Gga1 (NM_145929) Mouse Tagged ORF Clone
CAT#: MG209645
- TrueORF®
Gga1 (tGFP-tagged) - Mouse golgi associated, gamma adaptin ear containing, ARF binding protein 1 (Gga1)
ORF Plasmid: DDK
"NM_145929" in other vectors (4)
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CNY 5,040.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | 4930406E12Rik; AU016030; AW209092 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG209645 representing NM_145929
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCCCGCGATGGAGCCGGAGACTCTGGAGGCACGAATCAACAGAGCCACAAATCCCCTGAACAAGG AGCTGAACTGGGCCAGCATCAACAGTTTCTGCGAGCAGCTCAACGAAGACTTTGAGGGGCCTCCACTTGC CACTCGCTTGCTGGCCCACAAGATCCAGTCCCCACAGGAATGGGAAGCCATCCAGGCCTTGACGGTTCTG GAGACGTGCATGAAGAGCTGCGGCAAGAGGTTCCATGATGAGGTGGGCAAGTTCCGCTTCCTCAACGAGC TCATCAAGGTTGTGTCTCCCAAGTACTTGGGCTCCCGGACATCTGAGAAGGTGAAGAGTAAGATCTTGGA GCTGCTGTACAGCTGGACGGTTTGCCTGCCTGAGGAGGTGAAGATTGCAGAAGCCTACCAGATGCTGAAG AAGCAGGGGATTGTGAAGTCGGACCCCAAGCTTCCAGAGGATGCCATCTTTCCCCTCCCCCCTCCCCGGC CCAAGAATGTGATCTTTGAAGATGAGGAGAAGTCCAAGATGCTGGCCCGCCTGCTGAAGAGCTCACACCC TGAGGACCTCCGGGCTGCCAATAAGCTCATCAAAGAGATGGTGCAGGAGGACCAGAAGCGGATGGAAAAG ATCTCCAAGCGGGTGAATGCCATCGAGGAGGTCAACAACAATGTGAAGTTGCTGACAGAGATGGTGATGA GCCACAGCCAGGGTGCTGCATCCAGCAGCAGTGAGGACCTCATGAAGGAACTGTACCAGCGCTGTGAGCG CATGCGACCCACACTCTTCCGACTGGCCAGTGACACGGAAGACAATGATGAGGCCTTAGCTGAGATCCTG CAGGCTAATGACAATCTCACCCAGGTGATCAACCTGTACAAGCAGCTGGTCCGGGGCGAGGAGGTCAACG GTGATGCCACAGCCAGCTCCATTCCTGGAAGCACGTCAGCCCTGCTGGACCTCTCAGGCCTGGACCTCCC TCCCCCGGGCACCACCCAGCCAGCCACGCCCACCCGCCCTGGCAACCAGAGCAGTCCTGAGCAGCTCAGT GCCTCGGTGTCCCTGCTTGATGACGAGCTCATGTCTCTGGGCCTAAGTGACCCGACACCACCTTCAGGCA CCAGCTCAGATAGTGTGGGGTGGGACAACTTCCAGTCATCAGATGGCACTGAATCCTCAGTCCCTCCTCC AGCCCAGGCCCCCAGCATGGACTGCCGACCCCCAGCCCAGGCCCCTCCACCAACGAGCAGCGGCCTGGAC GACCTGGACCTCTTGGGGAAAACCCTTATGCAGCAGGCTCTGCCTCCGGAAGCCCAGCAAGTGCGGTGGG AGAAGCAGCAGCCAGCCCCCCGGCTCACCCTCCGTGACCTGCAGAGTAAGAGCAGCTCGCCCAGCCCAGG AGCCGCCAGCCTCCTCCACACCACGTCCCCAGAGCCCCCTGGGCCTCCACCTCAGGCCACACCCACTGAG TTCTCCCTAACCAGCATCACTGTGCCCCTGGAGTCTATCAAACCCAGCAGCATCTTGCCAGTGACCGTTT ATGACCAGCATGGCTTCCGTGTCCTCTTCCATTTTGCTCGGGACCCACTGCCAGGGCGCTCCGATGTGCT GGTGGTGGTGGTCTCTATGCTGAGCACGGCGCCCCAGCCCATCCGGAACATCGTTTTCCAGTCAGCCGTC CCCAAGGTCATGAAGGTGAGGCTGCAGCCACCTTCCGGCACAGAGCTGCCAGCGTTCAACCCCATCGTCC ACCCCTCAGCCATCACCCAGGTCCTGCTCCTTGCTAACCCCCAGAAGGAGAAGGTTCGCCTCCGCTACAA GCTCATCTTCACTATGGGCGACCAGACCTACAATGAGATGGGAGATGTGGATCAGTTCCCCCCACCAGAG ACCTGGGGGAGCCTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG209645 representing NM_145929
Red=Cloning site Green=Tags(s) MEPAMEPETLEARINRATNPLNKELNWASINSFCEQLNEDFEGPPLATRLLAHKIQSPQEWEAIQALTVL ETCMKSCGKRFHDEVGKFRFLNELIKVVSPKYLGSRTSEKVKSKILELLYSWTVCLPEEVKIAEAYQMLK KQGIVKSDPKLPEDAIFPLPPPRPKNVIFEDEEKSKMLARLLKSSHPEDLRAANKLIKEMVQEDQKRMEK ISKRVNAIEEVNNNVKLLTEMVMSHSQGAASSSSEDLMKELYQRCERMRPTLFRLASDTEDNDEALAEIL QANDNLTQVINLYKQLVRGEEVNGDATASSIPGSTSALLDLSGLDLPPPGTTQPATPTRPGNQSSPEQLS ASVSLLDDELMSLGLSDPTPPSGTSSDSVGWDNFQSSDGTESSVPPPAQAPSMDCRPPAQAPPPTSSGLD DLDLLGKTLMQQALPPEAQQVRWEKQQPAPRLTLRDLQSKSSSPSPGAASLLHTTSPEPPGPPPQATPTE FSLTSITVPLESIKPSSILPVTVYDQHGFRVLFHFARDPLPGRSDVLVVVVSMLSTAPQPIRNIVFQSAV PKVMKVRLQPPSGTELPAFNPIVHPSAITQVLLLANPQKEKVRLRYKLIFTMGDQTYNEMGDVDQFPPPE TWGSL TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_145929 |
ORF Size | 1905 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_145929.2, NP_666041.1 |
RefSeq Size | 3034 bp |
RefSeq ORF | 1908 bp |
Locus ID | 106039 |
UniProt ID | Q8R0H9 |
Gene Summary | Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes (PubMed:25405894). Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (DXXLL) motif. Mediates export of the GPCR receptor ADRA2B to the cell surface (By similarity). Required for targeting PKD1:PKD2 complex from the trans-Golgi network to the cilium membrane (PubMed:25405894). Regulates retrograde transport of proteins such as phosphorylated form of BACE1 from endosomes to the trans-Golgi network (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC219936 | Gga1 (untagged) - Mouse golgi associated, gamma adaptin ear containing, ARF binding protein 1 (Gga1), (10ug) |
CNY 8,080.00 |
|
MR209645 | Gga1 (Myc-DDK-tagged) - Mouse golgi associated, gamma adaptin ear containing, ARF binding protein 1 (Gga1) |
CNY 4,728.00 |
|
MR209645L3 | Lenti ORF clone of Gga1 (Myc-DDK-tagged) - Mouse golgi associated, gamma adaptin ear containing, ARF binding protein 1 (Gga1) |
CNY 6,460.00 |
|
MR209645L4 | Lenti ORF clone of Gga1 (mGFP-tagged) - Mouse golgi associated, gamma adaptin ear containing, ARF binding protein 1 (Gga1) |
CNY 6,460.00 |