Bfsp1 (BC057580) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	CP95
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG209865 representing BC057580 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTACCGCCGCAGCTACGTCTTCCAGGCCCGCCAGGAGCGCTACGAGCGCGCCCAGCCCGCGGGCCCCG CCGCCCAGCCCGGGGGAACCGCGCCCGGCCTGGCAGCGCTGCAGGCGCTGGGCGAGCGCGTGGCAGTCCA GGTCCAGCGGGCCCGCGCGCTCCAGCAGCGCCACGCCGGCCTGCGGCGGCAGCTCGACGCCTTCCAGCGC CTGGGCGAGCAGCCCGGGCCCGAGGACGCGCTCGCCCGCCACGTCGAGGCCAATCTGCAGCGCGCCCGGG ACCTGACAGCCGAGCACGCGCGGCTCGAGAGGCAGGAGGCCGAGGCGCAGCGCGCGCTCGACGAGTTCCG CAGCAAATACGAAAATGAGTGTGAATGTCAGTTGGTGCTAAAGGAAATGCTGGAACGGCTTAACAAGGAA GCCGATGAAGCCTTATTGCGTAACCTGCACCTTCAGTTGGAGGCACAGTTTCTGCAGGCAGATATCAGTG TGGCCAAGGACAGGTACAAGAAGAATCTTCTGGAGATTCAGACCTACATCACCGTCCTGCAGCAGATCGT ACAGACTGCTCCTCAAGTGTCCCTGGTTACTGGGATGAGGGAGGAGAAGCTGTTCACAGAACGGGAAGTG GCTGCCCTGCAGAACCAGCTGGAGGAGGGCCGAGAGGCAGTCACCCACCTGCAGGCACAGAAAGCTGAGC TGCAGGCCCAGACCACGGCCCTGGAACAAGCTATTAAGCATGCTCATGAGTGTTACGATGAGGAGCTACA GCTTTACAACGAGCAGATCGAAAACCTTCGGAAGGAGATCGAGGAGGCGGAGCGGTCTCTGGAGAGGTCG TCCTATGACTGCAGGCAGCTAGCCGTGGCCCAGCAGACGCTGAGGAATGAGCTAGACCGTTATCATCGGA TCATTGAGATTGAAGGCAGCAGGCTGAGCTCTGTTTTCATTGAGACGCCCATCTCTCTGATCACCCCGAG CCATGGGGCCCCTCTCAGCCTTGGATCCAGTGTGAAAGATCTTGCCAGGGCCGTGCAGGATATTACAGCA GCTAAACCGAGACAGAAGGCCCTCCCCAAGAGCCTTCCAAAAAGAAAGGAGATTATAGCTCAAGATAAGG TGGAGGAAACTCTGGAAGATGCACCATTAAAACCTCCGCAGGAGCCAAAGGCACTGCAGGTGGAACGGAA AGCAGAAGGTGGCTCTCAGCCCGGAGCAGGGGGAGGACATGGAGTAAGTCCCACACAAGAAGGGGGTCCT GAGGATGTGCCAGACGGGGGCCAGATAAGCAAAGCCTTTGGGAAACTGTGCAAAGTAGTGAAAGAGAGAG TGAGCGGCCACAAAGAGCCCGAGCCTGAGCCGCCCACGGACCTTTTCACCAAAGGACGGCATGTACTCGT CACAGGGGAGTCTAGTTTTGTTGACCCCGAGTTCTACTCCTCCTCCATCCCAGCTAGAGGTGGGGTGGTG ATTTCCATTGAGGAAGACTCTATGCATCATGACGGCCACGTAGAGCCCTCTCCTGGACAGCCCATGCCAC CTGTGGAGAATGGACAGGGCGTTCCCCAGGGCAGGGAAGGTGACCACTCAAACCATCAGCAGGGTACAGA CAAGAATGGGTTACGGGCCAAGGAACCAAAAGACCTGGAGGAAAAAGATGATGATGGCAAGAAGGAAGCT GAGGGGAGCAGGAGACCCTGTCCTGTGATCATACCTGGTCCTGATGAACCATCTACATCCCATTCACAGA CGTCTGGGTCCAATCAGGGTGGACCAGTGGGGCCTGCGAGTAAGAGCAGTAGCCTGCTGGCAAAGGGCCC TTCGAAGGCTCTGTCTATTAAGAAGGTGGAGGTGGTGGAATCCATTGAGAAGATTTCAACAGAGAGTATT CAGACATATGAAGAGACTTCCGTAATTGTGGAGACTCTGATTGGGAAGTCAAAGGGCAACAAGAAACTGG GAGAGAAGAGCTTGCCAGACACAAGGGCT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG209865 representing BC057580 Red=Cloning site Green=Tags(s) MYRRSYVFQARQERYERAQPAGPAAQPGGTAPGLAALQALGERVAVQVQRARALQQRHAGLRRQLDAFQR LGEQPGPEDALARHVEANLQRARDLTAEHARLERQEAEAQRALDEFRSKYENECECQLVLKEMLERLNKE ADEALLRNLHLQLEAQFLQADISVAKDRYKKNLLEIQTYITVLQQIVQTAPQVSLVTGMREEKLFTEREV AALQNQLEEGREAVTHLQAQKAELQAQTTALEQAIKHAHECYDEELQLYNEQIENLRKEIEEAERSLERS SYDCRQLAVAQQTLRNELDRYHRIIEIEGSRLSSVFIETPISLITPSHGAPLSLGSSVKDLARAVQDITA AKPRQKALPKSLPKRKEIIAQDKVEETLEDAPLKPPQEPKALQVERKAEGGSQPGAGGGHGVSPTQEGGP EDVPDGGQISKAFGKLCKVVKERVSGHKEPEPEPPTDLFTKGRHVLVTGESSFVDPEFYSSSIPARGGVV ISIEEDSMHHDGHVEPSPGQPMPPVENGQGVPQGREGDHSNHQQGTDKNGLRAKEPKDLEEKDDDGKKEA EGSRRPCPVIIPGPDEPSTSHSQTSGSNQGGPVGPASKSSSLLAKGPSKALSIKKVEVVESIEKISTESI QTYEETSVIVETLIGKSKGNKKLGEKSLPDTRA TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC057580
ORF Size	1991 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC057580, AAH57580
RefSeq Size	2414 bp
RefSeq ORF	1991 bp
Locus ID	12075
Gene Summary	Required for the correct formation of lens intermediate filaments as part of a complex composed of BFSP1, BFSP2 and CRYAA (By similarity). Involved in altering the calcium regulation of MIP water permeability (By similarity).[UniProtKB/Swiss-Prot Function]

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