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Abr (BC059064) Mouse Tagged ORF Clone
CAT＃: MG209696
- TrueORF®
Abr (tGFP-tagged) - Mouse active BCR-related gene (cDNA clone MGC:69899 IMAGE:6825281)
ORF Plasmid: DDK
"BC059064" in other vectors (3)
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CNY 3,990.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG209696 representing BC059064
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAATCCTGCTCATCATTCGCTTCTGCTGTAACTGCACCTATGCTCTGCTGTACAAGCCAATTGACC GAGTCACCCGGAGCACCCTGGTCCTTCACGATCTGTTGAAGCACACACCCGTGGACCACCCGGACTACCC GCTGCTTCAGGATGCTCTCCGCATCTCCCAGAATTTCCTGTCTAGCATTAACGAGGACATTGATCCTCGC CGGACTGCAGTCACAACCCCCAAGGGAGAGACTCGGCAGCTGGTGAAGGACGGCTTCCTGGTGGAAATGT CTGAGAGCTCCCGGAAGCTGCGGCATGTCTTTCTCTTCACAGATGTTCTGCTGTGTGCCAAGCTAAAGAA GACCTCAGCAGGAAAGCATCAGCAGTATGACTGTAAGTGGTACATCCCTCTGGCTGACTTAGTGTTCCCA TCCCCTGAGGAGTCAGAGGCCAGTCCCCAGGTGCACCCCTTCCCAGACCACGAGCTCGAGGACATGAAAA CGAAGATCTCTGCCCTCAAGAGTGAGATCCAGAAGGAGAAAGCCAACAAAGGACAGAGCCGAGCCATTGA GCGCCTCAAAAAGAAGATGTTTGAGAATGAGTTCTTACTGCTGCTCAATTCCCCAACGATCCCTTTCCGG ATACACAATCGGAATGGAAAGAGTTACCTGTTTCTTCTGTCTTCTGACTATGAGAGGTCGGAGTGGAGAG AAGCGATTCAGAAGCTACAGAAGAAGGATCTCCAGGCCTTTGTCCTGAGCTCTGTGGAGCTCCAGGTGCT CACGGGATCCTGTTTCAAGCTTAGGACTGTGCACAACATTCCTGTCACCAGCAATAAAGATGATGATGAG TCTCCAGGACTGTACGGCTTCCTTCATGTCATCGTCCACTCTGCCAAGGGCTTTAAACAGTCAGCCAATC TTTACTGTACTCTGGAGGTGGATTCCTTTGGCTATTTTGTCAGCAAAGCCAAGACCAGGGTGTTTCGGGA CACGACAGAGCCCAAGTGGGATGAGGAGTTTGAGATTGAGCTGGAGGGTTCACAGTCCCTGAGGATCCTG TGTTATGAGAAGTGCTACGACAAGACCAAGGTCAACAAAGACAACAATGAGATTGTGGACAAGATCATGG GCAAGGGGCAGATCCAGCTGGATCCACAGACAGTAGAAAGCAAGAACTGGCATACGGACGTGATTGAAAT GAATGGGATCAAAGTGGAATTCTCCATGAAATTTACCAGCCGTGACATGAGCCTGAAGAGGACCCCATCC AAAAAGCAGACAGGCGTCTTTGGAGTGAAGATCAGCGTGGTGACCAAGCGGGAGCGCTCCAAGGTGCCCT ACATCGTCCGGCAATGCATAGAAGAGGTGGAGAAGAGGGGCATTGAGGAGGTTGGCATCTACAGGATATC AGGGGTGGCCACGGACATCCAGGCCCTGAAGGCTGTCTTTGATGCCAATAACAAGGACATCCTACTGATG CTGAGCGACATGGACATCAACGCCATCGCTGGGACCCTCAAGCTATACTTTCGGGAGCTGCCTGAGCCCC TCCTTACAGATAGACTTTATCCAGCCTTCATGGAGGGCATTGCCCTGTCAGACCCTGCTGCCAAGGAGAA CTGCATGATGCACCTACTCCGCTCCCTGCCTGACCCCAACCTCATCACCTTCCTTTTCCTGCTGGAACAC TTGAAAAGGGTTGCTGAGAAGGAGCCCATCAACAAGATGTCACTTCACAACCTTGCCACGGTGTTTGGCC CCACGTTACTGAGACCATCAGAAGTGGAGAGCAAAGCACATCTCACATCAGCTGCAGACATCTGGTCCCA TGATGTCATGGCCCAGGTCCAGGTCCTCCTCTACTACCTGCAGCATCCCCCCATTTCCTTCGCAGAACTG AAGCGGAACACACTGTACTTCTCCACAGACGTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG209696 representing BC059064
Red=Cloning site Green=Tags(s) MEILLIIRFCCNCTYALLYKPIDRVTRSTLVLHDLLKHTPVDHPDYPLLQDALRISQNFLSSINEDIDPR RTAVTTPKGETRQLVKDGFLVEMSESSRKLRHVFLFTDVLLCAKLKKTSAGKHQQYDCKWYIPLADLVFP SPEESEASPQVHPFPDHELEDMKTKISALKSEIQKEKANKGQSRAIERLKKKMFENEFLLLLNSPTIPFR IHNRNGKSYLFLLSSDYERSEWREAIQKLQKKDLQAFVLSSVELQVLTGSCFKLRTVHNIPVTSNKDDDE SPGLYGFLHVIVHSAKGFKQSANLYCTLEVDSFGYFVSKAKTRVFRDTTEPKWDEEFEIELEGSQSLRIL CYEKCYDKTKVNKDNNEIVDKIMGKGQIQLDPQTVESKNWHTDVIEMNGIKVEFSMKFTSRDMSLKRTPS KKQTGVFGVKISVVTKRERSKVPYIVRQCIEEVEKRGIEEVGIYRISGVATDIQALKAVFDANNKDILLM LSDMDINAIAGTLKLYFRELPEPLLTDRLYPAFMEGIALSDPAAKENCMMHLLRSLPDPNLITFLFLLEH LKRVAEKEPINKMSLHNLATVFGPTLLRPSEVESKAHLTSAADIWSHDVMAQVQVLLYYLQHPPISFAEL KRNTLYFSTDV TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC059064 |
| ORF Size | 1923 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC059064, AAH59064 |
| RefSeq Size | 4455 bp |
| RefSeq ORF | 1925 bp |
| Locus ID | 109934 |
| Gene Summary | Protein with a unique structure having two opposing regulatory activities toward small GTP-binding proteins. The C-terminus is a GTPase-activating protein domain which stimulates GTP hydrolysis by RAC1, RAC2 and CDC42. Accelerates the intrinsic rate of GTP hydrolysis of RAC1 or CDC42, leading to down-regulation of the active GTP-bound form. The central Dbl homology (DH) domain functions as guanine nucleotide exchange factor (GEF) that modulates the GTPases CDC42, RHOA and RAC1. Promotes the conversion of CDC42, RHOA and RAC1 from the GDP-bound to the GTP-bound form (By similarity). Functions as an important negative regulator of neuronal RAC1 activity (PubMed:20962234). Regulates macrophage functions such as CSF-1 directed motility and phagocytosis through the modulation of RAC1 activity (PubMed:17116687).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR209696 | Abr (Myc-DDK-tagged) - Mouse active BCR-related gene (cDNA clone MGC:69899 IMAGE:6825281) |
CNY 4,776.00 |
|
| MR209696L3 | Lenti ORF clone of Abr (Myc-DDK-tagged) - Mouse active BCR-related gene (cDNA clone MGC:69899 IMAGE:6825281) |
CNY 5,510.00 |
|
| MR209696L4 | Lenti ORF clone of Abr (mGFP-tagged) - Mouse active BCR-related gene (cDNA clone MGC:69899 IMAGE:6825281) |
CNY 5,510.00 |
