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Arhgap24 (BC023344) Mouse Tagged ORF Clone
CAT＃: MG209791
- TrueORF®
Arhgap24 (tGFP-tagged) - Mouse Rho GTPase activating protein 24 (cDNA clone MGC:32510 IMAGE:5064885)
ORF Plasmid: DDK
"BC023344" in other vectors (3)
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CNY 7,220.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | MGC38749 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG209791 representing BC023344
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACAGCCAATCATGAGAGCTATCTCCTCATGGCGAGCACCCAGAATGACATGGAAGACTGGGTGAAGT CCATCCGCCGAGTCATATGGGGACCTTTTGGAGGAGGTATTTTTGGACAGAAACTGGAAGACACAGTCCG ATATGAAAAGAGGTACGGGAACCGGCTGGCACCCATGCTGGTGGAGCAGTGTGTGGACTTCATCCGACAG CGGGGGCTGAAGGAAGAAGGCCTCTTCCGGCTGCCGGGCCAGGCGAACCTGGTCAAGGAGCTCCAGGATG CGTTCGACTGTGGAGAGAAGCCCTCCTTCGACAGCAACACCGACGTCCACACTGTGGCATCCCTGCTGAA ATTGTATCTCCGAGAACTTCCAGAGCCCGTCGTTCCTTATGCAAAGTATGAAGACTTTTTGTCCTGCGCC ACGCTGCTCAGTAAGGAGGAAGAAGCAGGTGTTAAGGAGCTAATGAAACAAGTGAAGAGTTTGCCTGTGG TTAACTACAACCTTCTGAAGTACATTTGCAGGTTCCTGGATGAAGTGCAGTCCTATTCAGGAGTTAACAA GATGAGCGCACAGAACTTGGCAACTGTCTTCGGTCCTAACATCCTGCGCCCAAAGGTGGAAGATCCCTTG ACCATCATGGAGGGCACTGTGGTGGTCCAGCAGTTAATGTCAGTGATGATTAGTAAGCATGATCGACTCT TCCCCAAAGACACAGAACCACAAAGCAAGCCCCAAGACGGCCCGAACAGCAATAATAACGATGGTCATAA GAAAGCCACCATGGGTCAGTTGCAGAACAAAGAAAACAATAATACCAAGGAGAGCCCCGTCAGAAGGTGC TCTTGGGACAAGCCTGAATCCCCCCAGAGAAGCAGCGTGGACAATGGATCCCCCACAGCCCTATCAGGCA GCAAAACCAACAGCCCAAGGAACAGTATCCACAAGCTGGATATTTCCAGGAGCCCCCCTCTCATGGTCAA AAAGAACCCAGCCTTCAACAAGGGCAGTGGGATTGTCACCAACGGGTCATTCAGCAGCAGCAATGCAGAA GGTGTCGAGAAGCCTCAAACTACTCCTAATGGGAGTTTACAAGCCAGAAGGACCTCTTCACTGAAGAGTT CTGGTACCAAAATGGGCACTCACAGTGTACAGAATGGCACTGTCCGCATGGGTATTCTGAACACTGACAC ACTAGGGAATTCCTTGAACGGCCGGAGCATGAGTTGGCTGCCCAATGGCTATGTGACCCTGAGAGATAAC AAACAGAAGGAGCCAGCAGGAGAGTCAGGCCAGCACAACAGACTCTCCACCTACGACAATGTCCACCAGC AGTTCTCCTCGATGAGCCTCGACGACAAGCACAGCGTGGACAGTGCCACGTGGTCCACCTCCTCCTGCGA AATCTCCCTCCCTGAGAACTCCAATTCCTGCCGCTCCTCTACCACCACGTGCCCAGAGCAAGACTTCTAC GTTGGGAACTTTGAGGACCCTGTTTTAGACGGCCCACCTCAGGATGATCTTTCCCATCCCGGGGACTATG AGAACAAAAGTGACCGCAGGAGCGTAGGGGGCCGAAGCAGCCGCGCCACGAGCAGCAGCGATAACAGCGA GACATTCGTGGGCAACACCAGCAGCAACCACAGTGCACTTCACAGTTTAGTTTCCAGCCTGAAGCAGGAG ATGACCAAACAGAAGATCGAGTATGAGTCCAGGATAAAAAGCTTAGAACAGCGGAATTTGACTTTGGAAA CGGAAATGCTGAGCCTCCATGACGAACTGGATCAAGAGAGGAAAAAGTTCACCATGATAGAAATCAAGAT GAGAAACGCTGAGCGCGCAAAGGAGGATGCGGAGAAAAGGAACGACATGCTCCAGAAGGAGATGGAGCAG TTCTTCTCCACATTTGGAGACCTGACAGTGGAACCCAGGAGAAGCGAGAGAGGAAACACAATCTGGATCC AG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG209791 representing BC023344
Red=Cloning site Green=Tags(s) MTANHESYLLMASTQNDMEDWVKSIRRVIWGPFGGGIFGQKLEDTVRYEKRYGNRLAPMLVEQCVDFIRQ RGLKEEGLFRLPGQANLVKELQDAFDCGEKPSFDSNTDVHTVASLLKLYLRELPEPVVPYAKYEDFLSCA TLLSKEEEAGVKELMKQVKSLPVVNYNLLKYICRFLDEVQSYSGVNKMSAQNLATVFGPNILRPKVEDPL TIMEGTVVVQQLMSVMISKHDRLFPKDTEPQSKPQDGPNSNNNDGHKKATMGQLQNKENNNTKESPVRRC SWDKPESPQRSSVDNGSPTALSGSKTNSPRNSIHKLDISRSPPLMVKKNPAFNKGSGIVTNGSFSSSNAE GVEKPQTTPNGSLQARRTSSLKSSGTKMGTHSVQNGTVRMGILNTDTLGNSLNGRSMSWLPNGYVTLRDN KQKEPAGESGQHNRLSTYDNVHQQFSSMSLDDKHSVDSATWSTSSCEISLPENSNSCRSSTTTCPEQDFY VGNFEDPVLDGPPQDDLSHPGDYENKSDRRSVGGRSSRATSSSDNSETFVGNTSSNHSALHSLVSSLKQE MTKQKIEYESRIKSLEQRNLTLETEMLSLHDELDQERKKFTMIEIKMRNAERAKEDAEKRNDMLQKEMEQ FFSTFGDLTVEPRRSERGNTIWIQ TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC023344 |
| ORF Size | 1962 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC023344, AAH23344 |
| RefSeq Size | 2732 bp |
| RefSeq ORF | 1964 bp |
| Locus ID | 231532 |
| Gene Summary | Rho GTPase-activating protein involved in cell polarity, cell morphology and cytoskeletal organization. Acts as a GTPase activator for the Rac-type GTPase by converting it to an inactive GDP-bound state. Controls actin remodeling by inactivating Rac downstream of Rho leading to suppress leading edge protrusion and promotes cell retraction to achieve cellular polarity. Able to suppress RAC1 and CDC42 activity in vitro. Overexpression induces cell rounding with partial or complete disruption of actin stress fibers and formation of membrane ruffles, lamellipodia, and filopodia. Isoform 2 is a vascular cell-specific GAP involved in modulation of angiogenesis (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR209791 | Arhgap24 (Myc-DDK-tagged) - Mouse Rho GTPase activating protein 24 (cDNA clone MGC:32510 IMAGE:5064885) |
CNY 4,872.00 |
|
| MR209791L3 | Lenti ORF clone of Arhgap24 (Myc-DDK-tagged) - Mouse Rho GTPase activating protein 24 (cDNA clone MGC:32510 IMAGE:5064885) |
CNY 8,460.00 |
|
| MR209791L4 | Lenti ORF clone of Arhgap24 (mGFP-tagged) - Mouse Rho GTPase activating protein 24 (cDNA clone MGC:32510 IMAGE:5064885) |
CNY 8,460.00 |
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