Flrt2 (BC096471) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG209836 representing BC096471 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCCTACAGACTACAAAGTGGCCCGGCCGTGGGGCTTTCATCCTCAAATTTTGGCTCATCATTTCCC TGGGACTCTACTTACAAGTGTCAAAACTCCTGGCCTGCCCTAGTGTATGCCGCTGTGACAGGAACTTTGT CTACTGTAACGAGAGAAGCTTGACCTCAGTGCCTCTTGGGATCCCGGAGGGCGTAACCGTACTCTACCTC CACAACAACCAAATTAATAATGCTGGCTTTCCTGCAGAGCTGCACAATGTCCAGTCAGTGCACACTGTCT ACCTTTATGGCAACCAGTTGGATGAGTTCCCCATGAACCTTCCCAAGAATGTCAGAGTTCTCCATCTGCA GGAAAACAACATTCAGACCATCTCGCGGGCTGCTCTCGCTCAGCTCCTCAAGCTGGAAGAACTCCACCTG GACGACAACTCCATATCTACAGTGGGAGTAGAAGACGGAGCGTTCCGGGAGGCGATTAGCCTCAAACTGT TGTTTTTATCGAAGAATCACCTGAGTAGCGTGCCTGTTGGGCTTCCTGTAGACTTGCAAGAGCTGAGAGT GGATGAAAACCGAATTGCCGTCATATCAGACATGGCCTTTCAGAACCTCACAAGCTTGGAGCGCCTGATC GTGGATGGGAATCTTCTGACCAACAAGGGCATTGCTGAGGGTACCTTCAGTCATCTCACCAAGCTCAAGG AATTTTCTATAGTCCGGAACTCGCTCTCCCACCCACCTCCTGATCTCCCAGGTACGCATCTGATCAGGCT CTACTTGCAGGATAACCAGATAAACCATATCCCATTGACAGCCTTCGCAAACCTCCGTAAGCTGGAAAGG CTAGATATATCCAACAACCAGCTACGAATGTTAACTCAAGGAGTCTTCGATCATCTCTCCAACCTGAAAC AACTCACTGCGCGGAATAACCCTTGGTTTTGTGACTGCAGCATTAAATGGGTCACAGAATGGCTCAAGTA TATCCCTTCTTCTCTCAACGTGCGTGGTTTCATGTGCCAAGGTCCTGAGCAAGTCCGCGGGATGGCTGTC AGGGAGTTGAATATGAATCTTTTGTCTTGTCCCACCACGACTCCTGGCCTACCTGTCTTTACCCCAGCTC CAAGTACCGTTTCTCCAACAACTCAGTCTCCTACTCTCTCTGTTCCAAGCCCCAGCAGAGGCTCTGTGCC TCCAGCTCCTACCCCATCGAAACTTCCCACCATCCCTGATTGGGATGGCAGAGAAAGAGTGACCCCACCT ATTTCTGAAAGGATCCAACTTTCCATCCACTTTGTTAATGATACTTCGATTCAAGTCAGCTGGCTGTCTC TCTTTACTGTGATGGCCTACAAACTGACATGGGTGAAAATGGGCCACAGTCTCGTAGGGGGCATCGTTCA GGAACGAATTGTCAGTGGTGAGAAGCAACACCTGAGCTTGGTTAATTTAGAGCCCAGATCCACGTATAGG ATTTGTTTAGTGCCGCTGGATGCGTTCAACTACCGCACTGTGGAAGATACCATCTGTTCGGAGGCTACCA CCCATGCCTCTTATTTGAACAACGGCAGCAACACTGCTTCTAGCCATGAGCAGACGACTTCCCACAGTAT GGGCTCCCCTTTTCTGCTCGCAGGCTTGATTGGGGGCGCAGTGATTTTTGTGCTCGTTGTCTTGCTCAGC GTCTTTTGCTGGCACATGCACAAAAAGGGACGCTACACCTCTCAGAAGTGGAAATACAACCGGGGCCGAC GGAAAGACGACTATTGTGAAGCGGGTACCAAAAAAGACAACTCCATCTTGGAGATGACAGAAACAAGTTT TCAGATTGTCTCCTTAAATAACGATCAGCTCCTTAAAGGAGATTTCAGACTGCAGCCCATTTATACCCCA AATGGGGGCATTAATTACACAGACTGCCACATCCCCAACAACATGAGATACTGCAACAGCAGTGTGCCAG ATCTAGAGCATTGCCATACG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG209836 representing BC096471 Red=Cloning site Green=Tags(s) MGLQTTKWPGRGAFILKFWLIISLGLYLQVSKLLACPSVCRCDRNFVYCNERSLTSVPLGIPEGVTVLYL HNNQINNAGFPAELHNVQSVHTVYLYGNQLDEFPMNLPKNVRVLHLQENNIQTISRAALAQLLKLEELHL DDNSISTVGVEDGAFREAISLKLLFLSKNHLSSVPVGLPVDLQELRVDENRIAVISDMAFQNLTSLERLI VDGNLLTNKGIAEGTFSHLTKLKEFSIVRNSLSHPPPDLPGTHLIRLYLQDNQINHIPLTAFANLRKLER LDISNNQLRMLTQGVFDHLSNLKQLTARNNPWFCDCSIKWVTEWLKYIPSSLNVRGFMCQGPEQVRGMAV RELNMNLLSCPTTTPGLPVFTPAPSTVSPTTQSPTLSVPSPSRGSVPPAPTPSKLPTIPDWDGRERVTPP ISERIQLSIHFVNDTSIQVSWLSLFTVMAYKLTWVKMGHSLVGGIVQERIVSGEKQHLSLVNLEPRSTYR ICLVPLDAFNYRTVEDTICSEATTHASYLNNGSNTASSHEQTTSHSMGSPFLLAGLIGGAVIFVLVVLLS VFCWHMHKKGRYTSQKWKYNRGRRKDDYCEAGTKKDNSILEMTETSFQIVSLNNDQLLKGDFRLQPIYTP NGGINYTDCHIPNNMRYCNSSVPDLEHCHT TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC096471
ORF Size	1980 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC096471, AAH96471
RefSeq Size	2968 bp
RefSeq ORF	1982 bp
Locus ID	399558
Gene Summary	Functions in cell-cell adhesion, cell migration and axon guidance. Mediates cell-cell adhesion via its interactions with ADGRL3 and probably also other latrophilins that are expressed at the surface of adjacent cells (PubMed:21350012, PubMed:25728924 PubMed:25374360). May play a role in the migration of cortical neurons during brain development via its interaction with UNC5D (PubMed:21673655). Mediates axon growth cone collapse and plays a repulsive role in neuron guidance via its interaction with UNC5D, and possibly also other UNC-5 family members (PubMed:21673655, PubMed:25728924). Plays a role in fibroblast growth factor-mediated signaling cascades (PubMed:16872596). Required for normal organization of the cardiac basement membrane during embryogenesis, and for normal embryonic epicardium and heart morphogenesis (PubMed:21350012).[UniProtKB/Swiss-Prot Function]

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