duration of the cAMP signal in sensory cilia in response to odorant stimulation, hence contributing to the generation of action potentials. Regulates smooth muscle cell proliferation. Regulates the stability of growth factor receptors, including PDGFRB (Probable).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
Customer Reviews 
Loading...